Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 1034
Aptamer sequence: CACCTAATACGACTCACTATAGCGGATCCGAGGGGGTAGCGGGTGGGCCGGTGGATGCGGGGCGCCGGCGCCTGGCTCGAACAAGCTTGC
Target unique ID: P05413
Aptamer ID Aptamer descriptor Target chemistry Target name Affinity Binding Conditions/Buffer PubMed ID
Apta_951 N53 Protein FABPH_HUMAN 0.3337 μM 50 mM sodium phosphate buffer, pH 7.4, 50 mM NaCl, 5 mM KCl, 2.5 mM MgCl2 27545084
Structure information of aptamer
Aptamer Sequence: CACCTAATACGACTCACTATAGCGGATCCGAGGGGGTAGCGGGTGGGCCGGTGGATGCGGGGCGCCGGCGCCTGGCTCGAACAAGCTTGC
The optimal secondary structure in dot-bracket notation: (((((..(((..(((..............)))...)))..)))))(((..((.((.((.(((((....))))).))))..))..)))...
The centroid secondary structure in dot-bracket notation: ..............(((....((...(((....)))..))..)))........((.((.(((((....))))).))))............
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
Type Detail Type Detail
Aptamer ID Apta_951 Description N53
Aptamer chemistry DNA Length 90 nt
GC content 66.7% Molecular weight 27,959.80 Da
Molarity of 1 μg/μl solution 35.77 μM Number of G-quadruplexes 3
G-Score 57 Function Targeted binding
Sequence CACCTAATACGACTCACTATAGCGGATCCGAGGGGGTAGCGGGTGGGCCGGTGGATGCGGGGCGCCGGCGCCTGGCTCGAACAAGCTTGC
Applications Binding to target proteins
Target information
Type Detail Type Detail
Uniprot ID P05413 Protein name Fatty acid-binding protein, heart (Fatty acid-binding protein 3) (Heart-type fatty acid-binding protein) (H-FABP) (Mammary-derived growth inhibitor) (MDGI) (Muscle fatty acid-binding protein) (M-FABP)
Gene name(s) FABP3 FABP11 MDGI Organism Fatty acid-binding protein, heart (Fatty acid-binding protein 3) (Heart-type fatty acid-binding protein) (H-FABP) (Mammary-derived growth inhibitor) (MDGI) (Muscle fatty acid-binding protein) (M-FABP)
Status reviewed in uniprot database Length 133
Mass 14,858 Activity regulation N/A
Involvement in disease N/A Catalytic activity N/A
PDB ID(s)
1G5W;1HMR;1HMS;1HMT;2HMB;3RSW;3WBG;3WVM;3WXQ;4TJZ;4TKB;4TKH;4TKJ;4WBK;5B27;5B28;5B29;5CE4;5HZ9;6AQ1;7EGO;
Function FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters.
Activity data
Interaction ID 1034
Target type Protein
Target unique ID P05413
Activity 0.3337 μM
Binding Conditions/Buffer

