Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 1033
Aptamer sequence: CACCTAATACGACTCACTATAGCGGATCCGAAGGGGGCGCGAGGTGTAAGGGTGTGGGGTGGTGGGTGGGCCTGGCTCGAACAAGCTTGC
Target unique ID: P05413
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_950 | N13 | Protein | FABPH_HUMAN | 0.0743 μM | 50 mM sodium phosphate buffer, pH 7.4, 50 mM NaCl, 5 mM KCl, 2.5 mM MgCl2 | 27545084 |
Structure information of aptamer
| Aptamer Sequence: | CACCTAATACGACTCACTATAGCGGATCCGAAGGGGGCGCGAGGTGTAAGGGTGTGGGGTGGTGGGTGGGCCTGGCTCGAACAAGCTTGC |
| The optimal secondary structure in dot-bracket notation: | (((((.((((....((((...(((..(((....))).)))..)))).....)))).)))))....((((((.((.......)).)))))) |
| The centroid secondary structure in dot-bracket notation: | (((((.((((....((((...(((..(((....))).)))..)))).....)))).)))))............................. |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_950 | Description | N13 |
| Aptamer chemistry | DNA | Length | 90 nt |
| GC content | 61.1% | Molecular weight | 28,092.91 Da |
| Molarity of 1 μg/μl solution | 35.60 μM | Number of G-quadruplexes | 3 |
| G-Score | 62 | Function | Targeted binding |
| Sequence | CACCTAATACGACTCACTATAGCGGATCCGAAGGGGGCGCGAGGTGTAAGGGTGTGGGGTGGTGGGTGGGCCTGGCTCGAACAAGCTTGC | ||
| Applications | Binding to target proteins | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Uniprot ID | P05413 | Protein name | Fatty acid-binding protein, heart (Fatty acid-binding protein 3) (Heart-type fatty acid-binding protein) (H-FABP) (Mammary-derived growth inhibitor) (MDGI) (Muscle fatty acid-binding protein) (M-FABP) |
| Gene name(s) | FABP3 FABP11 MDGI | Organism | Fatty acid-binding protein, heart (Fatty acid-binding protein 3) (Heart-type fatty acid-binding protein) (H-FABP) (Mammary-derived growth inhibitor) (MDGI) (Muscle fatty acid-binding protein) (M-FABP) |
| Status | reviewed in uniprot database | Length | 133 |
| Mass | 14,858 | Activity regulation | N/A |
| Involvement in disease | N/A | Catalytic activity | N/A |
| PDB ID(s) | 1G5W;1HMR;1HMS;1HMT;2HMB;3RSW;3WBG;3WVM;3WXQ;4TJZ;4TKB;4TKH;4TKJ;4WBK;5B27;5B28;5B29;5CE4;5HZ9;6AQ1;7EGO; |
 |
Function | FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. |
Activity data
 |
Interaction ID | 1033 |
| Target type | Protein | |
| Target unique ID | P05413 | |
| Activity | 0.0743 μM | |
| Binding Conditions/Buffer | 50 mM sodium phosphate buffer, pH 7.4, 50 mM NaCl, 5 mM KCl, 2.5 mM MgCl2 |
|
| Assay | EMSA and CD for specifificity study of aptamer |
|
| PubMed ID | 27545084 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_951 | DNA | CACCTAATACGACTCACTATAGCGGATCCGAGGGGGTAGCGGGTGGGCCGGTGGATGCGGGGCGCCGGCGCCTGGCTCGAACAAGCTTGC | |
| Apta_953 | DNA | TCACCTCATACGACTCACTATAGCGGATCCGAGCCGGGGTGTTCTGTTGGCGGGGGCGGTGGGCGGGCTGGCTCGAACAAGCTTGC | |
| Apta_952 | DNA | TCACCTCATACGACTCACTATAGCGGACCCCACTCACTGCTCGGACTCTCCCCCTCCCCGCCTGGCTCGAACAAGCTTGC | |
| Apta_78 | DNA | ATCTCGACTAGTCATAGGGGGCGCGAACATACGCGGTTGGTGTGGTTGGCTGACAATACTCGT | |
| Apta_589 | DNA | GGGAATTCGAGCTCGGTACCATCTGTGTAAGGGGTAAGGGGTGGGGGTGGGTACGTCTAGCTGCAGGCATGCAAGCTTGG | |
| Apta_589 | DNA | GGGAATTCGAGCTCGGTACCATCTGTGTAAGGGGTAAGGGGTGGGGGTGGGTACGTCTAGCTGCAGGCATGCAAGCTTGG | |
| Apta_281 | DNA | CCGTAATACGACTCACTATAGGGGAGCTCGGTACCGAATTCATGTTGGTAGTTGGCTTGTGTTCGTGTGTTAAGCTTTCAGAGAGGATCCTT | |
| Apta_151 | DNA | GCAATGGTACGGTACTTCCGCGGTCCGGGGTGGGTGGGTGGTGGGGGGTGCGGGGGGGCGGCCGCAAAAGTGCACGCTACTTTGCTAA | |
| Apta_864 | DNA | CTCCTCTGACTGTAACCACGACTAGGACGCTTGGGAGGGGGGGTGGGGTGTCCGGTCGCGGCATAGGTAGTCCAGAAGCC | |
| Apta_878 | DNA | TCGCACATTCCGCTTCTACCGGGGGGGTCGAGCTGAGTGGATGCGAATCTGTGGGTGGGCTTCGCACACACGGACTTAC | |
| Apta_887 | DNA | ACGCTCGGATGCCACTACAGGGGGGTGGTCCTGAGGGTGGTGTGGTTGGTTTGGTTTCCTCATGGACGTGCTGGTGAC | |
| Apta_708 | DNA | CGTACGGAATTCGCTAGCGGGCGGGGGTGCTGGGGGAATGGAGTGCTGCGTGCTGCGGGGATCCGAGCTCCACGTG | |
| Apta_1101 | DNA | CCGTAATACGACTCACTATAGGTATGTGACGCGCGTCAATCGCTGTCCTACCAAGCTTTGCAGAGAGGATCCTT | |
| Apta_581 | DNA | GTACCAGCTTATTCAATTCGCGGAAGACGAATTCCAAGCGCGCGCGGGTCACGCGACTTGGGAATGAGCAAGGGTTGGCCCGAGATAGTATGTTCATCAG | |
| Apta_568 | DNA | CCCAGCAGCACTGGTAGTGAGGCAGTTCACCGGTGGGGCGGTGAGTTTGGCTGCTATTTAT | |
| Apta_656 | DNA | GCACACCGATGGCGGTCCTGTTTTACGGTAGCTTATATGCAGGGGGTGTGCGTGGGGAGGTGCGGTTGC | |
| Apta_771 | DNA | GAATTCCGTCGGACAGCGGTATGCATGGGAGGTTCTGGAGGGGGTGGGTTGGCGTGCGTTCCAGATGGACGAATATCGTCTCCC | |
| Apta_794 | DNA | ATCCAGAGTGACGCAGCAAGGAGATTCGAGGGGGAAGGTTTGTTATAGGGGTTAATGGACACGGTGGCTTAGT | |
| Apta_812 | DNA | ATAGGAGTCACGACGACCAGAATAGAGATATGACAGCGGGGAAGGTTAAGAGGCGCTAGGAGTATGTGCGTCTACCTCTTGACTAAT | |
| Apta_1082 | DNA | GGCTGGTCCGCTGGGAACAAGGGCGGGAGGGAGGGTGTGGGTGCGACAAGCGGACCAGCC |
DEV TOOL
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