Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 480
Aptamer sequence: CAAAAAGATAGGTGGATTTTTCCCCGGGTCGGTTTGTATG
Target unique ID: Q61727
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_475 | R5C2 | Protein | I10R1_MOUSE | 55 nM | 150 mmol/L NaCl, 20 mmol/L KCl, and 2 mmol/L HEPES heated to 82 °C and slowly cooling to room temperature | 22434135 |
Structure information of aptamer
| Aptamer Sequence: | CAAAAAGATAGGTGGATTTTTCCCCGGGTCGGTTTGTATG |
| The optimal secondary structure in dot-bracket notation: | ((((..(((.((.(((....)))))..)))..)))).... |
| The centroid secondary structure in dot-bracket notation: | ((((..(((.((.(((....)))))..)))..)))).... |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_475 | Description | R5C2 |
| Aptamer chemistry | DNA | Length | 40 nt |
| GC content | 45.0% | Molecular weight | 12,396.97 Da |
| Molarity of 1 μg/μl solution | 80.66 μM | Number of G-quadruplexes | No QGRS found |
| G-Score | N/A | Function | Targeted therapy |
| Sequence | CAAAAAGATAGGTGGATTTTTCCCCGGGTCGGTTTGTATG | ||
| Applications | Bound to IL-10 receptor on the cell surface and blocked IL-10 function in vitro | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Uniprot ID | Q61727 | Protein name | Interleukin-10 receptor subunit alpha (IL-10 receptor subunit alpha) (IL-10R subunit alpha) (IL-10RA) (CDw210a) (Interleukin-10 receptor subunit 1) (IL-10R subunit 1) (IL-10R1) (CD antigen CD210) |
| Gene name(s) | Il10ra Il10r | Organism | Interleukin-10 receptor subunit alpha (IL-10 receptor subunit alpha) (IL-10R subunit alpha) (IL-10RA) (CDw210a) (Interleukin-10 receptor subunit 1) (IL-10R subunit 1) (IL-10R1) (CD antigen CD210) |
| Status | reviewed in uniprot database | Length | 575 |
| Mass | 64,248 | Activity regulation | N/A |
| Involvement in disease | N/A | Catalytic activity | N/A |
| PDB ID(s) | N/A |
 |
Function | Cell surface receptor for the cytokine IL10 that participates in IL10-mediated anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. |
Activity data
|
No relevant experimental diagram |
Interaction ID | 480 |
| Target type | Protein | |
| Target unique ID | Q61727 | |
| Activity | 55 nM | |
| Binding Conditions/Buffer | 150 mmol/L NaCl, 20 mmol/L KCl, and 2 mmol/L HEPES heated to 82 °C and slowly cooling to room temperature |
|
| Assay | N/A |
|
| PubMed ID | 22434135 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_474 | DNA | CAAAAAGATAGGTGGATTTTTCCCCGGATCGGTTTGTATG | |
| Apta_476 | DNA | CCTATCTTATTCGTTGCGGACTCCCCGGTCGGTATGTATG | |
| Apta_477 | DNA | CGCGGAAGAATTGCTATGTGTGCCCCGGTTGGTCTGTATA | |
| Apta_478 | DNA | CTTCACTTTCGTGGACGGTGGCGTCGGGGCGGTTTGTATG | |
| Apta_344 | DNA | CACTACAGAGGTTGCGTCTGTCCCACGTTGTCATGGGGGGTTGGCCTG | |
| Apta_484 | DNA | CAGGGAGGGACAGGGCGGGAATTTGTATGATGGTTCGTTTGCTCG | |
| Apta_649 | DNA | GCACACCGATGGCGGTCCTGTTTAGGGGGTGTGCGTG | |
| Apta_681 | DNA | CCTGACGATCGGGTGTGGGTTGGCTTGAGG | |
| Apta_683 | DNA | GCAGTACGATCGGGGGTGGGTGGATGTAGG | |
| Apta_1149 | DNA | ATCATGGTGGGTATCGGCACTCGTTGGTTGAT | |
| Apta_1087 | DNA | CTTACGACTCAGGCATTTTGCCGGGTAACGAAGTTACTGTCGTAAG | |
| Apta_694 | DNA | ACTCACTATGGAAGAGATGGCGACATCTCTTCTCCGAGCCGGTCGAAATAGTGAGT | |
| Apta_1092 | DNA | CCCATGGTAGGTATTGCTTGGTAGGGATAGTGGGCTTGATG | |
| Apta_750 | DNA | ATGCGAACAGGTGGGTGGGTTGGGTGGATTGTTCGGCTTCTTGAT | |
| Apta_754 | DNA | GGGCGGGGAGTAGGGAGAGGGGTTTCCATCGGCGACAGAGGAGTTATGTGTGT | |
| Apta_392 | DNA | GGATAGTTCGGTGGCTGTTCAGGGTCTCCTCCT | |
| Apta_411 | DNA | AGACGTAAGTTAATTGGACTTGGTCGTGTGCGGCACAGCGATTGAAAT | |
| Apta_480 | DNA | CGGCACACCTCGGTCGGTTTGTATGGCGAGCTGGTTTTTT | |
| Apta_578 | DNA | CACCAAGCGCAGGGAATTACATTGAAGTGTGGGATTGGCT | |
| Apta_674 | DNA | GCACGATGGGGAAAGGGTCCCCCTGGGTTG |
DEV TOOL
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