AptaDB: Result

Interaction ID: 32

Aptamer sequence: CGGTAAACTGCGTCCGAAGGGGCTGCAGTGACCCGAATGGGTCCG

Target unique ID: O14786

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_32	aptamer 1	Protein	NRP1_HUMAN	3.8 pM	1 mM KH2PO4, 3 mM Na2HPO4 and 155 mM NaCl, pH 7.4, Gibco	27387284

Aptamer Sequence:	CGGTAAACTGCGTCCGAAGGGGCTGCAGTGACCCGAATGGGTCCG
The optimal secondary structure in dot-bracket notation:	......(((((((((....)))).)))))(((((....)))))..
The centroid secondary structure in dot-bracket notation:	......(((((((((....)))).)))))(((((....)))))..

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_32	Description	aptamer 1
Aptamer chemistry	DNA	Length	45 nt
GC content	64.4%	Molecular weight	13,952.93 Da
Molarity of 1 μg/μl solution	71.67 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Targeted binding
Sequence	CGGTAAACTGCGTCCGAAGGGGCTGCAGTGACCCGAATGGGTCCG
Applications	Biological experiments using cultured cells revealed that our stabilized aptamers efficiently inhibited the interaction between VEGF165 and its receptor, with the same or slightly higher efficiency than that of the pegaptanib RNA aptamer

Type	Detail	Type	Detail
Uniprot ID	O14786	Protein name	Neuropilin-1 (Vascular endothelial cell growth factor 165 receptor) (CD antigen CD304)
Gene name(s)	NRP1 NRP VEGF165R	Organism	Neuropilin-1 (Vascular endothelial cell growth factor 165 receptor) (CD antigen CD304)
Status	reviewed in uniprot database	Length	923
Mass	103,134	Activity regulation	N/A
Involvement in disease	N/A	Catalytic activity	N/A
PDB ID(s)	1KEX;2QQI;2QQM;2QQN;3I97;4DEQ;4RN5;5C7G;5IJR;5IYY;5J1X;5JGI;5JGQ;5JHK;5L73;6FMC;6FMF;6TKK;7JJC;7O1N;
Function	Cell-surface receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system.

	Interaction ID	32
	Target type	Protein
	Target unique ID	O14786
	Activity	3.8 pM
	Binding Conditions/Buffer	1 mM KH2PO4, 3 mM Na2HPO4 and 155 mM NaCl, pH 7.4, Gibco
	Assay	Binding analysis of anti-VEGF165 aptamers by a BIAcore T200 at 25 ℃ or 37 ℃
	PubMed ID	27387284

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_33	DNA	GCGGTAAACTGCGTCCGAAGGGGCTGCAGTGACCCGAATGGGTCCGCCGCGAAGCG	80.36%
Apta_146	DNA	GGTGCGGTTCGTGCGGTTGTAGTACTCGTGGCCG	60.00%
Apta_306	DNA	CAAGGTAACCAGTACAAGGTGCTAAACGTAATGGCTTCG	57.78%
Apta_1068	DNA	CGGGACAAGACAACGAACGAACAGGAAGAGAACCGGAATGCAGACGTCAGGG	55.77%
Apta_148	DNA	CCGTAGGTTCGGGGTCGGAGTGGTCCGGAAGGTGGCGTGG	55.56%
Apta_149	DNA	CCGTAGGTTCGGGGTCGGAGTGGTCCGGAAGATGGCGTGG	55.56%
Apta_150	DNA	CCGTAGGTTCGGGGCGGAGTGGTCCGGAAGGTGGCGTGG	55.56%
Apta_498	DNA	GAACTCATTGGCTGGAGGCGGCAGTACCGCTTGAGTT	55.56%
Apta_1022	DNA	CTCCTTCAACGAAGTGGGTTCCTTCAACGAAGTGGGTCTC	55.56%
Apta_1023	DNA	CTCCTTCAACGAAGTGGGTCTCCTTCAACGAAGTGGGTCTC	55.56%
Apta_1126	DNA	TCGGGCGAGTCGTCTGCGGGCGGATAGACTACGACACCGCATCGTCCTCCC	54.90%
Apta_288	DNA	ATCCAGAGTGACGCAGCATTTCATCGGGTCCAAAAGGGGCTGCTCGGGATTGCGGATATGGACACGT	53.73%
Apta_162	DNA	CCGTACATACTACGTACGTACGTGAACAGAGGCAGAG	53.33%
Apta_255	DNA	TAGCGATACTGCGTGGGTTGGGGCGGGTAGGGCCAGCAGTCTCGT	53.33%
Apta_677	DNA	CCAAATGCGACGGGGCCTGTTTTAATGGGG	53.33%
Apta_686	DNA	GGTCACCAACAACAGGGAGCGCTACGCGAAGGGTCAATGTGACGTCATGCGGATGTGTGG	53.33%
Apta_693	DNA	CGAGTTGAGCCGGGCGCGGTACGGGTACTGGTATGTGTGG	53.33%
Apta_870	DNA	CCGGGGAATCTAGGGGAGGGCGGGTGGGTCAATTGAGCCG	53.33%
Apta_1034	DNA	ATGCGGATCCCGCGCGCGACCGTGTCAGCGGGGACTAGCGGTGTAGCGCGAAGCTTGCGC	53.33%
Apta_1035	DNA	ATGCGGATCCCGCGCGGGTCTGAGGAGTGCGCGGTGCCAGTGAGTGCGCGAAGCTTGCGC	53.33%

ABOUT