AptaDB: Result

Interaction ID: 49

Aptamer sequence: CTTCCATGTTGAGGCGTTTATACCAGTTATCATCGGACTA

Target unique ID: P00432

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_49	CAT 3	Protein	CATA_BOVIN	43.4 μM	25 mM Tris, 192 mM glycine, 5 mM potassium phosphate buffer (pH 8.3)	22965726

Aptamer Sequence:	CTTCCATGTTGAGGCGTTTATACCAGTTATCATCGGACTA
The optimal secondary structure in dot-bracket notation:	..((((((.(((...((....))...))).))).)))...
The centroid secondary structure in dot-bracket notation:	..((((((.(((..............))).))).)))...

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_49	Description	CAT 3
Aptamer chemistry	DNA	Length	40 nt
GC content	42.5%	Molecular weight	12,251.89 Da
Molarity of 1 μg/μl solution	81.62 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Targeted binding
Sequence	CTTCCATGTTGAGGCGTTTATACCAGTTATCATCGGACTA
Applications	Aptamer exhibited a distinct specificity toward bovine catalase

Type	Detail	Type	Detail
Uniprot ID	P00432	Protein name	Catalase (EC 1.11.1.6)
Gene name(s)	CAT	Organism	Catalase (EC 1.11.1.6)
Status	reviewed in uniprot database	Length	527
Mass	59,915	Activity regulation	N/A
Involvement in disease	N/A	Catalytic activity	Reaction=2 H2O2 = 2 H2O + O2
PDB ID(s)	1TGU;1TH2;1TH3;1TH4;3J7B;3NWL;3RE8;3RGP;3RGS;4BLC;5GKN;6JNT;6JNU;6PM7;6PO0;7CAT;7DI8;8CAT;
Function	Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide.

	Interaction ID	49
	Target type	Protein
	Target unique ID	P00432
	Activity	43.4 μM
	Binding Conditions/Buffer	25 mM Tris, 192 mM glycine, 5 mM potassium phosphate buffer (pH 8.3)
	Assay	N/A
	PubMed ID	22965726

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_1065	DNA	CCTCGCTATGATGGAGTGCGTTTAGATCAGGGAACGGGTT	60.00%
Apta_1256	DNA	GATCGGGTGTGGGTGGCGTAAAGGGAGCATCGGACA	57.50%
Apta_302	DNA	ATCCTGTGAGGAATGCTCATGCATAGCAAGGGCT	55.00%
Apta_395	DNA	AATCCACGTATCGACTAGCTCAAACCAGCCTGATAGAGCCATTCGCTTTA	54.00%
Apta_641	DNA	TTCAAGACGATGCCTGGCGCGAGTTACACACTTGCATGGAGCTGG	53.33%
Apta_597	DNA	TACTGGGCTGGGCTTGGATTAGATCACAGTGGAACATACAGGACACACG	53.06%
Apta_184	DNA	AGGAAGGCTTTAGGTCTGAGATCTCGGAAT	52.50%
Apta_191	DNA	AGGAAGGCTTTACCTCTGAGATCTCGGAAT	52.50%
Apta_192	DNA	AGGAAGGCTTTAGCTCTGAGATCTCGGAAT	52.50%
Apta_215	DNA	AGGAAGGCTTTAGGTCCGAGATCTCGGAAT	52.50%
Apta_850	DNA	CACCAGTGTGTTGAGGTTTGAGCACACTGATAGAGTGTCA	52.50%
Apta_965	DNA	CACGGCATGTGTGGCCGGATGCCTATATCGGTCGACTGCA	52.50%
Apta_1176	DNA	AGTATGATATCGGTGTTTATGGTGTCTGTCTTCATACT	52.50%
Apta_1087	DNA	CTTACGACTCAGGCATTTTGCCGGGTAACGAAGTTACTGTCGTAAG	52.17%
Apta_1177	DNA	TCCTACTCGTGATATCGGTGTTTATGGTGTCTGTCTTCATGTAGGA	52.17%
Apta_344	DNA	CACTACAGAGGTTGCGTCTGTCCCACGTTGTCATGGGGGGTTGGCCTG	52.08%
Apta_644	DNA	TCAATCTAACCATGCATGCAGTTTAGGCAGGATTCGTTATCGCAA	51.11%
Apta_748	DNA	AGCTTGAGGGTGGGCGGGTGGACGCGGTAGTGGTATATAGGTCGG	51.11%
Apta_1126	DNA	TCGGGCGAGTCGTCTGCGGGCGGATAGACTACGACACCGCATCGTCCTCCC	50.98%
Apta_1280	DNA	TCATATCCTTCTTGGTATCATTGCGTCATTTAGTCATTCATGTTATTCCCGAGGG	50.91%

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