ProForma 2.0
validated simple linear
MEAFIVSAFKHQAEGFKLHYK
Back to Primary literature
Primary-literature peptidemedium evidence PepDiana evidence grade for a literature-derived identity; Medium indicates retained review flags, while High has no unresolved review flag.
PEPDIA-PLP-001978
M104
Aliases: M104
Normalized sequence
MEAFIVSAFKHQAEGFKLHYK
21 amino acids
Release PepDiana-V1-20260620
85
Activity records
1
Source papers
82
Positive
3
Negative / threshold
Versioned scientific descriptors
Physicochemical properties and standard representations
Reported properties
No separate physicochemical property was reported in the primary-literature identity record. PepDiana release values are shown as computed properties.
Computed properties
physchem-20260620Molecular weight
2481.8671 Da
Molecular formula
C117H173N29O29S
Theoretical pI
8.2311
Net charge at pH 7
0.6763
GRAVY
-0.1381
Instability index
8.0476
Aromaticity
0.1905
Topological polar surface area
938.89 Ų
cLogP
-4.7309
Hydrogen-bond donors
32
Hydrogen-bond acceptors
33
Rotatable bonds
83
Method: PepDiana sequence/structure descriptor pipeline
Tool: Biopython+RDKit · Biopython 1.85; RDKit 2024.09.6
Calculated: 2026-06-20T11:01:38.358971+00:00
Assumptions: ph=7; termini=free N-terminus and free C-terminus for simple linear peptides; stereochemistry=standard L-amino acids; smiles_length_limit=200
Sequence representations
HELM 2.0
validated simple linear
PEPTIDE1{M.E.A.F.I.V.S.A.F.K.H.Q.A.E.G.F.K.L.H.Y.K}$$$$V2.0
Canonical SMILES
validated
CCC(C)C(NC(=O)C(Cc1ccccc1)NC(=O)C(C)NC(=O)C(CCC(=O)O)NC(=O)C(N)CCSC)C(=O)NC(C(=O)NC(CO)C(=O)NC(C)C(=O)NC(Cc1ccccc1)C(=O)NC(CCCCN)C(=O)NC(Cc1c[nH]cn1)C(=O)NC(CCC(N)=O)C(=O)NC(C)C(=O)NC(CCC(=O)O)C(=O)NCC(=O)NC(Cc1ccccc1)C(=O)NC(CCCCN)C(=O)NC(CC(C)C)C(=O)NC(Cc1c[nH]cn1)C(=O)NC(Cc1ccc(O)cc1)C(=O)NC(CCCCN)C(=O)O)C(C)C
Isomeric SMILES
validated
CC[C@H](C)[C@H](NC(=O)[C@H](Cc1ccccc1)NC(=O)[C@H](C)NC(=O)[C@H](CCC(=O)O)NC(=O)[C@@H](N)CCSC)C(=O)N[C@H](C(=O)N[C@@H](CO)C(=O)N[C@@H](C)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](Cc1c[nH]cn1)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CCC(=O)O)C(=O)NCC(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](Cc1c[nH]cn1)C(=O)N[C@@H](Cc1ccc(O)cc1)C(=O)N[C@@H](CCCCN)C(=O)O)C(C)C
Paper-level observations
Experimental evidence grouped by activity
Antimicrobial 82 records
PMC12936117
82 observationsAntimicrobialAnti-Gram-Positive observation
Record detail Table 1: Isolate Kp6, MIC of meropenem (µg/mL) in the presence of 0 µg/mL peptide = 4.
- Measure
- MIC
- Value
- 4 µg/mL
- Target
- Klebsiella pneumoniae
- Assay
- Broth microdilution (checkerboard assay)
AntimicrobialAnti-Gram-Positive observation
Record detail Table 1: Isolate Kp6, MIC of meropenem (µg/mL) in the presence of 7 µg/mL peptide = 2.
- Measure
- MIC
- Value
- 2 µg/mL
- Target
- Klebsiella pneumoniae
- Assay
- Broth microdilution (checkerboard assay)
AntimicrobialAnti-Gram-Positive observation
Record detail Table 1: Isolate Kp6, MIC of meropenem (µg/mL) in the presence of 15 µg/mL peptide = 2.
- Measure
- MIC
- Value
- 2 µg/mL
- Target
- Klebsiella pneumoniae
- Assay
- Broth microdilution (checkerboard assay)
AntimicrobialAnti-Gram-Positive observation
Record detail Table 1: Isolate Kp6, MIC of meropenem (µg/mL) in the presence of 31 µg/mL peptide = 2.