50 mM sodium phosphate buffer, pH 7.4, 50 mM NaCl, 5 mM KCl, 2.5 mM MgCl2
Assay

EMSA and CD for specifificity study of aptamer
PubMed ID 27545084
Similar aptamers
Aptamer ID Aptamer chemistry Sequence Similarity
Apta_950 DNA CACCTAATACGACTCACTATAGCGGATCCGAAGGGGGCGCGAGGTGTAAGGGTGTGGGGTGGTGGGTGGGCCTGGCTCGAACAAGCTTGC
81.11%
Apta_953 DNA TCACCTCATACGACTCACTATAGCGGATCCGAGCCGGGGTGTTCTGTTGGCGGGGGCGGTGGGCGGGCTGGCTCGAACAAGCTTGC 78.89%
Apta_952 DNA TCACCTCATACGACTCACTATAGCGGACCCCACTCACTGCTCGGACTCTCCCCCTCCCCGCCTGGCTCGAACAAGCTTGC 68.89%
Apta_151 DNA GCAATGGTACGGTACTTCCGCGGTCCGGGGTGGGTGGGTGGTGGGGGGTGCGGGGGGGCGGCCGCAAAAGTGCACGCTACTTTGCTAA 56.67%
Apta_589 DNA GGGAATTCGAGCTCGGTACCATCTGTGTAAGGGGTAAGGGGTGGGGGTGGGTACGTCTAGCTGCAGGCATGCAAGCTTGG 56.67%
Apta_589 DNA GGGAATTCGAGCTCGGTACCATCTGTGTAAGGGGTAAGGGGTGGGGGTGGGTACGTCTAGCTGCAGGCATGCAAGCTTGG 56.67%
Apta_864 DNA CTCCTCTGACTGTAACCACGACTAGGACGCTTGGGAGGGGGGGTGGGGTGTCCGGTCGCGGCATAGGTAGTCCAGAAGCC 54.44%
Apta_1102 DNA TACGACTCACTATAGGGATCCGTGACTGTACGGGCTCAGTCGTTACTTGAGAGTTGAATTCCCTTTAGTGAGGGTT 54.44%
Apta_418 DNA TGGCAGGAAGACAAACACATCGTCGCGGCCTTAGGAGGGGCGGACGGGGGGGGGCGTGGTCTGTGGTGCTGT 53.33%
Apta_708 DNA CGTACGGAATTCGCTAGCGGGCGGGGGTGCTGGGGGAATGGAGTGCTGCGTGCTGCGGGGATCCGAGCTCCACGTG 53.33%
Apta_1011 DNA GCTGTGTGACTCCTGCAATGGTAGCGGGTAGGGGAGGGAGGGTGAATGGAGGATGTTCATGGCAGCTGTATCTTGTCTCC 53.33%
Apta_1099 DNA ATCCATTGCCACTGACTACCAGCCGGGTGGGTGGGGGGGTTTGCTGGTATCGCATTGTGCGAAGTCAGTCGGTCGTTAGT 53.33%
Apta_1101 DNA CCGTAATACGACTCACTATAGGTATGTGACGCGCGTCAATCGCTGTCCTACCAAGCTTTGCAGAGAGGATCCTT 53.33%
Apta_1105 DNA TACGACTCACTATAGGGATCCAATGGCACAGCGCCTGGAACGTACTCTGTACCTGGAATTCCCTTTAGTGAGGGTT 53.33%
Apta_281 DNA CCGTAATACGACTCACTATAGGGGAGCTCGGTACCGAATTCATGTTGGTAGTTGGCTTGTGTTCGTGTGTTAAGCTTTCAGAGAGGATCCTT 53.26%
Apta_587 DNA GGAATGGATCCACATCCATGGATGGGCAATGCGGGGTGGAGAATGGTTGCCGCACTTCGGCTTCACTGCAGACTTGACGAAGCTT 52.22%
Apta_771 DNA GAATTCCGTCGGACAGCGGTATGCATGGGAGGTTCTGGAGGGGGTGGGTTGGCGTGCGTTCCAGATGGACGAATATCGTCTCCC 52.22%
Apta_772 DNA GAATTCAGTCGGACAGCGCGGTGTTGGTGGTGGGGTGGTTTGGGAATCTGTTCGGCTGGAGATGGACGAATATCGTCTCCC 52.22%
Apta_832 DNA CCGGAGGTGGGTGAGGTCTGCGGCAGGCTGTGTGGGTGGACCGGAGGGGGGTGAGGTCTGCGGCAGGCTGTGTGGGTGGA 52.22%
Apta_865 DNA CTCCTCTGACTGTAACCACGTGTGCCAAAGAGAGTGGTGGGGGGGTGGGCGGAACTCGCGGCATAGGTAGTCCAGAAGCC 52.22%

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AptaDB is an online database we developed to integrate comprehensive information about aptamer.
The detail of this database is described here:

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