Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 6
Aptamer sequence: GGAAAGACGCUAGCGAAUUGGUUCCUCGAAAGGGGAAAGCGUUAUUAAGAAACCAAAAUUUCC
Target unique ID: G3GC05
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_6 | Family II-Truncated | Protein | G3GC05_BEMTA | N/A | 10 mM HEPES (pH 7.5) and 10 mM MgCl2 | 11094337 |
Structure information of aptamer
| Aptamer Sequence: | GGAAAGACGCUAGCGAAUUGGUUCCUCGAAAGGGGAAAGCGUUAUUAAGAAACCAAAAUUUCC |
| The optimal secondary structure in dot-bracket notation: | (((((((((((..........((((((....))))))))))))...............))))) |
| The centroid secondary structure in dot-bracket notation: | .....((((((..........((((((....)))))))))))).................... |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_6 | Description | Family II-Truncated |
| Aptamer chemistry | RNA | Length | 63 nt |
| GC content | 42.9% | Molecular weight | 20,370.21 Da |
| Molarity of 1 μg/μl solution | 49.09 μM | Number of G-quadruplexes | No QGRS found |
| G-Score | N/A | Function | Targeted binding |
| Sequence | GGAAAGACGCUAGCGAAUUGGUUCCUCGAAAGGGGAAAGCGUUAUUAAGAAACCAAAAUUUCC | ||
| Applications | Regulated kinase 2 (ERK2) | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Uniprot ID | G3GC05 | Protein name | Mitogen-activated protein kinase (EC 2.7.11.24) |
| Gene name(s) | Erk1/2 | Organism | Mitogen-activated protein kinase (EC 2.7.11.24) |
| Status | unreviewed in uniprot database | Length | 363 |
| Mass | 41,910 | Activity regulation | Activated by threonine and tyrosine phosphorylation |
| Involvement in disease | N/A | Catalytic activity | Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein] |
| PDB ID(s) | N/A |
 |
Function | N/A |
Activity data
 |
Interaction ID | 6 |
| Target type | Protein | |
| Target unique ID | G3GC05 | |
| Activity | N/A | |
| Binding Conditions/Buffer | 10 mM HEPES (pH 7.5) and 10 mM MgCl2 |
|
| Assay | Dissociation constants of family II RNA. Trace, 5P-end labeled family II RNA was incubated with diphosphorylated or unphosphorylated ERK2 over a protein concentration range from 2 WM to 5.6 pM. RNA-protein complexes were captured on nitrocellulose membranes and data were analyzed as described |
|
| PubMed ID | 11094337 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_382 | RNA | GGCGUGACCUGAUGAGUCACGCAGACGCUAGCGAAUUGGUUCCUCGAAAGGGGAAAGCGUUAUUAAGAAACCAAAAUGUGUUACGAAACGUUCCC | |
| Apta_225 | RNA | GGGGCCACCAACGACAUUUAUCGAAUUGAUAACCUUACGCGAGAGCGUAGUUCGUUGAUAUAAAUAGUGCCCAUGGAUC | |
| Apta_612 | RNA | GGGCGACCCUGAUGAGCCUUAGGAUAUGCUUCGGCAGAAGGACGUCGAAACGGUGAAAGCCGUAGGUUGCCC | |
| Apta_388 | RNA | GCGGAAGCGUGCUGGGCUAGAAUAAUAAUAAGAAAACCAGUACUUUCGU | |
| Apta_460 | RNA | GGAUGGCAGCGGAGAGUUGCUUGGAAUGCGUUAUAGUCUCUUAGGUGUGUACAGCCCACACCACUCUCC | |
| Apta_383 | RNA | GGCGUGACCUGAUGAGUCACGCUAAGGAGGAUUUCCGAAAGCGGCUACGGUCCGCCAGUGUUACGAAACGUUCCC | |
| Apta_398 | RNA | GGGAGGACGAUGCGGCUUUAAUCCUCGCACUCAGCGCGCAUCACCCUUGACAUCACAGACGACUCGCUGAGGAUCCGAGA | |
| Apta_723 | RNA | GGGAGCUCAGAAUAAACGCUCAAAUCCGUGGACAGGGCGUAAGCGCCUUCGACAUGAGACACGGAUCCUGCGACGAAUUCAGC | |
| Apta_886 | RNA | GGGAGACGAUAUUCGUCCAUUCGGGUGGCCCGUGUCUGAGCGGGGACGGCCACUUGAGCGCCGCUGUCCGACUGAAUUCUCGACC | |
| Apta_724 | RNA | GGGAGAGGAUACUACUGUCAUACGUUAGGCUGUAGGCGAGGUGAAAUGAGCGGUAAUAGCCUCAGCGUAGCAUAUGCAUGAAUUCGAAGCUUCGC | |
| Apta_5 | RNA | GGGAGAGCCAUACCUGACAAAGACGCUAGCGAAUUGGUUCCUCACUCAAAAGUAGGGGAAAGCGUUAUUAAGAAACCAAAAUUUGACAGGUUACGCAUCCUGCAUCCUCCAGGUUACGCAUCC | |
| Apta_305 | RNA | GGGAGGACGAUGCGGAUCGCCCUGAACCGGCCCAGCAGACUGCUGACGGCACGAUCCGCAUCGUCCUCCC | |
| Apta_280 | RNA | GAUAAUACGACUCACUAUAGGGUUCACUGCAGACUUGACGAAGCUUGCAGAAAAGGGGGAAGAAGAGGGUGAUUCAGGCGAGAGAAUGGAUCCACAUCUACGAAUUC | |
| Apta_549 | RNA | GGAGCUCAGCCUUCACUGCAUGAUAAACCGAUGCUGGGCGAUUCUCCUGAAGUAGGGGAAGAGUUGUCAUGUAUGGGGGCACCACGGUCGGAUCCUG | |
| Apta_171 | RNA | GGGAGCUCAGAAUAAACGCUCAACCAGUCUUGUGGCUUUGAAAGAGAGGAGUGUUCGACAUGAGGCCCGGAUCCGGC | |
| Apta_130 | RNA | GGGAGGACGAUGCGGUGGUAGCGCGAUAUAGCGCUGGUAGGGUUGCCGGUGAUCAGACGACUCGCCCGA | |
| Apta_271 | RNA | CUGUCGAGCAUGCUCAGGGUAACGUAUAGUCAAGACACCUCAAGUGUAACU | |
| Apta_520 | RNA | GGGAGAUCUACGGAUCUCAGGGCUCUUACGGGAGCUACAUGGAAGGAGUCCAUGUGU | |
| Apta_707 | RNA | GGCCUAAAACAUACCAGAUUUCGAUCUGGAGAGGUGAAGAAUUCGACCACCUAGGCCGGU | |
| Apta_1226 | RNA | GGAGGCGCCAACUGAAUGAAGGUGUAUUUCAGUUAAAGAACUUGCUGCUUCGGCAGCGUUCUGAGUUACUAGUCCAAUAAUCCGUAACUAGUCGCGUCAC |
DEV TOOL
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