AptaDB: Result

Interaction ID: 337

Aptamer sequence: GGGTTATATTACTCGGCCGGTGTAA

Target unique ID: P15941

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_328	5TRG1	Protein	MUC1_HUMAN	25.1 nM	150 nM NaCl, 5nM MgCl2, pH 7.4	19103663

Aptamer Sequence:	GGGTTATATTACTCGGCCGGTGTAA
The optimal secondary structure in dot-bracket notation:	((((......))))...........
The centroid secondary structure in dot-bracket notation:	((((......))))...........

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_328	Description	5TRG1
Aptamer chemistry	DNA	Length	25 nt
GC content	48.0%	Molecular weight	7,967.93 Da
Molarity of 1 μg/μl solution	125.50 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Targeted therapy
Sequence	GGGTTATATTACTCGGCCGGTGTAA
Applications	Internalized by epithelial cancer cells and can be precisely activated by light to kill such cells

Type	Detail	Type	Detail
Uniprot ID	P15941	Protein name	Mucin-1 (MUC-1) (Breast carcinoma-associated antigen DF3) (Cancer antigen 15-3) (CA 15-3) (Carcinoma-associated mucin) (Episialin) (H23AG) (Krebs von den Lungen-6) (KL-6) (PEMT) (Peanut-reactive urinary mucin) (PUM) (Polymorphic epithelial mucin) (PEM) (Tumor-associated epithelial membrane antigen) (EMA) (Tumor-associated mucin) (CD antigen CD227) [Cleaved into: Mucin-1 subunit alpha (MUC1-NT) (MUC1-alpha); Mucin-1 subunit beta (MUC1-beta) (MUC1-CT)]
Gene name(s)	MUC1 PUM	Organism	Mucin-1 (MUC-1) (Breast carcinoma-associated antigen DF3) (Cancer antigen 15-3) (CA 15-3) (Carcinoma-associated mucin) (Episialin) (H23AG) (Krebs von den Lungen-6) (KL-6) (PEMT) (Peanut-reactive urinary mucin) (PUM) (Polymorphic epithelial mucin) (PEM) (Tumor-associated epithelial membrane antigen) (EMA) (Tumor-associated mucin) (CD antigen CD227) [Cleaved into: Mucin-1 subunit alpha (MUC1-NT) (MUC1-alpha); Mucin-1 subunit beta (MUC1-beta) (MUC1-CT)]
Status	reviewed in uniprot database	Length	1255
Mass	122,102	Activity regulation	N/A
Involvement in disease	MUC1/CA 15-3 is used as a serological clinical marker of breast cancer to monitor response to breast cancer treatment and disease recurrence	Catalytic activity	N/A
PDB ID(s)	1SM3;2ACM;2FO4;5T6P;5T78;6FZQ;6FZR;6KX1;6TGG;7Q4I;
Function	The alpha subunit has cell adhesive properties.

No relevant experimental diagram	Interaction ID	337
	Target type	Protein
	Target unique ID	P15941
	Activity	25.1 nM
	Binding Conditions/Buffer	150 nM NaCl, 5nM MgCl2, pH 7.4
	Assay	N/A
	PubMed ID	19103663

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_489	DNA	GGGGTTAAATATTCCCACATTGCCTGCGCCAGTACAAATAG	53.66%
Apta_809	DNA	GGGTACGTATTTGGTTTATAGATAGTAA	53.57%
Apta_1247	DNA	GGTGGTTGCTGTTGTTTGTGGTTGGTGTGC	53.33%
Apta_8	DNA	TGTGGGGGTGGACGGGCCGGGTAGA	52.00%
Apta_125	DNA	TGGTGGATGGCGCAGTCGGCGACAA	52.00%
Apta_147	DNA	TGCGGTTGTAGTACTCGTGGCCG	52.00%
Apta_538	DNA	GTGTTGTCTCTCTGTGTCTC	52.00%
Apta_1027	DNA	GGAGCTACGTTTAGCGAGTCCGACGCTCGGTGCCTCTTC	51.28%
Apta_779	DNA	TACGGGCTGGATCCACTGTTACGGCGTGTATCCGCTATCAA	51.22%
Apta_160	DNA	CGGTGCAGTGGATACATGCCAGCCGTAGCCATCGTGGATA	50.00%
Apta_260	DNA	GACGTTAAGCTAGCAGGTGTTAGGTCCCGTGGTGATGAAT	50.00%
Apta_477	DNA	CGCGGAAGAATTGCTATGTGTGCCCCGGTTGGTCTGTATA	50.00%
Apta_577	DNA	ACTTCAGTGAGTTGTCCCACGGTCGGCGAGTCGGTGGTAG	50.00%
Apta_1237	DNA	GGGGTTGTGTGGGGAGTTTGTCCTGTTGTG	50.00%
Apta_1243	DNA	GGTGTTGTTGTTGGCTAGTTTGTTGTGGGC	50.00%
Apta_1246	DNA	GGAGATGTATGAGCGGGTGTTTGTGTTTGC	50.00%
Apta_1156	DNA	TTCTGGTGTGGTAACTCATGTATCGGTCGTGGGGGTCGTGGCATT	48.89%
Apta_1026	DNA	GGGGGGTGTATCGTTGACGAGTTGCGCGTGCGTCTCGTG	48.72%
Apta_162	DNA	CCGTACATACTACGTACGTACGTGAACAGAGGCAGAG	48.65%
Apta_1204	DNA	GGCACGATGTGGCTACATCGATCGCGGTACTGGTG	48.57%

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