Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 775
Aptamer sequence: ATCCGTCACACCTGCTCTATTCCTCTGTTGAAGAACCACTTCCGGAAATAAGAGTGGTGTTGGCTCCCGTAT
Target unique ID: 186907
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_1052 | AFAB3 | Molecule | Aflatoxin B1 | 96.6 nM | 20 mM Tris-HCL, 100 mM NaCl, 2 mM MgCl2, 5 mM KCl, 1 mM CaCl2 | 24984866 |
Structure information of aptamer
| Aptamer Sequence: | ATCCGTCACACCTGCTCTATTCCTCTGTTGAAGAACCACTTCCGGAAATAAGAGTGGTGTTGGCTCCCGTAT |
| The optimal secondary structure in dot-bracket notation: | ....((((((((.((((((((..((((..((((.....))))))))))).))))))))).))))........ |
| The centroid secondary structure in dot-bracket notation: | ....((((((((.((((((((..((((..((((.....))))))))))).)))))))))).)))........ |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_1052 | Description | AFAB3 |
| Aptamer chemistry | DNA | Length | 72 nt |
| GC content | 48.6% | Molecular weight | 22,059.13 Da |
| Molarity of 1 μg/μl solution | 45.33 μM | Number of G-quadruplexes | No QGRS found |
| G-Score | N/A | Function | Targeted binding |
| Sequence | ATCCGTCACACCTGCTCTATTCCTCTGTTGAAGAACCACTTCCGGAAATAAGAGTGGTGTTGGCTCCCGTAT | ||
| Applications | Recognition of aflflatoxin B1 | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| PubChem ID | 186907 | Molecular name | Aflatoxin B1 |
| Molecular formula | C17H12O6 | Molecular weight | 312.27 |
| IUPAC name | (3S,7R)-11-methoxy-6,8,19-trioxapentacyclo[10.7.0.02,9.03,7.013,17]nonadeca-1,4,9,11,13(17)-pentaene-16,18-dione | InChIKey | OQIQSTLJSLGHID-WNWIJWBNSA-N |
| LogP | 2.277 | Topological polar surface area | 74.97 |
| Hydrogen bond acceptor | 6 | Hydrogen bond donor | 0 |
Synonym(s): AFLATOXIN B1; 1162-65-8; AFB1; UNII-9N2N2Y55MH; NSC-529592; 1H,11H-Cyclopenta[c]furo[3',2':4,5]furo[2,3-h][1]benzopyran-1,11-dione,2,3,6a,9a-tetrahydro-4-methoxy-, (6aR,9aS)-; AFBI; 9N2N2Y55MH; CHEBI:2504; 2,3,6aalpha,9aalpha-Tetrahydro-4-methoxycyclopenta(c)furo(3',2':4,5)furo(2,3-h)(1)benzopyran-1,11-dione; MFCD00869647; (6aR,9aS)-4-methoxy-2,3,6a,9a-tetrahydrocyclopenta[c]furo[3',2':4,5]furo[2,3-h]chromene-1,11-dione; Aflatoxin B; (3S,7R)-11-methoxy-6,8,19-trioxapentacyclo[10.7.0.02,9.03,7.013,17]nonadeca-1,4,9,11,13(17)-pentaene-16,18-dione; NSC 529592; HSDB 3453; NSC529592; CCRIS 12; Aflatoxin B1 2 microg/mL in Acetonitrile; Cyclopenta(c)furo(3',2':4,5)furo(2,3-h)(1)benzopyran-1,11-dione, 2,3,6a,9a-tetrahydro-4-methoxy-; Cyclopenta(c)furo(3',2':4,5)furo(2,3-h)(1)benzopyran-1,11-dione, 2,3,6a,9a-tetrahydro-4-methoxy-, (6aR,9aS)-; EINECS 214-603-3; BRN 1269174; Aflatoxin B1 from Aspergillus flavus, from Aspergillus flavus; (+/-)-Aflatoxin B1; BIDD:ER0313; SCHEMBL126480; Aflatoxin B1 Standard Solution; CHEMBL1697694; DTXSID9020035; Aflatoxin B1, reference material; DTXSID00873175; BDBM120261; ZINC402671; AMY22311; EX-A5480; HY-N6615; AKOS030241596; VA10187; NCGC00247669-01; 2,3,6aalpha,9aalpha-Tetrahydro-4-methoxycyclopenta(c)furo(2',3':4,5)furo(2,3-h)chromene-1,11-dione; Cyclopenta(c)furo(3',2':4,5)furo(2,3-h)(1)benzopyran-1,11-dione, 2,3,6aalpha,9aalpha-tetrahydro-4-methoxy-; CS-0034371; 162A658; Q4689278; WLN: T F5 C6 B655 DOV GV