AptaDB: Result

Interaction ID: 1203

Aptamer sequence: ACGAGUUGUCGCAUGUGCGGUUCAGUCUGGUCCUUCAGCACCGUACAACAA

Target unique ID: Q15063

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_1143	PNDA-3	Protein	POSTN_HUMAN	1.07 nM	40 mmol/l HEPES, pH 7.2, 102 mmol/l NaCl, 5 mmol/l mol/lgCl2, 5 mmol/l KCl	23511245

Aptamer Sequence:	ACGAGUUGUCGCAUGUGCGGUUCAGUCUGGUCCUUCAGCACCGUACAACAA
The optimal secondary structure in dot-bracket notation:	.(((....)))..((((((((.....((((....)))).))))))))....
The centroid secondary structure in dot-bracket notation:	.(((....)))..((((((((...(.((((....)))))))))))))....

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_1143	Description	PNDA-3
Aptamer chemistry	RNA	Length	51 nt
GC content	52.9%	Molecular weight	16,299.67 Da
Molarity of 1 μg/μl solution	61.35 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Targeted therapy
Sequence	ACGAGUUGUCGCAUGUGCGGUUCAGUCUGGUCCUUCAGCACCGUACAACAA
Applications	Inhibits breast cancer growth and metastasis

Type	Detail	Type	Detail
Uniprot ID	Q15063	Protein name	Periostin (PN) (Osteoblast-specific factor 2) (OSF-2)
Gene name(s)	POSTN OSF2	Organism	Periostin (PN) (Osteoblast-specific factor 2) (OSF-2)
Status	reviewed in uniprot database	Length	836
Mass	93,314	Activity regulation	N/A
Involvement in disease	N/A	Catalytic activity	N/A
PDB ID(s)	5WT7;5YJG;5YJH;
Function	Induces cell attachment and spreading and plays a role in cell adhesion.

	Interaction ID	1203
	Target type	Protein
	Target unique ID	Q15063
	Activity	1.07 nM
	Binding Conditions/Buffer	40 mmol/l HEPES, pH 7.2, 102 mmol/l NaCl, 5 mmol/l mol/lgCl2, 5 mmol/l KCl
	Assay	Binding affinities were determined using 32P-labeled PNDAs and varying hPN concentrations
	PubMed ID	23511245

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_1141	RNA	ACGAGUCUGGUCCUUCCUUUGACGACUAUUGUUUGGUAUCGAUCAACAACAA	57.69%
Apta_1144	RNA	ACGAGUCUGGUCCUUCCUCCCUAAUUGCUGUUGAGGUAUCGGCUACAACAA	56.86%
Apta_645	RNA	CUGGACGACGGGGACGCCACUGGACUAGGUAAGCCAGGACCGUACGUCGGGAGCCGUCAGAAUA	53.12%
Apta_132	RNA	GGGAGGACGAUGCGGCGUUUCCUCUGGUUCGUCCCCAGACGACUCGCCCGA	52.94%
Apta_1142	RNA	ACGAGUCACACGUUGAUGACUGGAUGGUAGUUAAAGAGGGUGGGGCAACAA	52.94%
Apta_1211	RNA	ACUAGCCUCAUCAGCUCAUGUGCCCCUCCGCCUGGAUCAC	52.94%
Apta_81	RNA	GCAAUGGUACGGUACUUCCAAGUGCGGGGGGGAGGUGGUGGUUCCAAAAGUGCACGCUACUUUGCUAA	51.47%
Apta_294	RNA	AUACCAGCUUAUUCAAUUGCCUGAUUAGCGGUAUCACGAUUACUUACCUUCGUUGCUGAGAUAGUAAGUGCAAUCU	51.32%
Apta_1	RNA	ACGAAGCUUGAUCCCGUUUGCCGGUCGAUCGCUUCGA	50.98%
Apta_92	RNA	ACAACUUAAUGCGACGUUGGUAAAGCAUAUCAAAC	50.98%
Apta_98	RNA	CGAUGCGGUCUCAUGCGUCGAGUGUGAGUUUACCUUCG	50.98%
Apta_135	RNA	GGGAGGACGAUGCGGAUUCUGGUUACUGGCCGCCCCAGACGACUCGCCCGA	50.98%
Apta_136	RNA	CGUGUCGUCCUGGUACGAUUUGGUAUAUAACCGUGGCUUUUCGACA	50.98%
Apta_367	RNA	CGAGCGUAGAAUGUCUUCCCACUAGACGUCGUUCGGCCCC	50.98%
Apta_603	RNA	CUUGUCUGAACAAUUUCGUCUGCCUUUGCGUUCCGACGGC	50.98%
Apta_606	RNA	CGGUUGGAUGGGUGUUGGUGGUUUGUUGAGUCCAGGCGGC	50.98%
Apta_632	RNA	GACGAGAAGGAGUGCUGGUUAUACUAGCGGUUAGGUCACUCGUC	50.98%
Apta_709	RNA	AGUGAUACCAGCAUCGUCUUGAUGCCCUUGGCAGCACU	50.98%
Apta_758	RNA	GGCUUUCGGGCUUUCGGCAACAUCAGCCCCUCAGCC	50.98%
Apta_574	RNA	AUGAAAAGGGCUGGCGAGACAUAUCCGCUGGGCAAUCAGAUUCGGAGCCGCACCACCCUCGAAGUAGACA	50.00%

ABOUT