AptaDB: Result

Interaction ID: 235

Aptamer sequence: GGGAGGACGAUGCGGGCGUGAAUGGUGUUGUGAGG

Target unique ID: P06734

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_230	lGELl.2	Protein	FCER2_HUMAN	30 nM	PBS containing 1 mM MgCl2 (pH 7.4)	8683119

Aptamer Sequence:	GGGAGGACGAUGCGGGCGUGAAUGGUGUUGUGAGG
The optimal secondary structure in dot-bracket notation:	......(((((((...........)))))))....
The centroid secondary structure in dot-bracket notation:	......(((((((...........)))))))....

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_230	Description	lGELl.2
Aptamer chemistry	RNA	Length	35 nt
GC content	62.9%	Molecular weight	11,770.82 Da
Molarity of 1 μg/μl solution	84.96 μM	Number of G-quadruplexes	2
G-Score	17	Function	Targeted binding
Sequence	GGGAGGACGAUGCGGGCGUGAAUGGUGUUGUGAGG
Applications	IGEL1.2 and D17.4 competitively inhibit the interaction of human IgE with FceRI

Type	Detail	Type	Detail
Uniprot ID	P06734	Protein name	Low affinity immunoglobulin epsilon Fc receptor (BLAST-2) (C-type lectin domain family 4 member J) (Fc-epsilon-RII) (Immunoglobulin E-binding factor) (Lymphocyte IgE receptor) (CD antigen CD23) [Cleaved into: Low affinity immunoglobulin epsilon Fc receptor membrane-bound form; Low affinity immunoglobulin epsilon Fc receptor soluble form]
Gene name(s)	FCER2 CD23A CLEC4J FCE2 IGEBF	Organism	Low affinity immunoglobulin epsilon Fc receptor (BLAST-2) (C-type lectin domain family 4 member J) (Fc-epsilon-RII) (Immunoglobulin E-binding factor) (Lymphocyte IgE receptor) (CD antigen CD23) [Cleaved into: Low affinity immunoglobulin epsilon Fc receptor membrane-bound form; Low affinity immunoglobulin epsilon Fc receptor soluble form]
Status	reviewed in uniprot database	Length	321
Mass	36,469	Activity regulation	N/A
Involvement in disease	N/A	Catalytic activity	N/A
PDB ID(s)	1T8C;1T8D;2H2R;2H2T;4EZM;4G96;4G9A;4GI0;4GJ0;4GJX;4GK1;4GKO;4J6J;4J6K;4J6L;4J6M;4J6N;4J6P;4J6Q;4KI1;5LGK;6Y0L;6Y0M;
Function	Low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21.

	Interaction ID	235
	Target type	Protein
	Target unique ID	P06734
	Activity	30 nM
	Binding Conditions/Buffer	PBS containing 1 mM MgCl2 (pH 7.4)
	Assay	Representative binding curves of oligonucleotide ligands to IgE. The fraction of "P-labeled oligonucleotides bound to nitrocellulose filters is plotted as a function of total protein concentration, and the data points were fitted as described
	PubMed ID	8683119

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_231	RNA	GGGAGGACGAUGCGGGUGUGGGGCG	68.57%
Apta_370	RNA	GGGAGGACGCGUCGCCGUAAUGGAUGUUUUGCUCCCUG	63.16%
Apta_560	RNA	GGAAGAGAUGGCGACUAAAACGACUUGUCGC	57.14%
Apta_703	RNA	GGGCGGAUGAGACGCUUGGCGUGUGCUGUGGAGAGUCAUCCG	57.14%
Apta_131	RNA	GGGAGGACGAUGCGGUCCUGUCGUCUGUUCGUCCCCAGACGACUCGCCCGA	56.86%
Apta_134	RNA	GGGAGGACGAUGCGGGCCGUUGUUGUGUGCCGCCCCAGACGACUCGCCCGA	56.86%
Apta_660	RNA	UGGGAGGAGUGCUGCUCUCCAGGCACUCUUGUUGCUCGGA	55.00%
Apta_132	RNA	GGGAGGACGAUGCGGCGUUUCCUCUGGUUCGUCCCCAGACGACUCGCCCGA	54.90%
Apta_135	RNA	GGGAGGACGAUGCGGAUUCUGGUUACUGGCCGCCCCAGACGACUCGCCCGA	54.90%
Apta_312	RNA	GGGAAAAGAGACGGCCGGCGCCAUAGCCGAGAUCCGAGGUGUUGCCGAGAA	54.90%
Apta_315	RNA	GGGAGGACGAUGCGGUCGUAUUAUCCGCUGCACGCCAGACGACUCGCCCGA	54.90%
Apta_241	RNA	GCUUGACGGAGAUCAAGGGCGAGUCUCAUACCAAGUUGAUGGGG	54.55%
Apta_371	RNA	GGCAGACCAUACGGGAGAGAAACUUGCC	54.29%
Apta_683	DNA	GCAGTACGATCGGGGGTGGGTGGATGTAGG	54.29%
Apta_925	RNA	GCUCGGCCAGGUCGCGGGUGGAUGUGAGGG	54.29%
Apta_926	RNA	UCGGCAGCGGUUAGAGAGGUGGUGUGCGGG	54.29%
Apta_1194	DNA	GGGAGAGGTGGGAGCGGGGCCAGGG	54.29%
Apta_1288	RNA	GGCGCACUGGCGCUGCGCCUUCGGGCGCCAAUCGUAGCGUGUCGGCGCC	53.06%
Apta_133	RNA	GGGAGGACGAUGCGGUGAGUCGUUCCCUUCGUCCCCAGACGACUCGCCCGA	52.94%
Apta_129	RNA	GGGAGGACGAUGCGGACACCGUUAAUCUGAGGCCCUGUCCUAUUCCUUCACGCCUCAGA	52.54%

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