AptaDB: Result

Interaction ID: 963

Aptamer sequence: GTGCGAAGCGCCTCCACTGTACATCCACTCCTTCTGCC

Target unique ID: Other-12

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_877	WKB-51	Other	P48 protein of Mycoplasma bovis (M. bovis)	20.81 nM	P48 protein dissolved in 100 μL of 0.05 carbonate-bicarbonate buffer	24417693

Aptamer Sequence:	GTGCGAAGCGCCTCCACTGTACATCCACTCCTTCTGCC
The optimal secondary structure in dot-bracket notation:	((((...))))...........................
The centroid secondary structure in dot-bracket notation:	......................................

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_877	Description	WKB-51
Aptamer chemistry	DNA	Length	38 nt
GC content	60.5%	Molecular weight	11,486.37 Da
Molarity of 1 μg/μl solution	87.06 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Target detection
Sequence	GTGCGAAGCGCCTCCACTGTACATCCACTCCTTCTGCC
Applications	Detection of antibodies to mycoplasma bovis in serum

Type	Detail	Type	Detail
Target ID	Other-12	Name	P48 protein of Mycoplasma bovis (M. bovis)

	Interaction ID	963
	Target type	Other
	Target unique ID	Other-12
	Activity	20.81 nM
	Binding Conditions/Buffer	P48 protein dissolved in 100 μL of 0.05 carbonate-bicarbonate buffer
	Assay	Dose-dependent i-ELAA to determine the dissociation constant (Kd) of aptamers
	PubMed ID	24417693

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_868	DNA	CCCAGAAACAGCACCCTTACATCGACTGGACCTTCCGCG	58.97%
Apta_358	DNA	GGGAATCGCCACCCGACGCAGGGTTTCCC	57.89%
Apta_602	DNA	GGGCGAGAACAGTGTCCCAGTCGCTAGTTTTCTCTGCCC	56.41%
Apta_360	DNA	GGGAACGCATACGCCGCAGTATTTCCC	55.26%
Apta_361	DNA	GGGAACGCCCGTATTGCTCGGTTCCC	55.26%
Apta_1084	DNA	TTCCCTCCTCTGTTGTTCCCCCCTCCTCTC	55.26%
Apta_221	DNA	GTCCTAGCAGACACTCGGCCATCACGCCCATTGGTTTGCA	55.00%
Apta_1215	DNA	GTCGAATCAGCACCTCTGCATAGGTTACGTTTATACTGCG	55.00%
Apta_1127	DNA	TCGGGCGAGTCGTCTGCGAGGCAACAAGCTCGTAATCCGCATCGTCCTCCC	54.90%
Apta_796	DNA	GTGCTGGATGTCACCTCCACTCACCACCTCTTCTCCGCTCCTCGTCATGACACATCCAG	54.24%
Apta_1126	DNA	TCGGGCGAGTCGTCTGCGGGCGGATAGACTACGACACCGCATCGTCCTCCC	52.94%
Apta_227	DNA	ACGCGCCTATGGTGCATCGCATGCCGACC	52.63%
Apta_359	DNA	GGGAACGCGTTATTGGGTAACTTCCC	52.63%
Apta_362	DNA	GGGAACGCACCGATCGCAGGTTTCCC	52.63%
Apta_56	DNA	GCAGCTAAGCAGGCGGCTCACAAAACCATTCGCATGCGGC	52.50%
Apta_165	DNA	TGGACGAACTGCCCTCAGCTACTTTCATGTTGCTGACGCA	52.50%
Apta_167	DNA	TGCTCCTTGGCCTTAGCCCTGGAACCATCAATCCTCTTCG	52.50%
Apta_387	DNA	CTTTAATGCCCTTCACTAGGTCTAAGCTACTACTTACCCG	52.50%
Apta_873	DNA	CCACCGAGCCTACCACATGTGACATCCCAGGACATAGCTG	52.50%
Apta_876	DNA	GTTGCGAAAGACAACGAATGCTTTGCCTGCCATAATTTGC	52.50%

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