AptaDB: Result

Interaction ID: 482

Aptamer sequence: CGCGGAAGAATTGCTATGTGTGCCCCGGTTGGTCTGTATA

Target unique ID: Q61727

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_477	R5Cw2	Protein	I10R1_MOUSE	130 nM	150 mmol/L NaCl, 20 mmol/L KCl, and 2 mmol/L HEPES heated to 82 °C and slowly cooling to room temperature	22434135

Aptamer Sequence:	CGCGGAAGAATTGCTATGTGTGCCCCGGTTGGTCTGTATA
The optimal secondary structure in dot-bracket notation:	.(((((..((((((.......))...))))..)))))...
The centroid secondary structure in dot-bracket notation:	.(((((..((((((.......))...))))..)))))...

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_477	Description	R5Cw2
Aptamer chemistry	DNA	Length	40 nt
GC content	52.5%	Molecular weight	12,373.93 Da
Molarity of 1 μg/μl solution	80.82 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Targeted therapy
Sequence	CGCGGAAGAATTGCTATGTGTGCCCCGGTTGGTCTGTATA
Applications	Bound to IL-10 receptor on the cell surface and blocked IL-10 function in vitro

Type	Detail	Type	Detail
Uniprot ID	Q61727	Protein name	Interleukin-10 receptor subunit alpha (IL-10 receptor subunit alpha) (IL-10R subunit alpha) (IL-10RA) (CDw210a) (Interleukin-10 receptor subunit 1) (IL-10R subunit 1) (IL-10R1) (CD antigen CD210)
Gene name(s)	Il10ra Il10r	Organism	Interleukin-10 receptor subunit alpha (IL-10 receptor subunit alpha) (IL-10R subunit alpha) (IL-10RA) (CDw210a) (Interleukin-10 receptor subunit 1) (IL-10R subunit 1) (IL-10R1) (CD antigen CD210)
Status	reviewed in uniprot database	Length	575
Mass	64,248	Activity regulation	N/A
Involvement in disease	N/A	Catalytic activity	N/A
PDB ID(s)	N/A
Function	Cell surface receptor for the cytokine IL10 that participates in IL10-mediated anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation.

No relevant experimental diagram	Interaction ID	482
	Target type	Protein
	Target unique ID	Q61727
	Activity	130 nM
	Binding Conditions/Buffer	150 mmol/L NaCl, 20 mmol/L KCl, and 2 mmol/L HEPES heated to 82 °C and slowly cooling to room temperature
	Assay	N/A
	PubMed ID	22434135

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_474	DNA	CAAAAAGATAGGTGGATTTTTCCCCGGATCGGTTTGTATG	60.00%
Apta_475	DNA	CAAAAAGATAGGTGGATTTTTCCCCGGGTCGGTTTGTATG	60.00%
Apta_1257	DNA	CGACGCACAGTCGCTGGTGCGTACCTGGTTGCCGTTGTGT	57.50%
Apta_476	DNA	CCTATCTTATTCGTTGCGGACTCCCCGGTCGGTATGTATG	55.00%
Apta_681	DNA	CCTGACGATCGGGTGTGGGTTGGCTTGAGG	55.00%
Apta_1032	DNA	TGGCAAGAGGGTGTGCTTAAGGTGGACACGGTGGCTTAG	55.00%
Apta_1149	DNA	ATCATGGTGGGTATCGGCACTCGTTGGTTGAT	55.00%
Apta_1271	DNA	ACCACAGCCAGTAAGTCCCGTAGGCCCTGGTTGGGCTGTT	55.00%
Apta_411	DNA	AGACGTAAGTTAATTGGACTTGGTCGTGTGCGGCACAGCGATTGAAAT	54.17%
Apta_1092	DNA	CCCATGGTAGGTATTGCTTGGTAGGGATAGTGGGCTTGATG	53.66%
Apta_571	DNA	ACCTGCGATAGGTAGTTTGTTTCTGTTGCCCTAGCGTCCGTCGTAACAAGTCA	52.83%
Apta_46	DNA	CGGGTGGGAGTTGTGGGGGGGGGTGGGAGGGTT	52.50%
Apta_57	DNA	GCGGGTCGGTTGCTCGCTTCGCCCGATCGGTCTAAGGGTG	52.50%
Apta_69	DNA	AGCGAGTAGGTTGGTGTGGTTGGGGCTCGCT	52.50%
Apta_74	DNA	GGACAGTAGGTTGGTGTGGTTGGGGCTGTCC	52.50%
Apta_163	DNA	GCGCATGACCATTGATGTCTGCACCATCAGAGGCAGAG	52.50%
Apta_215	DNA	AGGAAGGCTTTAGGTCCGAGATCTCGGAAT	52.50%
Apta_260	DNA	GACGTTAAGCTAGCAGGTGTTAGGTCCCGTGGTGATGAAT	52.50%
Apta_682	DNA	CCTTGTAGATCGGGTGTGGTTTGGCGTAGG	52.50%
Apta_1085	DNA	TGGGGTGGTGGTGTGGGTGGGGGTTTGTTC	52.50%

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