AptaDB: Result

Interaction ID: 1216

Aptamer sequence: TTCTGGTGTGGTAACTCATGTATCGGTCGTGGGGGTCGTGGCATT

Target unique ID: P03524

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_1156	GT1	Protein	GLYCO_RABVE	395 nM	4.5 g/l glucose and 5 mmol/l MgCl2 in Dulbecco’s PBS	24486485

Aptamer Sequence:	TTCTGGTGTGGTAACTCATGTATCGGTCGTGGGGGTCGTGGCATT
The optimal secondary structure in dot-bracket notation:	.....((((.....((((((.......)))))).......)))).
The centroid secondary structure in dot-bracket notation:	.....((((.....((((((.......)))))).......)))).

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_1156	Description	GT1
Aptamer chemistry	DNA	Length	45 nt
GC content	53.3%	Molecular weight	13,991.93 Da
Molarity of 1 μg/μl solution	71.47 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Targeted therapy
Sequence	TTCTGGTGTGGTAACTCATGTATCGGTCGTGGGGGTCGTGGCATT
Applications	Inhibit the replication of RABV

Type	Detail	Type	Detail
Uniprot ID	P03524	Protein name	Glycoprotein
Gene name(s)	G	Organism	Glycoprotein
Status	reviewed in uniprot database	Length	524
Mass	58,658	Activity regulation	N/A
Involvement in disease	N/A	Catalytic activity	N/A
PDB ID(s)	3NFK;
Function	Attaches the virus to host cellular receptor, inducing endocytosis of the virion. In the endosome, the acidic pH induces conformational changes in the glycoprotein trimer, which trigger fusion between virus and cell membrane.

	Interaction ID	1216
	Target type	Protein
	Target unique ID	P03524
	Activity	395 nM
	Binding Conditions/Buffer	4.5 g/l glucose and 5 mmol/l MgCl2 in Dulbecco’s PBS
	Assay	The inhibition of non-RABV viral infections by aptamers in vitro
	PubMed ID	24486485

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_1190	DNA	TTCAGGGCGGGGTAAACGGGAGGTGGGGGGGGCTTGGGAC	60.00%
Apta_266	DNA	TGGATGGTAGCTCGGTCGGGGTGGGTGGGTTGGCAAGTCT	57.78%
Apta_364	DNA	TATGGTATGCTGTGTGGTATGGGGTGGCGTGCTCT	57.78%
Apta_1149	DNA	ATCATGGTGGGTATCGGCACTCGTTGGTTGAT	57.78%
Apta_46	DNA	CGGGTGGGAGTTGTGGGGGGGGGTGGGAGGGTT	55.56%
Apta_146	DNA	GGTGCGGTTCGTGCGGTTGTAGTACTCGTGGCCG	55.56%
Apta_160	DNA	CGGTGCAGTGGATACATGCCAGCCGTAGCCATCGTGGATA	55.56%
Apta_264	DNA	GGCGTTGTCGGGCGCAGGTGTAGGCCTCGTGGTGGTGGGT	55.56%
Apta_752	DNA	GCTATCTTATGGAAATTTCGTGTAGGGTTTGGTGTGGCGGGGCTA	55.56%
Apta_1014	DNA	TGGGGGTTGGGTGGATAGGCTGGCGTCGGGGCAGGTCAGT	55.56%
Apta_344	DNA	CACTACAGAGGTTGCGTCTGTCCCACGTTGTCATGGGGGGTTGGCCTG	54.17%
Apta_463	DNA	GTTCTCATGCACACTTATAGCGGGGGGATCCAGTACT	53.33%
Apta_491	DNA	TTGGGGTCTGCTCGGGATTGCGGAGAACGTGAATCT	53.33%
Apta_493	DNA	GGGGTGGTGGGTTCTTTTTGTGGTATTGGTGTGGGGGGCA	53.33%
Apta_497	DNA	GTTCGATTGGATTGTGCCGGAAGTGCTGGCTCGAAC	53.33%
Apta_577	DNA	ACTTCAGTGAGTTGTCCCACGGTCGGCGAGTCGGTGGTAG	53.33%
Apta_590	DNA	ACCATCTGTGTAAGGGGTAAGGGGTGGGGGTGGGTACGTCT	53.33%
Apta_646	DNA	TGGAGCTTGGATTGATGTGGTGTGTGAGTGCGGTGCCC	53.33%
Apta_679	DNA	CAGGTGGCAGATCGGGTGTGGGTGGCCTGG	53.33%
Apta_744	DNA	GTGGCCAGTCACTCAATTGGGTGTAGGGGTGGGGATTGTGGGTTG	53.33%

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