AptaDB: Result

Interaction ID: 477

Aptamer sequence: CCCCCACATTCAAATGGCCCCCCTACTCAATTACTGCACA

Target unique ID: Q61727

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_472	R5B1	Protein	I10R1_MOUSE	>200 nM	150 mmol/L NaCl, 20 mmol/L KCl, and 2 mmol/L HEPES heated to 82 °C and slowly cooling to room temperature	22434135

Aptamer Sequence:	CCCCCACATTCAAATGGCCCCCCTACTCAATTACTGCACA
The optimal secondary structure in dot-bracket notation:	...(((........))).......................
The centroid secondary structure in dot-bracket notation:	...(((........))).......................

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_472	Description	R5B1
Aptamer chemistry	DNA	Length	40 nt
GC content	52.5%	Molecular weight	12,009.75 Da
Molarity of 1 μg/μl solution	83.27 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Targeted therapy
Sequence	CCCCCACATTCAAATGGCCCCCCTACTCAATTACTGCACA
Applications	Bound to IL-10 receptor on the cell surface and blocked IL-10 function in vitro

Type	Detail	Type	Detail
Uniprot ID	Q61727	Protein name	Interleukin-10 receptor subunit alpha (IL-10 receptor subunit alpha) (IL-10R subunit alpha) (IL-10RA) (CDw210a) (Interleukin-10 receptor subunit 1) (IL-10R subunit 1) (IL-10R1) (CD antigen CD210)
Gene name(s)	Il10ra Il10r	Organism	Interleukin-10 receptor subunit alpha (IL-10 receptor subunit alpha) (IL-10R subunit alpha) (IL-10RA) (CDw210a) (Interleukin-10 receptor subunit 1) (IL-10R subunit 1) (IL-10R1) (CD antigen CD210)
Status	reviewed in uniprot database	Length	575
Mass	64,248	Activity regulation	N/A
Involvement in disease	N/A	Catalytic activity	N/A
PDB ID(s)	N/A
Function	Cell surface receptor for the cytokine IL10 that participates in IL10-mediated anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation.

No relevant experimental diagram	Interaction ID	477
	Target type	Protein
	Target unique ID	Q61727
	Activity	>200 nM
	Binding Conditions/Buffer	150 mmol/L NaCl, 20 mmol/L KCl, and 2 mmol/L HEPES heated to 82 °C and slowly cooling to room temperature
	Assay	N/A
	PubMed ID	22434135

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_473	DNA	CCCTGCACTCGCAATGGCCCCTCTACACCACAAACGGACA	65.00%
Apta_283	DNA	ACTCCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGA	55.00%
Apta_664	DNA	CACGCAACAACACAACATGCCCAGCGCCTGGAACATATCCTATGAGTTAGTCCGCCCACA	55.00%
Apta_969	DNA	TAACCACCCCACCTCGCTCCCGTGACACTAATGCTAATTCCAA	53.49%
Apta_240	DNA	TTTTTAATTAAAGCTCGCCATCAAATAGCTTT	52.50%
Apta_254	DNA	CCCGCTGTTCTCCCCCCCCCGCTGAGATCCCTGT	52.50%
Apta_436	DNA	CCCCCGCATCACGCCGTGGTGCGATTGACACAATTGCAAT	52.50%
Apta_31	DNA	ATACCAGTCTATTCAATTGGGCCCGTCCGTATGGTGGGTGTGCTGGCCAGATAGTATGTGCAATCA	51.52%
Apta_777	DNA	GAGCCCTATCTCACACCGCACCCGCAAACTATCATCCTACATG	51.16%
Apta_819	DNA	GCGCCCTCTCACGTGGCACTCAGAGTGCCGGAAGTTCTGCGTTAT	51.11%
Apta_689	DNA	CCACACAACAGCCTACGTGGATACACCACATACATCCCATAACCCCGTGTCATGTGTCG	50.85%
Apta_884	DNA	ATACCAGCTTATTCAATTTGCACCACTTATTGTTACTAATCTGAGATAGTAAGTGCAATCT	50.82%
Apta_49	DNA	CTTCCATGTTGAGGCGTTTATACCAGTTATCATCGGACTA	50.00%
Apta_229	DNA	GGGGCACGTTTATCCGTCCCTCCTAGTGGCGTGCCCC	50.00%
Apta_251	DNA	GCCCTACATTCTGTGCGACTCCCCCCCCCGC	50.00%
Apta_252	DNA	CCCTTACCTACTCTGCTCCCCCCCCCCCTCGGCTGC	50.00%
Apta_307	DNA	CATTCACCATGGCCCCTTCCTACGTATGTTCTGCGGGTG	50.00%
Apta_665	DNA	CGCCAATCTCAAAGCCCGCCACCTGCCCCTCACTGCCCACCTGTGGAATCCATGTCGCTC	50.00%
Apta_872	DNA	ACCGCCTTCCACCGTTCTCCACCACCCCTCAAACAACCCT	50.00%
Apta_1018	DNA	ATCCCTGCAGTTTGGTAGGGTGCGAGCAACATCAGGCACCTGCAGG	50.00%

ABOUT