- Measure
- MIC
- Value
- 2 µg/mL
- Target
- Klebsiella pneumoniae
- Assay
- Broth microdilution (checkerboard assay)
AntimicrobialAnti-Gram-Positive observation
Record detail Table 1: Isolate Kp6, MIC of meropenem (µg/mL) in the presence of 62 µg/mL peptide = 2.
- Measure
- MIC
- Value
- 2 µg/mL
- Target
- Klebsiella pneumoniae
- Assay
- Broth microdilution (checkerboard assay)
AntimicrobialAnti-Gram-Positive observation
Record detail Table 1: Isolate Kp6, MIC of meropenem (µg/mL) in the presence of 125 µg/mL peptide = 2.
- Measure
- MIC
- Value
- 2 µg/mL
- Target
- Klebsiella pneumoniae
- Assay
- Broth microdilution (checkerboard assay)
AntimicrobialAnti-Gram-Positive observation
Record detail Table 1: Isolate Kp3, MIC of meropenem (µg/mL) in the presence of 0 µg/mL peptide = 8.
- Measure
- MIC
- Value
- 8 µg/mL
- Target
- Klebsiella pneumoniae
- Assay
- Broth microdilution (checkerboard assay)
AntimicrobialAnti-Gram-Positive observation
Record detail Table 1: Isolate Kp3, MIC of meropenem (µg/mL) in the presence of 7 µg/mL peptide = 8.
- Measure
- MIC
- Value
- 8 µg/mL
- Target
- Klebsiella pneumoniae
- Assay
- Broth microdilution (checkerboard assay)
AntimicrobialAnti-Gram-Positive observation
Record detail Table 1: Isolate Kp3, MIC of meropenem (µg/mL) in the presence of 15 µg/mL peptide = 8.
- Measure
- MIC
- Value
- 8 µg/mL
- Target
- Klebsiella pneumoniae
- Assay
- Broth microdilution (checkerboard assay)
AntimicrobialAnti-Gram-Positive observation
Record detail Table 1: Isolate Kp3, MIC of meropenem (µg/mL) in the presence of 31 µg/mL peptide = 8.
- Measure
- MIC
- Value
- 8 µg/mL
- Target
- Klebsiella pneumoniae
- Assay
- Broth microdilution (checkerboard assay)
AntimicrobialAnti-Gram-Positive observation
Record detail Table 1: Isolate Kp3, MIC of meropenem (µg/mL) in the presence of 62 µg/mL peptide = 8.
- Measure
- MIC
- Value
- 8 µg/mL
- Target
- Klebsiella pneumoniae
- Assay
- Broth microdilution (checkerboard assay)
AntimicrobialAnti-Gram-Positive observation
Record detail Table 1: Isolate Kp3, MIC of meropenem (µg/mL) in the presence of 125 µg/mL peptide = 4.
- Measure
- MIC
- Value
- 4 µg/mL
- Target
- Klebsiella pneumoniae
- Assay
- Broth microdilution (checkerboard assay)
AntimicrobialAnti-Gram-Positive observation
Record detail Table 1: Isolate Kp7, MIC of meropenem (µg/mL) in the presence of 0 µg/mL peptide = 8.
- Measure
- MIC
- Value
- 8 µg/mL
- Target
- Klebsiella pneumoniae
- Assay
- Broth microdilution (checkerboard assay)
AntimicrobialAnti-Gram-Positive observation
Record detail Table 1: Isolate Kp7, MIC of meropenem (µg/mL) in the presence of 7 µg/mL peptide = 2.
- Measure
- MIC
- Value
- 2 µg/mL
- Target
- Klebsiella pneumoniae
- Assay
- Broth microdilution (checkerboard assay)
AntimicrobialAnti-Gram-Positive observation
Record detail Table 1: Isolate Kp7, MIC of meropenem (µg/mL) in the presence of 15 µg/mL peptide = 2.
- Measure
- MIC
- Value
- 2 µg/mL
- Target
- Klebsiella pneumoniae
- Assay
- Broth microdilution (checkerboard assay)
AntimicrobialAnti-Gram-Positive observation
Record detail Table 1: Isolate Kp7, MIC of meropenem (µg/mL) in the presence of 31 µg/mL peptide = 2.
- Measure
- MIC
- Value
- 2 µg/mL
- Target
- Klebsiella pneumoniae
- Assay
- Broth microdilution (checkerboard assay)
AntimicrobialAnti-Gram-Positive observation
Record detail Table 1: Isolate Kp7, MIC of meropenem (µg/mL) in the presence of 62 µg/mL peptide = 2.
