AptaDB: Result

Interaction ID: 490

Aptamer sequence: TGTCGTTGGGGGGGTAGGGAAGGGTGAAACGACAAGCCAGAAGTT

Target unique ID: Q7AP54

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_485	B2	Protein	HBP2_LISMO	73 nM	20 mM Tris-AcOH (pH 8.0), 100 mM NaCl, 200 mM KCl, 5 mM MgCl2, 0.5% Triton X-100, and 5% DMSO	22954361

Aptamer Sequence:	TGTCGTTGGGGGGGTAGGGAAGGGTGAAACGACAAGCCAGAAGTT
The optimal secondary structure in dot-bracket notation:	(((((((....................)))))))...........
The centroid secondary structure in dot-bracket notation:	(((((((....................)))))))...........

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_485	Description	B2
Aptamer chemistry	DNA	Length	45 nt
GC content	55.6%	Molecular weight	14,160.09 Da
Molarity of 1 μg/μl solution	70.62 μM	Number of G-quadruplexes	3
G-Score	63	Function	Aptasensor
Sequence	TGTCGTTGGGGGGGTAGGGAAGGGTGAAACGACAAGCCAGAAGTT
Applications	Peroxidase activity and strong binding affinity to hemin

Type	Detail	Type	Detail
Uniprot ID	Q7AP54	Protein name	Hemin/hemoglobin-binding protein 2 (Hn/Hb-binding protein 2) (P64) (Surface virulence-associated protein A)
Gene name(s)	hbp2 svpA lmo2185	Organism	Hemin/hemoglobin-binding protein 2 (Hn/Hb-binding protein 2) (P64) (Surface virulence-associated protein A)
Status	reviewed in uniprot database	Length	569
Mass	63,380	Activity regulation	Is overexpressed in mecA, clpC and clpP mutants
Involvement in disease	N/A	Catalytic activity	N/A
PDB ID(s)	4MYP;4NLA;
Function	Acts as an extracellular and cell wall-bound hemophore; scavenges host heme and hemoglobin from the environment and also serves as a cell wall receptor for both.

	Interaction ID	490
	Target type	Protein
	Target unique ID	Q7AP54
	Activity	73 nM
	Binding Conditions/Buffer	20 mM Tris-AcOH (pH 8.0), 100 mM NaCl, 200 mM KCl, 5 mM MgCl2, 0.5% Triton X-100, and 5% DMSO
	Assay	CD spectra of selected sequences (4 μM) in PB buffer without (black line) and with (red line) K+
	PubMed ID	22954361

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_1082	DNA	GGCTGGTCCGCTGGGAACAAGGGCGGGAGGGAGGGTGTGGGTGCGACAAGCGGACCAGCC	56.67%
Apta_572	DNA	CCGTAACCCTGCGGGGGGGGGAGAAGGCAATGGGGGACAACTCGCCGGTAGCCATCCATAT	55.74%
Apta_375	DNA	TTGGGGAGGGGCACTGGGCAGTGTAATTTACGAAAGCGAG	55.56%
Apta_492	DNA	GCGAGGTGTTCGAGAGTTAGGGGCGACATGACCAAACGTT	55.56%
Apta_746	DNA	AGAGTGGGGGGGTGGGTGGATTTGACAGGTGGCATGCTGGAGAGT	55.56%
Apta_279	DNA	TTTTTTTTGGGCGAAGACCGGGACGGGAGGAAAGAGATGTTTGGTTTT	54.17%
Apta_940	DNA	TGTGCGGGGGAGGGGAGTGGCGCTGTTGGTGCGGGTATAGCGCGTGGTGT	54.00%
Apta_943	DNA	GAAGGTGGAGCGGTTAGGATACTAGCGAATGAAGACACGTTAGCTGATCT	54.00%
Apta_50	DNA	AGTGGCGAGGGGGGTAGGTAAAGTATCCTTAGTGTGTTG	53.33%
Apta_565	DNA	GCGGGGTTGGGCGGGTGGGTTCGCTGGGCAGGGGGCGAGTG	53.33%
Apta_1014	DNA	TGGGGGTTGGGTGGATAGGCTGGCGTCGGGGCAGGTCAGT	53.33%
Apta_1093	DNA	AGGAGGGTAGGTAGTGCTTGGTAGGGAAACTCCGCCGATT	53.33%
Apta_1045	DNA	GGTGCTCAGGGAACTGTCTGAGGGATCAGGCTTAAGCCTGTCGAGCAGTT	52.00%
Apta_569	DNA	TGCGCATGTGAAAGGTTGCCTAACTGGACAGGGTTTAGGAGCGACTAGACACAG	51.85%
Apta_1213	DNA	GGACTTGAAGAAAAGGGCGACACGCGAAGTCGATGTCGCGTCTGCCTGAAGTCA	51.85%
Apta_607	DNA	TGAGGCGGGTGGGTGGGTTGAATATGCTGATTACCCCATCGGAGAACGTTAAGGCGCTTC	51.67%
Apta_608	DNA	TGAGGCGGGTGGGTGGGTTGAATACGCTGATCACCCCATCGGAGAACGTTAAGGCGCTTT	51.67%
Apta_686	DNA	GGTCACCAACAACAGGGAGCGCTACGCGAAGGGTCAATGTGACGTCATGCGGATGTGTGG	51.67%
Apta_3	DNA	CCTGCCTTTGGCAGCCGGGCGAAGGCAACCCGACACCGACAGC	51.11%
Apta_46	DNA	CGGGTGGGAGTTGTGGGGGGGGGTGGGAGGGTT	51.11%

ABOUT