AptaDB: Result

Interaction ID: 47

Aptamer sequence: GACCTAGCAGTGGACATGTGGCAGGGTGAAGTGGCATCGTC

Target unique ID: P00432

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_47	CAT 1	Protein	CATA_BOVIN	237 nM	25 mM Tris, 192 mM glycine, 5 mM potassium phosphate buffer (pH 8.3)	22965726

Aptamer Sequence:	GACCTAGCAGTGGACATGTGGCAGGGTGAAGTGGCATCGTC
The optimal secondary structure in dot-bracket notation:	............(((((((.((........)).)))).)))
The centroid secondary structure in dot-bracket notation:	............(((((((.((........)).)))).)))

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_47	Description	CAT 1
Aptamer chemistry	DNA	Length	41 nt
GC content	58.5%	Molecular weight	12,771.19 Da
Molarity of 1 μg/μl solution	78.30 μM	Number of G-quadruplexes	2
G-Score	19	Function	Targeted binding
Sequence	GACCTAGCAGTGGACATGTGGCAGGGTGAAGTGGCATCGTC
Applications	Aptamer exhibited a distinct specificity toward bovine catalase

Type	Detail	Type	Detail
Uniprot ID	P00432	Protein name	Catalase (EC 1.11.1.6)
Gene name(s)	CAT	Organism	Catalase (EC 1.11.1.6)
Status	reviewed in uniprot database	Length	527
Mass	59,915	Activity regulation	N/A
Involvement in disease	N/A	Catalytic activity	Reaction=2 H2O2 = 2 H2O + O2
PDB ID(s)	1TGU;1TH2;1TH3;1TH4;3J7B;3NWL;3RE8;3RGP;3RGS;4BLC;5GKN;6JNT;6JNU;6PM7;6PO0;7CAT;7DI8;8CAT;
Function	Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide.

	Interaction ID	47
	Target type	Protein
	Target unique ID	P00432
	Activity	237 nM
	Binding Conditions/Buffer	25 mM Tris, 192 mM glycine, 5 mM potassium phosphate buffer (pH 8.3)
	Assay	N/A
	PubMed ID	22965726

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_63	DNA	GACCCGTGGTAGGGTAGGATGGGGTGGTC	60.98%
Apta_258	DNA	CACTGCGGGGGTCTATACGTGAGGAAGAAGTGGGCAGGTC	60.98%
Apta_58	DNA	GCACCTTGATGACATGATAGTCGTTGTGTATGCAGTTGGC	58.54%
Apta_666	DNA	TCGACCTCGCGCGAGGAGGGTGGAGGGTCGTAGAGCGCGTA	58.54%
Apta_987	DNA	GTACTGTCAATTGGAAGTGGTGTTACGTTGTGTAGTCAAATCAGTGC	57.45%
Apta_62	DNA	AGTCCGTGGTAGGGCAGGTTGGGGTGACT	56.10%
Apta_65	DNA	CTACCGTGGTAGGGAAGGTTGGAGTGTAG	56.10%
Apta_497	DNA	GTTCGATTGGATTGTGCCGGAAGTGCTGGCTCGAAC	56.10%
Apta_552	DNA	CACCTGGGGGAGTATTGCGGAGGAAGGTTCCAGGTG	56.10%
Apta_578	DNA	CACCAAGCGCAGGGAATTACATTGAAGTGTGGGATTGGCT	56.10%
Apta_590	DNA	ACCATCTGTGTAAGGGGTAAGGGGTGGGGGTGGGTACGTCT	56.10%
Apta_630	DNA	GACCAGGGCAAACGGTAGGGAGTGGTC	56.10%
Apta_1094	DNA	GGAATCGTGGGTGGTAGGGTAGGGGATGCA	56.10%
Apta_1240	DNA	GGAGTAGTGGGGATTGTGGTGTATGTTGTC	56.10%
Apta_1256	DNA	GATCGGGTGTGGGTGGCGTAAAGGGAGCATCGGACA	56.10%
Apta_752	DNA	GCTATCTTATGGAAATTTCGTGTAGGGTTTGGTGTGGCGGGGCTA	55.56%
Apta_585	DNA	GATCGAGGGCAGCGATAGCTGGGCTAATAAGGTTAGCCCCATCGGTC	55.32%
Apta_939	DNA	TTGCCCACCGTACTGTGCAGGTCGAACTACAGGCACGTCGTG	54.76%
Apta_652	DNA	CCTGCACCTCGTCCAGCATGCACACCGATGGCGGTCCTGGCAGGGGGTGTGCGTG	54.55%
Apta_41	DNA	CGGTCGCTCCGTGTGGCTTGGGTTGGGTGTGGCAGTGAC	53.66%

ABOUT