Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 677
Aptamer sequence: GGUCUUACGUCGUUCGCGACUAUUGGGAGACC
Target unique ID: 791
Aptamer ID Aptamer descriptor Target chemistry Target name Affinity Binding Conditions/Buffer PubMed ID
Apta_634 IL 42-32b Molecule DL-Isoleucine 200 μM 10 mM HEPES (pH 7.0), 300 mM NaCl, and 7.5 mM MgCl2 (buffer B) and 0.1 mM each of ZnCl2, CaCl2, and MnCl2 and 0.01 mM CuCl 9630252
Structure information of aptamer
Aptamer Sequence: GGUCUUACGUCGUUCGCGACUAUUGGGAGACC
The optimal secondary structure in dot-bracket notation: ((((((..((((....))))......))))))
The centroid secondary structure in dot-bracket notation: ((((((.(((((....))))....).))))))
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
Type Detail Type Detail
Aptamer ID Apta_634 Description IL 42-32b
Aptamer chemistry RNA Length 32 nt
GC content 56.3% Molecular weight 10,233.07 Da
Molarity of 1 μg/μl solution 97.72 μM Number of G-quadruplexes No QGRS found
G-Score N/A Function Targeted binding
Sequence GGUCUUACGUCGUUCGCGACUAUUGGGAGACC
Applications Binding to isoleucine
Target information
Type Detail Type Detail
PubChem ID 791 Molecular name DL-Isoleucine
Molecular formula C6H13NO2 Molecular weight 131.17
IUPAC name 2-amino-3-methylpentanoic acid InChIKey AGPKZVBTJJNPAG-UHFFFAOYSA-N
LogP 0.444 Topological polar surface area 63.32
Hydrogen bond acceptor 2 Hydrogen bond donor 2

Synonym(s):
DL-ISOLEUCINE; 443-79-8; 2-Amino-3-methylpentanoic acid; DL-Allo-isoleucine; Isoleucine, DL-; H-DL-allo-Ile-OH; DL-Alloisoleucine; CHEBI:38264; 3107-04-8; NCGC00181164-01; DSSTox_CID_26882; DSSTox_RID_81986; DSSTox_GSID_46882; 2095501-92-9; Isoleucine, allo-; Isoleucine, threo-; Alloisoleucine, L-; L-Isoleucine, allo-; L-Isoleucine, threo-; CAS-443-79-8; H-DL-Ile-OH; NSC46708; MFCD00065934; (dl)-isoleucine; Isoleucine #; 2-amino-3-methyl-pentanoic acid; Norvaline, threo-; L(+)-Isoleucine; Cyclohexylphenylether; Norvaline, erythro-; (+/-)-Isoleucine; L-Norvaline, threo-; (+/-)-erythro-form; ISOLEUCINE,(L); POLY-L-ISOLEUCINE; (2S,3S)-2-azaniumyl-3-methyl-pentanoate; (+/-)-erythro-Isoleucine; SCHEMBL8868; Acetic acid, (R*,R*)-; WLN: QVYZY2&1 -L; CHEMBL297800; 2-amino-3-methyl pentanoic acid; FEMA 3295; DTXSID80901480; NSC9958; WLN: QVYZY2&1 -L,ALLO; DL-2-Amino-3-methylpentanoic acid; NSC-9958; Pentanoic acid, [S-(R*,R*)]-; Pentanoic acid, [S-(R*,S*)]-; Tox21_112765; BBL100034; FD3037; NSC-46708; NSC206282; STL185601; AKOS000120120; AKOS016051419; Tox21_112765_1; MCULE-9861955295; NSC-206282; VA50491; .alpha.-Amino-.beta.-methylvaleric acid; NCGC00274084-01; AC-24098; AS-49219; SY017751; SY022275; SY022276; SY022277; SY022340; AB0054248; DB-017950; DB-043068; DB-043069; DL-Isoleucine (mixture of 4 stereoisomers); A0212; FT-0600464; FT-0620923; FT-0624403; FT-0625476; FT-0626870; FT-0631944; FT-0633618; FT-0773006; (+/-)-erythro-2-Amino-3-methylpentanoic acid; 2487-EP2270018A1; 2487-EP2277867A2; 2487-EP2280003A2; 2487-EP2284157A1; 2487-EP2284178A2; 2487-EP2284179A2; 2487-EP2289892A1; 2487-EP2292088A1; 2487-EP2295055A2; 2487-EP2295410A1; 2487-EP2295550A2; 2487-EP2298780A1; 2487-EP2301939A1; 2487-EP2305657A2; 2487-EP2305689A1; 2487-EP2311453A1; 2487-EP2311850A1; 2487-EP2316470A2; 7304-EP2272972A1; 7304-EP2272973A1; 7304-EP2277872A1; 7304-EP2295550A2; 7304-EP2316470A2; I-7875; A826561; SR-01000944853; SR-01000944853-1; Q27117434; Q60662693; Z1270446358; B821F6FC-012D-4267-ABE2-AA1F36808C01; 34464-35-2; 7004-09-3

