AptaDB: Result

Interaction ID: 1107

Aptamer sequence: TGGCAAGAGGGTGTGCTTAAGGTGGACACGGTGGCTTAG

Target unique ID: Other-49

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_1032	AptHis-C	Other	Hexahistidine	20.8 nM	1% BSA, 0.1% Tween-20, 0.2 mg/mL of tRNA, DPBS, pH 7.4	32739349

Aptamer Sequence:	TGGCAAGAGGGTGTGCTTAAGGTGGACACGGTGGCTTAG
The optimal secondary structure in dot-bracket notation:	.(((......((((.((......))))))....)))...
The centroid secondary structure in dot-bracket notation:	.(((......((((.((......))))))....)))...

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_1032	Description	AptHis-C
Aptamer chemistry	DNA	Length	39 nt
GC content	56.4%	Molecular weight	12,223.84 Da
Molarity of 1 μg/μl solution	81.81 μM	Number of G-quadruplexes	2
G-Score	19	Function	Targeted binding
Sequence	TGGCAAGAGGGTGTGCTTAAGGTGGACACGGTGGCTTAG
Applications	AptHis-C can only specifically recognize recombinant proteins with the hexahistidine-tag in simple or complex situations, and not to those with other tags

Type	Detail	Type	Detail
Target ID	Other-49	Name	Hexahistidine

	Interaction ID	1107
	Target type	Other
	Target unique ID	Other-49
	Activity	20.8 nM
	Binding Conditions/Buffer	1% BSA, 0.1% Tween-20, 0.2 mg/mL of tRNA, DPBS, pH 7.4
	Assay	AptHis-1, AptHis-2, AptHis-3 and AptHis-C exhibited equal binding affinity to the target peptide, but not to the blank beads
	PubMed ID	32739349

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_12	DNA	TGAGGGGGTGGGTGGGACGGGTTGG	58.97%
Apta_1205	DNA	GGCAGGTGGTGTTGGTTGGTTGTGCGTGGAGTTGG	58.97%
Apta_674	DNA	GCACGATGGGGAAAGGGTCCCCCTGGGTTG	56.41%
Apta_1204	DNA	GGCACGATGTGGCTACATCGATCGCGGTACTGGTG	56.41%
Apta_1204	DNA	GGCACGATGTGGCTACATCGATCGCGGTACTGGTG	56.41%
Apta_1210	DNA	GGGGAGGCAGTGTGTTGTGTCGTGTGTGTGCTTGG	56.41%
Apta_746	DNA	AGAGTGGGGGGGTGGGTGGATTTGACAGGTGGCATGCTGGAGAGT	55.56%
Apta_750	DNA	ATGCGAACAGGTGGGTGGGTTGGGTGGATTGTTCGGCTTCTTGAT	55.56%
Apta_438	DNA	AGCCATGACGATGTCGTTACGTAGATGCAGAGACTCCTAA	55.00%
Apta_438	DNA	AGCCATGACGATGTCGTTACGTAGATGCAGAGACTCCTAA	55.00%
Apta_443	DNA	GGCCATGACGATGTCGTTACGTAGATGCAGAGACTCCTAA	55.00%
Apta_477	DNA	CGCGGAAGAATTGCTATGTGTGCCCCGGTTGGTCTGTATA	55.00%
Apta_596	DNA	GGTGGCTGTTGGTCGAGGCTTATTAGTGTAGTCAATGGAGGCAGGCGACG	54.00%
Apta_9	DNA	TGAGGGGGCGGCGGGGCCGGGCAGG	53.85%
Apta_10	DNA	GGGGGGGGTGGCTGGGCGGGGTGGG	53.85%
Apta_18	DNA	TGGGGGGTTGGACGGGCGGGGCTGC	53.85%
Apta_25	DNA	TGTGGGGGTGGTTGGGCGGGGCTGC	53.85%
Apta_30	DNA	AGAGGGGGGGGTGGAGGGGGCTAGA	53.85%
Apta_50	DNA	AGTGGCGAGGGGGGTAGGTAAAGTATCCTTAGTGTGTTG	53.85%
Apta_124	DNA	GGTGGTGGGGGGGGTTGGTAGGGTGTCTTC	53.85%

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