AptaDB: Result

Interaction ID: 879

Aptamer sequence: CGCAGCAAGGAGATTCGAGGGGGAAGGTTTGTTATAGGGGTTAATGGA

Target unique ID: HB-8065

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_795	ZL-7c	Cell	HCC cell (HepG2/MDR)	86.26 nM	DPBS with glucose (4.5 mg/mL), MgCl2 (5 mM), BSA (1 mg/mL), and yeast tRNA (0.1 mg/mL)) at 37 °C in an orbital shaker for 50 min	34779207

Aptamer Sequence:	CGCAGCAAGGAGATTCGAGGGGGAAGGTTTGTTATAGGGGTTAATGGA
The optimal secondary structure in dot-bracket notation:	...((((((....(((......)))..))))))...............
The centroid secondary structure in dot-bracket notation:	...((((((....(((......)))..))))))...............

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_795	Description	ZL-7c
Aptamer chemistry	DNA	Length	48 nt
GC content	50.0%	Molecular weight	15,096.69 Da
Molarity of 1 μg/μl solution	66.24 μM	Number of G-quadruplexes	2
G-Score	18	Function	Targeted therapy
Sequence	CGCAGCAAGGAGATTCGAGGGGGAAGGTTTGTTATAGGGGTTAATGGA
Applications	Precise diagnosis and target theranostics of multidrug-resistant HCC

Type	Detail	Type	Detail
ATCC ID	HB-8065	Cell name	HCC cell (HepG2/MDR)
Category	Human cells	Organism	Homo sapiens, human
Product type	N/A	Cell type	N/A
Morphology	epithelial-like	Tissue	Liver
Disease	Carcinoma; Hepatocellular	Product format	Frozen
Antigen expression	N/A	Genes expressed	Alpha-fetoprotein (AFP, alpha fetoprotein); albumin; alpha2 macroglobulin (alpha-2-macroglobulin)
Cell description	Hep G2 [HEPG2] is a cell line exhibiting epithelial-like morphology that was isolated from a hepatocellular carcinoma of a 15-year-old, White, male youth with liver cancer. The cell line was deposited by the Wistar Institute and is a suitable transfection host. Expression markers include insulin; insulin-like growth factor II (IGF II).
Application(s)	3D cell culture; Cancer research; High-throughput screening; Toxicology

	Interaction ID	879
	Target type	Cell
	Target unique ID	HB-8065
	Activity	86.26 nM
	Binding Conditions/Buffer	DPBS with glucose (4.5 mg/mL), MgCl2 (5 mM), BSA (1 mg/mL), and yeast tRNA (0.1 mg/mL)) at 37 °C in an orbital shaker for 50 min
	Assay	Quantification analysis of apparent Kd of truncated ZL-7c on target HepG2/MDR cells
	PubMed ID	34779207

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_794	DNA	ATCCAGAGTGACGCAGCAAGGAGATTCGAGGGGGAAGGTTTGTTATAGGGGTTAATGGACACGGTGGCTTAGT	65.75%
Apta_488	DNA	CGACCAGGGTGGGAGGGGGACGGGCTGGCGGCGCGCGGTAATG	58.33%
Apta_691	DNA	CGACGCGCGTTGGTGGTGGATGGTGTGTTACACGTGTTGT	58.33%
Apta_287	DNA	CAGCACCGACCTTGTGCTTTGGGAGTGCTGGTCCAAGGGCGTTAATGGACA	56.86%
Apta_745	DNA	GGTTGCATGCCGTGGGGAGGGGGGTGGGTTTTATAGCGTACTCAG	56.25%
Apta_813	DNA	TAGAGATATGACAGCGGGGAAGGTTAAGAGGCGCTAGGAG	56.25%
Apta_1255	DNA	GGCGCATGATCATTCGGTGGGTAAGGTGGTGGTAACGTTG	56.25%
Apta_988	DNA	AAGGATGACTGACGACGATGGGAATGGAGAGGAAGGGGATATAGGTATGTTGGATGTAG	55.93%
Apta_756	DNA	GGGGGACACGGAGGTGGTGGAAAGGCTAAGATTTGATGATGAGTAGTGTGGT	55.77%
Apta_150	DNA	CCGTAGGTTCGGGGCGGAGTGGTCCGGAAGGTGGCGTGG	54.17%
Apta_341	DNA	TCGGGGTTTGGGGGTTCGGGGTCGGTTCGGTTTCTTCTAATGGA	54.17%
Apta_343	DNA	AACAGAGGGACAAACGGGGGAAGATTTGACGTCGACGAC	54.17%
Apta_960	DNA	CCCGAGGCACGAGGGGGAACGTATCGTCATGTGCCGGCCA	54.17%
Apta_962	DNA	CCACACCGAGATGTTGTGACGGTTCCGATTGGCAAACTGA	54.17%
Apta_1091	DNA	CCATGGTAGGTATTGCTTGGTAGGGATAGTGGGCTTGATG	54.17%
Apta_1092	DNA	CCCATGGTAGGTATTGCTTGGTAGGGATAGTGGGCTTGATG	54.17%
Apta_753	DNA	GGGCACAGACGGAAGATGAGAATTGTGGGGCTTAGTATAGTGAGGTGCGTGT	53.85%
Apta_1230	DNA	GGCGATAGGCAGTGTTGCGGGGTCGGAGAGCGAGGTAATAGCGTGTATGGGTGCTGTGTG	53.33%
Apta_140	DNA	GGCAGGAAGACAAACACGATGGGGGGGTATGATTTGATGTGGTTGTTGCATGATCGTGGTCTGTGGTGCTGT	52.78%
Apta_615	DNA	ACGAAGAAACAGGGCGCTTCGAACGAACATGGGCAGGGTGGGAAGTTTTAAAGTGGTAC	52.54%

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