- Measure
- MIC
- Value
- 2 µg/mL
- Target
- Klebsiella pneumoniae
- Assay
- Broth microdilution (checkerboard assay)
AntimicrobialAnti-Gram-Positive observation
Record detail Table 1: Isolate Kp7, MIC of meropenem (µg/mL) in the presence of 125 µg/mL peptide = 2.
- Measure
- MIC
- Value
- 2 µg/mL
- Target
- Klebsiella pneumoniae
- Assay
- Broth microdilution (checkerboard assay)
AntimicrobialAnti-Gram-Positive observation
Record detail Table 1: Isolate Kp19, MIC of meropenem (µg/mL) in the presence of 0 µg/mL peptide = 8.
- Measure
- MIC
- Value
- 8 µg/mL
- Target
- Klebsiella pneumoniae
- Assay
- Broth microdilution (checkerboard assay)
AntimicrobialAnti-Gram-Positive observation
Record detail Table 1: Isolate Kp19, MIC of meropenem (µg/mL) in the presence of 7 µg/mL peptide = 4.
- Measure
- MIC
- Value
- 4 µg/mL
- Target
- Klebsiella pneumoniae
- Assay
- Broth microdilution (checkerboard assay)
AntimicrobialAnti-Gram-Positive observation
Record detail Table 1: Isolate Kp19, MIC of meropenem (µg/mL) in the presence of 15 µg/mL peptide = 4.
- Measure
- MIC
- Value
- 4 µg/mL
- Target
- Klebsiella pneumoniae
- Assay
- Broth microdilution (checkerboard assay)
AntimicrobialAnti-Gram-Positive observation
Record detail Table 1: Isolate Kp19, MIC of meropenem (µg/mL) in the presence of 31 µg/mL peptide = 4.
- Measure
- MIC
- Value
- 4 µg/mL
- Target
- Klebsiella pneumoniae
- Assay
- Broth microdilution (checkerboard assay)
AntimicrobialAnti-Gram-Positive observation
Record detail Table 1: Isolate Kp19, MIC of meropenem (µg/mL) in the presence of 62 µg/mL peptide = 4.
- Measure
- MIC
- Value
- 4 µg/mL
- Target
- Klebsiella pneumoniae
- Assay
- Broth microdilution (checkerboard assay)
AntimicrobialAnti-Gram-Positive observation
Record detail Table 1: Isolate Kp19, MIC of meropenem (µg/mL) in the presence of 125 µg/mL peptide = 4.
- Measure
- MIC
- Value
- 4 µg/mL
- Target
- Klebsiella pneumoniae
- Assay
- Broth microdilution (checkerboard assay)
AntimicrobialAnti-Gram-Positive observation
Record detail Table 1: Isolate Kp23, MIC of meropenem (µg/mL) in the presence of 0 µg/mL peptide = 8.
- Measure
- MIC
- Value
- 8 µg/mL
- Target
- Klebsiella pneumoniae
- Assay
- Broth microdilution (checkerboard assay)
AntimicrobialAnti-Gram-Positive observation
Record detail Table 1: Isolate Kp23, MIC of meropenem (µg/mL) in the presence of 7 µg/mL peptide = 8.
- Measure
- MIC
- Value
- 8 µg/mL
- Target
- Klebsiella pneumoniae
- Assay
- Broth microdilution (checkerboard assay)
AntimicrobialAnti-Gram-Positive observation
Record detail Table 1: Isolate Kp23, MIC of meropenem (µg/mL) in the presence of 15 µg/mL peptide = 8.
- Measure
- MIC
- Value
- 8 µg/mL
- Target
- Klebsiella pneumoniae
- Assay
- Broth microdilution (checkerboard assay)
AntimicrobialAnti-Gram-Positive observation
Record detail Table 1: Isolate Kp23, MIC of meropenem (µg/mL) in the presence of 31 µg/mL peptide = 8.
- Measure
- MIC
- Value
- 8 µg/mL
- Target
- Klebsiella pneumoniae
- Assay
- Broth microdilution (checkerboard assay)
AntimicrobialAnti-Gram-Positive observation
Record detail Table 1: Isolate Kp23, MIC of meropenem (µg/mL) in the presence of 62 µg/mL peptide = 4.
- Measure
- MIC
- Value
- 4 µg/mL
- Target
- Klebsiella pneumoniae
- Assay
- Broth microdilution (checkerboard assay)
AntimicrobialAnti-Gram-Positive observation
Record detail Table 1: Isolate Kp23, MIC of meropenem (µg/mL) in the presence of 125 µg/mL peptide = 2.
