AptaDB: Result

Interaction ID: 492

Aptamer sequence: CGGGGAGTTGGGAGGGGGGGCAAAGCCTGTGTATTTATCTTCTGT

Target unique ID: Q7AP54

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_487	C1	Protein	HBP2_LISMO	293 nM	20 mM Tris-AcOH (pH 8.0), 100 mM NaCl, 200 mM KCl, 5 mM MgCl2, 0.5% Triton X-100, and 5% DMSO	22954361

Aptamer Sequence:	CGGGGAGTTGGGAGGGGGGGCAAAGCCTGTGTATTTATCTTCTGT
The optimal secondary structure in dot-bracket notation:	..(((((.((((.....((((...)))).....)))).)))))..
The centroid secondary structure in dot-bracket notation:	..(((((.(((......((((...))))......))).)))))..

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_487	Description	C1
Aptamer chemistry	DNA	Length	45 nt
GC content	55.6%	Molecular weight	14,075.00 Da
Molarity of 1 μg/μl solution	71.05 μM	Number of G-quadruplexes	3
G-Score	62	Function	Aptasensor
Sequence	CGGGGAGTTGGGAGGGGGGGCAAAGCCTGTGTATTTATCTTCTGT
Applications	Peroxidase activity and strong binding affinity to hemin

Type	Detail	Type	Detail
Uniprot ID	Q7AP54	Protein name	Hemin/hemoglobin-binding protein 2 (Hn/Hb-binding protein 2) (P64) (Surface virulence-associated protein A)
Gene name(s)	hbp2 svpA lmo2185	Organism	Hemin/hemoglobin-binding protein 2 (Hn/Hb-binding protein 2) (P64) (Surface virulence-associated protein A)
Status	reviewed in uniprot database	Length	569
Mass	63,380	Activity regulation	Is overexpressed in mecA, clpC and clpP mutants
Involvement in disease	N/A	Catalytic activity	N/A
PDB ID(s)	4MYP;4NLA;
Function	Acts as an extracellular and cell wall-bound hemophore; scavenges host heme and hemoglobin from the environment and also serves as a cell wall receptor for both.

	Interaction ID	492
	Target type	Protein
	Target unique ID	Q7AP54
	Activity	293 nM
	Binding Conditions/Buffer	20 mM Tris-AcOH (pH 8.0), 100 mM NaCl, 200 mM KCl, 5 mM MgCl2, 0.5% Triton X-100, and 5% DMSO
	Assay	CD spectra of truncated aptamers (4 μM) in PB buffer without (black line) and with (red line) K+
	PubMed ID	22954361

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_754	DNA	GGGCGGGGAGTAGGGAGAGGGGTTTCCATCGGCGACAGAGGAGTTATGTGTGT	60.38%
Apta_335	DNA	ACCGGGCGTACACCGTCGCGGCACATGTCTGAATGCGTTTAGTCTCTGTG	58.00%
Apta_756	DNA	GGGGGACACGGAGGTGGTGGAAAGGCTAAGATTTGATGATGAGTAGTGTGGT	57.69%
Apta_609	DNA	GCTGCGAGGTGGGTGGGTGGGAGCAATTGGTCCTCGCTTAGCTTCTACGGTGGGCTATCT	56.67%
Apta_610	DNA	GCTGTGAGGTGGGTGGGTGGGAGCAATTGGTCCTCGCTTAGCTTCTACGGTGGGCTATCT	56.67%
Apta_611	DNA	GCTGCGAGGTGGGTGGGTGGGAGCAATTGGTCCTTGCTTAGCTTCTACGGTGGGCTATCT	56.67%
Apta_45	DNA	CGGGTGGGAGTTGTGGGGGGGGGTGGGAGGGTTCTTTGTTTGATCTTTCTCGCTGCCTGGCCCTAGAGTG	54.29%
Apta_783	DNA	AAGGGGTTGGGTGGGTTTATACAAATTAATTAATATTGTATGGTATATTT	54.00%
Apta_50	DNA	AGTGGCGAGGGGGGTAGGTAAAGTATCCTTAGTGTGTTG	53.33%
Apta_484	DNA	CAGGGAGGGACAGGGCGGGAATTTGTATGATGGTTCGTTTGCTCG	53.33%
Apta_565	DNA	GCGGGGTTGGGCGGGTGGGTTCGCTGGGCAGGGGGCGAGTG	53.33%
Apta_691	DNA	CGACGCGCGTTGGTGGTGGATGGTGTGTTACACGTGTTGT	53.33%
Apta_861	DNA	CAGGTTGGTCTGGTTGGGTTTGGCTCCTGTGTACGCAACCTG	53.33%
Apta_869	DNA	ACTAGGACGCTTGGGAGGGGGGGTGGGGTGTCCGGTCGCG	53.33%
Apta_870	DNA	CCGGGGAATCTAGGGGAGGGCGGGTGGGTCAATTGAGCCG	53.33%
Apta_958	DNA	CGCGTGAGCGGGGAGGCGATGCCCAGGCTAACTTGACTCA	53.33%
Apta_1014	DNA	TGGGGGTTGGGTGGATAGGCTGGCGTCGGGGCAGGTCAGT	53.33%
Apta_1185	DNA	CAGGGGGTTTTGGTGGGGGGGGCTGGGTTGTCTTGGGGGTGGGCT	53.33%
Apta_1186	DNA	AGGGGGTTTTGGTGGGGGGGGCTGGGTTGTCTTGGGGGTGGGCT	53.33%
Apta_1230	DNA	GGCGATAGGCAGTGTTGCGGGGTCGGAGAGCGAGGTAATAGCGTGTATGGGTGCTGTGTG	53.33%

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