Aptamer-target interaction descriptor
Aptamer-target interaction information
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_253 | 2mHNE-5 | Protein | ELNE_HUMAN | 110 nM | 20 mM HEPES, pH 7.5, 150 mM NaCL, 6 mM KCL, 2 mM MgCl2 | 29568464 |
Structure information of aptamer
| Aptamer Sequence: | CCCTGTTCGCTATCCCCCATCCCCCGATTGC |
| The optimal secondary structure in dot-bracket notation: | ........((.(((...........))).)) |
| The centroid secondary structure in dot-bracket notation: | ........((..((...........))..)) |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_253 | Description | 2mHNE-5 |
| Aptamer chemistry | DNA | Length | 31 nt |
| GC content | 64.5% | Molecular weight | 9,494.88 Da |
| Molarity of 1 μg/μl solution | 105.32 μM | Number of G-quadruplexes | No QGRS found |
| G-Score | N/A | Function | Targeted binding |
| Sequence | CCCTGTTCGCTATCCCCCATCCCCCGATTGC | ||
| Applications | Binding to target proteins | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Uniprot ID | P08246 | Protein name | Neutrophil elastase (EC 3.4.21.37) (Bone marrow serine protease) (Elastase-2) (Human leukocyte elastase) (HLE) (Medullasin) (PMN elastase) |
| Gene name(s) | ELANE ELA2 | Organism | Neutrophil elastase (EC 3.4.21.37) (Bone marrow serine protease) (Elastase-2) (Human leukocyte elastase) (HLE) (Medullasin) (PMN elastase) |
| Status | reviewed in uniprot database | Length | 267 |
| Mass | 28,518 | Activity regulation | N/A |
| Involvement in disease | Cyclic haematopoiesis (CH) | Catalytic activity | Reaction=Hydrolysis of proteins, including elastin. Preferential cleavage: Val-|-Xaa > Ala-|-Xaa |
| PDB ID(s) | 1B0F;1H1B;1HNE;1PPF;1PPG;2RG3;2Z7F;3Q76;3Q77;4NZL;4WVP;5A09;5A0A;5A0B;5A0C;5A8X;5A8Y;5A8Z;5ABW;6E69;6F5M;6SMA;7CBK; |
 |
Function | Modifies the functions of natural killer cells, monocytes and granulocytes. |
Activity data
|
No relevant experimental diagram |
Interaction ID | 258 |
| Target type | Protein | |
| Target unique ID | P08246 | |
| Activity | 110 nM | |
| Binding Conditions/Buffer | 20 mM HEPES, pH 7.5, 150 mM NaCL, 6 mM KCL, 2 mM MgCl2 |
|
| Assay | N/A |
|
| PubMed ID | 29568464 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_252 | DNA | CCCTTACCTACTCTGCTCCCCCCCCCCCTCGGCTGC | |
| Apta_249 | DNA | CCCTGTTCCCCCTCCGCCGCTTGACTCTCC | |
| Apta_473 | DNA | CCCTGCACTCGCAATGGCCCCTCTACACCACAAACGGACA | |
| Apta_254 | DNA | CCCGCTGTTCTCCCCCCCCCGCTGAGATCCCTGT | |
| Apta_965 | DNA | CACGGCATGTGTGGCCGGATGCCTATATCGGTCGACTGCA | |
| Apta_1263 | DNA | CCCCGCTGCCCGTGTTCCGTCCTCCCTTGCTGTGTGTGCG | |
| Apta_250 | DNA | CCCTGGCCGCCCTCTGGCGCGCTGATGT | |
| Apta_1203 | DNA | CCCTGCGGGGCTACCCGATATGTGTCCGAGTGGTG | |
| Apta_1192 | DNA | CTTTCTGTCCTTCCGTCACATCCCACGCATTCTCCACAT | |
| Apta_7 | DNA | CCAGTCTCCCGTTTACCGCGCCTACACATGTCTGAATGCC | |
| Apta_221 | DNA | GTCCTAGCAGACACTCGGCCATCACGCCCATTGGTTTGCA | |
| Apta_817 | DNA | TGCGTGAGCGGTAGGCCCCTACGACCCACTGTGGTTGGGC | |
| Apta_251 | DNA | GCCCTACATTCTGTGCGACTCCCCCCCCCGC | |
| Apta_541 | DNA | CACGTGTTGTCTCTCTGTGTCTCGTG | |
| Apta_542 | DNA | GCACGTGTTGTCTCTCTGTGTCTCGTGC | |
| Apta_1083 | DNA | TGCTGTTGTCACCTGCCTCGTCTCCCTCGT | |
| Apta_1084 | DNA | TTCCCTCCTCTGTTGTTCCCCCCTCCTCTC | |
| Apta_1282 | DNA | TCCTCTCACACATCTTATCCCCTAGGG | |
| Apta_868 | DNA | CCCAGAAACAGCACCCTTACATCGACTGGACCTTCCGCG | |
| Apta_49 | DNA | CTTCCATGTTGAGGCGTTTATACCAGTTATCATCGGACTA |
DEV TOOL
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