AptaDB: Result

Interaction ID: 497

Aptamer sequence: GCGAGGTGTTCGAGAGTTAGGGGCGACATGACCAAACGTT

Target unique ID: Q8AY56

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_492	clone 24	Protein	3LKA_BUNCA	7.5 μM	137 mM NaCl, 2.7 mM KCl, 4.2 mM Na2HPO4, and 0.005% Tween 20	22860007

Aptamer Sequence:	GCGAGGTGTTCGAGAGTTAGGGGCGACATGACCAAACGTT
The optimal secondary structure in dot-bracket notation:	(((.(((..(((...........)))....)))...))).
The centroid secondary structure in dot-bracket notation:	........................................

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_492	Description	clone 24
Aptamer chemistry	DNA	Length	40 nt
GC content	55.0%	Molecular weight	12,466.01 Da
Molarity of 1 μg/μl solution	80.22 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Targeted therapy
Sequence	GCGAGGTGTTCGAGAGTTAGGGGCGACATGACCAAACGTT
Applications	Against α-bungarotoxin

Type	Detail	Type	Detail
Uniprot ID	Q8AY56	Protein name	Kappa 1a-bungarotoxin
Gene name(s)	N/A	Organism	Kappa 1a-bungarotoxin
Status	reviewed in uniprot database	Length	87
Mass	9,580	Activity regulation	N/A
Involvement in disease	N/A	Catalytic activity	N/A
PDB ID(s)	N/A
Function	Postsynaptic neurotoxin that binds and inhibits neuronal nicotinic acetylcholine receptors (nAChR) with high affinity.

	Interaction ID	497
	Target type	Protein
	Target unique ID	Q8AY56
	Activity	7.5 μM
	Binding Conditions/Buffer	137 mM NaCl, 2.7 mM KCl, 4.2 mM Na2HPO4, and 0.005% Tween 20
	Assay	Binding specificity of the aptamer obtained from clone 24 & 51 by surface plasmon resonance (SPR) technique using Biacore 3000
	PubMed ID	22860007

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_485	DNA	TGTCGTTGGGGGGGTAGGGAAGGGTGAAACGACAAGCCAGAAGTT	55.56%
Apta_46	DNA	CGGGTGGGAGTTGTGGGGGGGGGTGGGAGGGTT	55.00%
Apta_259	DNA	GCAAGTATGAGCGCAGGAGTTAGGTCCCGTGGCGATGGGT	55.00%
Apta_579	DNA	ACTGAGGGCACGGACAGGAGGGGGAGAGATGGCGTGAGGT	55.00%
Apta_850	DNA	CACCAGTGTGTTGAGGTTTGAGCACACTGATAGAGTGTCA	55.00%
Apta_1026	DNA	GGGGGGTGTATCGTTGACGAGTTGCGCGTGCGTCTCGTG	55.00%
Apta_991	DNA	AGCAGTGAAGAGGCGAGAGATTTATGAGGGTAGCGTCCACATATGCCAGGATCGTGTTG	54.24%
Apta_1043	DNA	TTCAAGGCGGCGAGATTTAGTGGTTGGGAGGCTGTACGCCCTACGTGAAC	54.00%
Apta_754	DNA	GGGCGGGGAGTAGGGAGAGGGGTTTCCATCGGCGACAGAGGAGTTATGTGTGT	52.83%
Apta_491	DNA	TTGGGGTCTGCTCGGGATTGCGGAGAACGTGAATCT	52.50%
Apta_671	DNA	GCATCTGATCGGGTGTGGGTGGCGTAAAGG	52.50%
Apta_959	DNA	GGACCGCTAGGTTCGTGGCTGAGATCACGCGATATCAGTA	52.50%
Apta_1138	DNA	GCAGTGTGGGCGAATCTATGCGTACCGTTCGATATCGTG	52.50%
Apta_861	DNA	CAGGTTGGTCTGGTTGGGTTTGGCTCCTGTGTACGCAACCTG	52.38%
Apta_341	DNA	TCGGGGTTTGGGGGTTCGGGGTCGGTTCGGTTTCTTCTAATGGA	52.27%
Apta_596	DNA	GGTGGCTGTTGGTCGAGGCTTATTAGTGTAGTCAATGGAGGCAGGCGACG	52.00%
Apta_882	DNA	AATAGTCTGGTTCGGTGCTTGGTGCGATGTGTTAGTCAGACTCTTGTGTG	52.00%
Apta_941	DNA	CGGGTGCCTTAAAGTGTAGGTGGGAGGGGGATGATTATGTACGATCAAGT	52.00%
Apta_753	DNA	GGGCACAGACGGAAGATGAGAATTGTGGGGCTTAGTATAGTGAGGTGCGTGT	51.92%
Apta_600	DNA	GTCTCTGTGTGCGCCAGAGAACACTGGGGCAGATATGGGCCAGCACAGAATGAGGCCC	51.72%

ABOUT