AptaDB: Result

Interaction ID: 355

Aptamer sequence: GGTGGTGGTGGTTGTGGTGGTGGTGG

Target unique ID: P19338

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_350	AS1411	Protein	NUCL_HUMAN	N/A	For fluoresecent labeling, 400 nM aptamer were added to cell growth media for 1 hour at 37°C and 5% CO2. Dulbecco's phosphate-buffered saline was used to wash cells	22703281

Aptamer Sequence:	GGTGGTGGTGGTTGTGGTGGTGGTGG
The optimal secondary structure in dot-bracket notation:	..........................
The centroid secondary structure in dot-bracket notation:	..........................

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_350	Description	AS1411
Aptamer chemistry	DNA	Length	26 nt
GC content	65.4%	Molecular weight	8,512.21 Da
Molarity of 1 μg/μl solution	117.48 μM	Number of G-quadruplexes	2
G-Score	21	Function	Targeted therapy
Sequence	GGTGGTGGTGGTTGTGGTGGTGGTGG
Applications	Internalize into many different cancer cell-types would increase the utility of aptamer-mediated delivery of therapeutic agents

Type	Detail	Type	Detail
Uniprot ID	P19338	Protein name	Nucleolin (Protein C23)
Gene name(s)	NCL	Organism	Nucleolin (Protein C23)
Status	reviewed in uniprot database	Length	710
Mass	76,614	Activity regulation	N/A
Involvement in disease	N/A	Catalytic activity	N/A
PDB ID(s)	2FC8;2FC9;2KRR;
Function	Nucleolin is the major nucleolar protein of growing eukaryotic cells.

No relevant experimental diagram	Interaction ID	355
	Target type	Protein
	Target unique ID	P19338
	Activity	N/A
	Binding Conditions/Buffer	For fluoresecent labeling, 400 nM aptamer were added to cell growth media for 1 hour at 37°C and 5% CO2. Dulbecco's phosphate-buffered saline was used to wash cells
	Assay	N/A
	PubMed ID	22703281

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_757	DNA	GGTGGTGGTGGTTGTGGTGGTGG	88.46%
Apta_1247	DNA	GGTGGTTGCTGTTGTTTGTGGTTGGTGTGC	73.33%
Apta_10	DNA	GGGGGGGGTGGCTGGGCGGGGTGGG	73.08%
Apta_25	DNA	TGTGGGGGTGGTTGGGCGGGGCTGC	73.08%
Apta_1205	DNA	GGCAGGTGGTGTTGGTTGGTTGTGCGTGGAGTTGG	68.57%
Apta_124	DNA	GGTGGTGGGGGGGGTTGGTAGGGTGTCTTC	66.67%
Apta_1085	DNA	TGGGGTGGTGGTGTGGGTGGGGGTTTGTTC	66.67%
Apta_1240	DNA	GGAGTAGTGGGGATTGTGGTGTATGTTGTC	66.67%
Apta_1241	DNA	GGTGTGGAGTGGTGTGTGTTGTTGTTTGTC	66.67%
Apta_1210	DNA	GGGGAGGCAGTGTGTTGTGTCGTGTGTGTGCTTGG	65.71%
Apta_12	DNA	TGAGGGGGTGGGTGGGACGGGTTGG	65.38%
Apta_13	DNA	AGGGGGGGGGGAGGGGTCGGGGTGG	65.38%
Apta_389	DNA	GCCTGTGGTGTTGGGGCGGGTGCG	65.38%
Apta_1193	DNA	GGGTTGGAGGTGGAGGGGAGTTGAGG	65.38%
Apta_71	DNA	GGCGAGTAGGTTGGTGTGGTTGGGGCTCGCC	64.52%
Apta_679	DNA	CAGGTGGCAGATCGGGTGTGGGTGGCCTGG	63.33%
Apta_1094	DNA	GGAATCGTGGGTGGTAGGGTAGGGGATGCA	63.33%
Apta_1238	DNA	GGTTTTGTGTTTGTGTTTTGTGGTTGTTGT	63.33%
Apta_1239	DNA	GGTGGATGGGGATGCTTTGTTAGTTTGTGC	63.33%
Apta_1243	DNA	GGTGTTGTTGTTGGCTAGTTTGTTGTGGGC	63.33%

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