Activity data
Interaction ID 677
Target type Molecule
Target unique ID 791
Activity 200 μM
Binding Conditions/Buffer

10 mM HEPES (pH 7.0), 300 mM NaCl, and 7.5 mM MgCl2 (buffer B) and 0.1 mM each of ZnCl2, CaCl2, and MnCl2 and 0.01 mM CuCl
Assay

Isocratic affinity elution from isoleucine agarose
PubMed ID 9630252
Similar aptamers
Aptamer ID Aptamer chemistry Sequence Similarity
Apta_709 RNA AGUGAUACCAGCAUCGUCUUGAUGCCCUUGGCAGCACU 55.26%
Apta_703 RNA GGGCGGAUGAGACGCUUGGCGUGUGCUGUGGAGAGUCAUCCG 54.76%
Apta_402 RNA GCCUGUAAGGUGGUCGGUGUGGCGAGUGUGUUAGGAGAGAUUGC 54.55%
Apta_1141 RNA ACGAGUCUGGUCCUUCCUUUGACGACUAUUGUUUGGUAUCGAUCAACAACAA 53.85%
Apta_138 RNA GGAGGUGCUCCGAAAGGAACUCC 53.12%
Apta_231 RNA GGGAGGACGAUGCGGGUGUGGGGCG 53.12%
Apta_1292 RNA GGCACGUGUAUUACCCUAGUGGUCGACGUGCC 53.12%
Apta_758 RNA GGCUUUCGGGCUUUCGGCAACAUCAGCCCCUCAGCC 52.78%
Apta_230 RNA GGGAGGACGAUGCGGGCGUGAAUGGUGUUGUGAGG 51.43%
Apta_289 RNA GGGGGCUUAUCAUUCCAUUUAGUGUUAUGAUAACC 51.43%
Apta_51 RNA GCCUUAGUAACGUGCUUUGAUGUCGAUUCGACAGGAGGC 51.28%
Apta_859 RNA GGGAGUCUGAAGUCGGACUUGUUAUCAAUUCACUUCAGACU 51.22%
Apta_1288 RNA GGCGCACUGGCGCUGCGCCUUCGGGCGCCAAUCGUAGCGUGUCGGCGCC 51.02%
Apta_172 RNA GGAGACAUCCCUCUUGUUGGUGGUGCCGUGUGGAUGUCUC 50.00%
Apta_303 RNA CCUCCGGCUCAUACCUUUUCGAAGACAAGCUUCGACAGGAGG 50.00%
Apta_603 RNA CUUGUCUGAACAAUUUCGUCUGCCUUUGCGUUCCGACGGC 50.00%
Apta_633 RNA GGCAUCGGAAAGUGGGUUGAUGUAAGUAACAGGCGAUGCC 50.00%
Apta_647 RNA GGAUCCCGACUGGCGAGAGCCAGGUAACGAAUGGAUCC 50.00%
Apta_647 RNA GGAUCCCGACUGGCGAGAGCCAGGUAACGAAUGGAUCC 50.00%
Apta_381 RNA AUUCUGUUGGCGAACUGUACGCAAGUACUGGAUGACAGCCUAUCUAU 48.94%

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