PEPDIA-PLP-003381

Table 1: AR-23 MIC 6.25 μM against K. pneumoniae ATCC 10031.

Table 1: AR-23 MBC 25 μM against K. pneumoniae ATCC 10031.

Table 1: AR-23 MIC 25 μM against E. coli ATCC 25992.

Table 1: AR-23 MBC N/D against E. coli ATCC 25992. N/D stands for not detected antibacterial activity in our experimental conditions.

Table 2: AR-23 MIC 50 μM against K. pneumoniae 1711.

Table 2: AR-23 MBC N/D against K. pneumoniae 1711.

Table 2: AR-23 MIC N/D against K. pneumoniae 311.

Table 2: AR-23 MBC N/D against K. pneumoniae 311.

Table 2: AR-23 MIC 50 μM against K. pneumoniae 1745.

Table 2: AR-23 MBC N/D against K. pneumoniae 1745.

Table 2: AR-23 MIC 25 μM against K. pneumoniae 1746.

Table 2: AR-23 MBC N/D against K. pneumoniae 1746.

Table 2: AR-23 MIC 25 μM against E. coli 2267.

Table 2: AR-23 MBC N/D against E. coli 2267.

Table 2: AR-23 MIC 50 μM against E. coli 3140.

Table 2: AR-23 MBC N/D against E. coli 3140.

Table 2: AR-23 MIC 25 μM against E. coli 716.

Table 2: AR-23 MBC 50 μM against E. coli 716.

Table 2: AR-23 MIC 25 μM against E. coli 1376.

Table 2: AR-23 MBC 50 μM against E. coli 1376.

Table 2: AR-23 MIC 25 μM against E. coli 1441.

Table 2: AR-23 MBC N/D against E. coli 1441.

AR-23 and Hylin-a1 reduce biofilm at 50 μM by 80–100% for both bacteria. (K. pneumoniae)

AR-23 and Hylin-a1 reduce biofilm at 50 μM by 80–100% for both bacteria. (E. coli)

AR23, which works worse than RV-23 when used alone, when combined with the antibiotic, gives similar if not better results than RV-23, indeed, with calculated MIC values at 3.125 μM (peptide) and 0.625 μg/mL (antibiotic) against K. pneumoniae.

AR23 ... with calculated MIC values at ... 6.25 μM (peptide) and 1.25 μg/mL (antibiotic) against E. coli.

RV-23 was found to be more active than AR-23, with an inhibitory concentration of 50% (IC50) at ... 12.5 μM in co-treatment.

AR-23 was characterized by an IC50 at 10 μM in the same treatment (virus pre-treatment).

On the contrary, no activity was observed after the viral adsorption step (post-treatment), indicating that the peptides did not interfere later in the infection.

AR-23 consistently affected cell viability at 50 μM, and the 50% cytotoxic concentration was at 25 μM.

AR-23 exhibits a CC50 of 50 μM.