Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 356
Aptamer sequence: TTCTCGGTTGGTCTCTGGCGGAGCGATCATCTCAGAGCATTCTTAGCGTTTTGTTCTTGTGTATGATTCGCTTTTCCC
Target unique ID: P19821
Aptamer ID Aptamer descriptor Target chemistry Target name Affinity Binding Conditions/Buffer PubMed ID
Apta_351 TQ 21 Protein DPO1_THEAQ 36 pM 50 mM KCl, 2.5 mM MgCl2, 10 mM Tris-HCl (pH 8.3) 8951376
Structure information of aptamer
Aptamer Sequence: TTCTCGGTTGGTCTCTGGCGGAGCGATCATCTCAGAGCATTCTTAGCGTTTTGTTCTTGTGTATGATTCGCTTTTCCC
The optimal secondary structure in dot-bracket notation: ................((.(((((((((((..(((((((............))))).))...)))).))))))).)).
The centroid secondary structure in dot-bracket notation: ................((.((((((.((((..(((((((............)))))).)...))))..)))))).)).
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
Type Detail Type Detail
Aptamer ID Apta_351 Description TQ 21
Aptamer chemistry DNA Length 78 nt
GC content 47.4% Molecular weight 24,191.21 Da
Molarity of 1 μg/μl solution 41.34 μM Number of G-quadruplexes 2
G-Score 18 Function Targeted binding
Sequence TTCTCGGTTGGTCTCTGGCGGAGCGATCATCTCAGAGCATTCTTAGCGTTTTGTTCTTGTGTATGATTCGCTTTTCCC
Applications Inhibited polymerase activity
Target information
Type Detail Type Detail
Uniprot ID P19821 Protein name DNA polymerase I, thermostable (EC 2.7.7.7) (Taq polymerase 1)
Gene name(s) polA pol1 Organism DNA polymerase I, thermostable (EC 2.7.7.7) (Taq polymerase 1)
Status reviewed in uniprot database Length 832
Mass 93,910 Activity regulation N/A
Involvement in disease N/A Catalytic activity Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1)
PDB ID(s)
1BGX;1JXE;1KTQ;1QSS;1QSY;1QTM;1TAQ;1TAU;2KTQ;3KTQ;3LWL;3LWM;3M8R;3M8S;3OJS;3OJU;3PO4;3PO5;3PY8;3RR7;3RR8;3RRG;3RRH;3RTV;3SV3;3SV4;3SYZ;3SZ2;3T3F;4BWJ;4BWM;4C8K;4C8L;4C8M;4C8N;4C8O;4CCH;4DF4;4DF8;4DFJ;4DFK;4DFM;4DFP;4DLE;4DLG;4ELT;4ELU;4ELV;4KTQ;4N56;4N5S;4XIU;5E41;5KTQ;5NKL;5O7T;5OXJ;5SZT;5W6K;5W6Q;5YTC;5YTD;5YTE;5YTF;5YTG;5YTH;5Z3N;6FBC;6FBD;6FBE;6FBF;6FBG;6FBH;6FBI;6Q4U;6Q4V;
Function N/A
Activity data
Interaction ID 356
Target type Protein
Target unique ID P19821
Activity 36 pM
Binding Conditions/Buffer

50 mM KCl, 2.5 mM MgCl2, 10 mM Tris-HCl (pH 8.3)
Assay

Effect of temperature on inhibition
PubMed ID 8951376
Similar aptamers
Aptamer ID Aptamer chemistry Sequence Similarity
Apta_767 DNA GAATTCAGTCGGACAGCGACGGTGGGAGCCCCAAATAATTCTTGCGATTATTAGTGTAAGCGGATGGACGAATATCGTCTCCC 54.22%
Apta_697 DNA CTCTCGGGACGACGGACGCTAATCTTACAAGGGCGTAGTGTATGTCGTCCC 53.85%
Apta_1181 DNA GAATTCAGTCGGACAGCGTAGTAAGAATCTGTTTCTGGTCCTAGTCTTGCTTGTGTTTCCTGATGGACGAATATCGTCTCCC 53.66%
Apta_770 DNA GAATTCAGTCGGACAGCGGCATTTACATTCCATGGGAAATTTCTCAAGCGGTATTTCATTTCGATGGACGAATATCGTCTCCC 53.01%
Apta_78 DNA ATCTCGACTAGTCATAGGGGGCGCGAACATACGCGGTTGGTGTGGTTGGCTGACAATACTCGT 52.56%
Apta_768 DNA GAATTCAGTCGGACAGCGGCACTTGCGACACGTTTGTCGGGTAAATGCGTGTATTTTCTTTTCGATGGACGAATATCGTCTCCC 52.38%
Apta_933 DNA GACGCTTACTCAGGTGTGACTCGGGGTGTAGCTCGTTATGATTCGGACAAGACTTACCTTGCGCCTCTGGGATCGAAGGACGCAGATGAAGTCTC 51.58%
Apta_626 DNA ATACCAGCTTATTCAATTGGTGGTAGGATGGGCTCGATCGAGGTCTTCAGTCGGCTCGGTTTAGTTTATGTTCTCCTGTAGATAGTAAGTGCAATCT 51.55%
Apta_626 DNA ATACCAGCTTATTCAATTGGTGGTAGGATGGGCTCGATCGAGGTCTTCAGTCGGCTCGGTTTAGTTTATGTTCTCCTGTAGATAGTAAGTGCAATCT 51.55%
Apta_79 DNA ATCTCGAGTCATAGGGGGCGCGAACATACGCGGTTGGTGTGGTTGGCTGACTCGT 51.28%
Apta_357 DNA ACAATGTCGCTCTCCGCCGCAGGAGCATTGTCTGTCTTTATGCTTCTCTTTTTTGTTGC 51.28%
Apta_369 DNA GGCTGTTGTGAGCCTCCTCCCAGAGGGAAGACTTTAGGTTCGGTTCACGTCCCGCTTATTCTTACTCCC 51.28%
Apta_525 DNA ATCCGTCACACCTGCTCTCAATCTGAAATATTGCAAGCAGTGCTCACAATTTGTTGGTGTTGGCTCCCGTAT 51.28%
Apta_611 DNA GCTGCGAGGTGGGTGGGTGGGAGCAATTGGTCCTTGCTTAGCTTCTACGGTGGGCTATCT 51.28%
Apta_726 DNA TACCAGTGCGATGCTCAGGCCGATGTCAACTTTTTCTAACTCACTGGTTTTGCCTGACGCATTCGGTTGAC 51.28%
Apta_737 DNA ATCGTCTGCTCCGTCCAATAGTGCATTGAAACTTCTGCATCCTCGTTTGGTGTGAGGTCGTGC 51.28%
Apta_738 DNA ATCGTCTGCTCCGTCCAATACCTGCATATACACTTTGCATGTGGTTTGGTGTGAGGTCGTGC 51.28%
Apta_1034 DNA ATGCGGATCCCGCGCGCGACCGTGTCAGCGGGGACTAGCGGTGTAGCGCGAAGCTTGCGC 51.28%
Apta_1050 DNA ATCCGTCACACCTGCTCTCACGTCCGTCAACCAGATTGATACTTTATCTTGTTGTGGTGTTGGCTCCCGTAT 51.28%
Apta_771 DNA GAATTCCGTCGGACAGCGGTATGCATGGGAGGTTCTGGAGGGGGTGGGTTGGCGTGCGTTCCAGATGGACGAATATCGTCTCCC 51.19%

ABOUT

AptaDB is an online database we developed to integrate comprehensive information about aptamer.
The detail of this database is described here:

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