OO QO RUT&&TTJ LO1; Aflatoxin B1 solution, 20 mug/mL in methanol, analytical standard; Aflatoxin B1 solution, 2 mug/mL in acetonitrile, analytical standard; Aflatoxin B1 solution, 3 mug/mL in benzene:acetonitrile (98:2), analytical standard; Aflatoxin B1 solution, 3.79 mug/g in acetonitrile, ERM(R) certified Reference Material; Aflatoxin B1 solution, certified reference material, 20 mug/mL in methanol, ampule of 1 mL; 10279-73-9; Aflatoxin B1 solution, certified reference material, 3 mug/mL in benzene:acetonitrile (98:2), ampule of 1 mL; Cyclopenta[c]furo[3',5]furo[2,3-h][1]benzopyran-1,11-dione, 2,3,6a,9a-tetrahydro-4-methoxy-; Cyclopenta[c]furo[3',5]furo[2,3-h][1]benzopyran-1,11-dione, 2,3,6a,9a-tetrahydro-4-methoxy-, (6aR-cis)-; Cyclopenta[c]furo[3',5]furo[2,3-h][1]benzopyran-1,11-dione, 2,3,6a.alpha.,9a.alpha.-tetrahydro-4-methoxy-
Activity data
|
No relevant experimental diagram |
Interaction ID | 775 |
| Target type | Molecule | |
| Target unique ID | 186907 | |
| Activity | 96.6 nM | |
| Binding Conditions/Buffer | 20 mM Tris-HCL, 100 mM NaCl, 2 mM MgCl2, 5 mM KCl, 1 mM CaCl2 |
|
| Assay | N/A |
|
| PubMed ID | 24984866 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_537 | DNA | ATCCGTCACACCTGCTCTCCTCGAACTAGTTAGACCCTCCTCCAAGTCAACTTGTGGTGTTGGCTCCCGTAT | |
| Apta_1051 | DNA | ATCCGTCACACCTGCTCTATACACATTGTGTTTACTCCCAGTTTTTTAGACTTATGGTGTTGGCTCCCGTAT | |
| Apta_530 | DNA | ATCCGTCACACCTGCTCTGTGTACGCCCGTATTTACGTTCCTAGCAATTGCTATGTGGTGTTGGCTCCCGTAT | |
| Apta_524 | DNA | ATCCGTCACACCTGCTCTGGCATTAGTATTCCATAGCCGGCCAAGTCTATGTAGTGGTGTTGGCTCCCGTAT | |
| Apta_525 | DNA | ATCCGTCACACCTGCTCTCAATCTGAAATATTGCAAGCAGTGCTCACAATTTGTTGGTGTTGGCTCCCGTAT | |
| Apta_532 | DNA | ATCCGTCACACCTGCTCTAACTTACACATAATTCTAGGTTACATCTTGCTCATATGGTGTTGGCTCCCGTAT | |
| Apta_534 | DNA | ATCCGTCACACCTGCTCTTGTAGGGTTCCCACCCAATTCAGTTCCGTTAAACCATGGTGTTGGCTCCCGTAT | |
| Apta_534 | DNA | ATCCGTCACACCTGCTCTTGTAGGGTTCCCACCCAATTCAGTTCCGTTAAACCATGGTGTTGGCTCCCGTAT | |
| Apta_906 | DNA | ATCCGTCACACCTGCTCTCCCCGGCGTCCGTTTATTAGCAGACTTTGGCGGAATTGGTGTTGGCTCCCGTAT | |
| Apta_529 | DNA | ATCCGTCACACCTGCTCTACACTCCGCACGATCTTTTTTAGAACGCGTACCCGTTGGTGTTGGCTCCCGTAT | |
| Apta_815 | DNA | CATCCGTCACACCTGCTCTGGCCACTAACATGGGGACCAGGTGGTGTTGGCTCCCGTATC | |
| Apta_1050 | DNA | ATCCGTCACACCTGCTCTCACGTCCGTCAACCAGATTGATACTTTATCTTGTTGTGGTGTTGGCTCCCGTAT | |
| Apta_535 | DNA | ATCCGTCACACCTGCTCTTGGGGTTATTACTCGTGAGATTGGGAATAGGTTACATGGTGTTGGCTCCCGTAT | |
| Apta_826 | DNA | ATCCGTCACACCTGCTCTGCACGGGCTCAGTTTGGCTTTGTATCCTAAGAGGGATGGTGTTGGCTCCCGTAT | |
| Apta_899 | DNA | ATCCGTCACACCTGCTCTCGAATAGAGTATTCGATACTGACTTTATTTACCGTCTGGTGTTGGCTCCCGTAT | |
| Apta_511 | DNA | ATCCGTCACACCTGCTCTCAGGGGATCACATTCTTGACGGTGTGATACAGTGCCTGGTGTTGGCTCCCGTAT | |
| Apta_523 | DNA | ATCCGTCACACCTGCTCTGACGCTGGGGTCGACCCGGAGAAATGCATTCCCCTGTGGTGTTGGCTCCCGTAT | |
| Apta_536 | DNA | ATCCGTCACACCTGCTCTCGAATAGAGTACTCGATACTGACTTTATTTACCGTCTGGTGTTGGCTCCCGTAT | |
| Apta_738 | DNA | ATCGTCTGCTCCGTCCAATACCTGCATATACACTTTGCATGTGGTTTGGTGTGAGGTCGTGC | |
| Apta_737 | DNA | ATCGTCTGCTCCGTCCAATAGTGCATTGAAACTTCTGCATCCTCGTTTGGTGTGAGGTCGTGC |
DEV TOOL
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