- Measure
- MIC
- Value
- 2 µg/mL
- Target
- Klebsiella pneumoniae
- Assay
- Broth microdilution (checkerboard assay)
AntimicrobialAnti-Gram-Positive observation
Record detail Table 1: Isolate Kp31, MIC of meropenem (µg/mL) in the presence of 0 µg/mL peptide = 8.
- Measure
- MIC
- Value
- 8 µg/mL
- Target
- Klebsiella pneumoniae
- Assay
- Broth microdilution (checkerboard assay)
AntimicrobialAnti-Gram-Positive observation
Record detail Table 1: Isolate Kp31, MIC of meropenem (µg/mL) in the presence of 7 µg/mL peptide = 4.
- Measure
- MIC
- Value
- 4 µg/mL
- Target
- Klebsiella pneumoniae
- Assay
- Broth microdilution (checkerboard assay)
AntimicrobialAnti-Gram-Positive observation
Record detail Table 1: Isolate Kp31, MIC of meropenem (µg/mL) in the presence of 15 µg/mL peptide = 2.
- Measure
- MIC
- Value
- 2 µg/mL
- Target
- Klebsiella pneumoniae
- Assay
- Broth microdilution (checkerboard assay)
AntimicrobialAnti-Gram-Positive observation
Record detail Table 1: Isolate Kp31, MIC of meropenem (µg/mL) in the presence of 31 µg/mL peptide = 2.
- Measure
- MIC
- Value
- 2 µg/mL
- Target
- Klebsiella pneumoniae
- Assay
- Broth microdilution (checkerboard assay)
AntimicrobialAnti-Gram-Positive observation
Record detail Table 1: Isolate Kp31, MIC of meropenem (µg/mL) in the presence of 62 µg/mL peptide = 2.
- Measure
- MIC
- Value
- 2 µg/mL
- Target
- Klebsiella pneumoniae
- Assay
- Broth microdilution (checkerboard assay)
AntimicrobialAnti-Gram-Positive observation
Record detail Table 1: Isolate Kp31, MIC of meropenem (µg/mL) in the presence of 125 µg/mL peptide = 2.
- Measure
- MIC
- Value
- 2 µg/mL
- Target
- Klebsiella pneumoniae
- Assay
- Broth microdilution (checkerboard assay)
AntimicrobialAnti-Gram-Positive observation
Record detail Table 1: Isolate Kp33, MIC of meropenem (µg/mL) in the presence of 0 µg/mL peptide = 8.
- Measure
- MIC
- Value
- 8 µg/mL
- Target
- Klebsiella pneumoniae
- Assay
- Broth microdilution (checkerboard assay)
AntimicrobialAnti-Gram-Positive observation
Record detail Table 1: Isolate Kp33, MIC of meropenem (µg/mL) in the presence of 7 µg/mL peptide = 4.
- Measure
- MIC
- Value
- 4 µg/mL
- Target
- Klebsiella pneumoniae
- Assay
- Broth microdilution (checkerboard assay)
AntimicrobialAnti-Gram-Positive observation
Record detail Table 1: Isolate Kp33, MIC of meropenem (µg/mL) in the presence of 15 µg/mL peptide = 4.
- Measure
- MIC
- Value
- 4 µg/mL
- Target
- Klebsiella pneumoniae
- Assay
- Broth microdilution (checkerboard assay)
AntimicrobialAnti-Gram-Positive observation
Record detail Table 1: Isolate Kp33, MIC of meropenem (µg/mL) in the presence of 31 µg/mL peptide = 2.
- Measure
- MIC
- Value
- 2 µg/mL
- Target
- Klebsiella pneumoniae
- Assay
- Broth microdilution (checkerboard assay)
AntimicrobialAnti-Gram-Positive observation
Record detail Table 1: Isolate Kp33, MIC of meropenem (µg/mL) in the presence of 62 µg/mL peptide = 2.
- Measure
- MIC
- Value
- 2 µg/mL
- Target
- Klebsiella pneumoniae
- Assay
- Broth microdilution (checkerboard assay)
AntimicrobialAnti-Gram-Positive observation
Record detail Table 1: Isolate Kp33, MIC of meropenem (µg/mL) in the presence of 125 µg/mL peptide = 2.
- Measure
- MIC
- Value
- 2 µg/mL
- Target
- Klebsiella pneumoniae
- Assay
- Broth microdilution (checkerboard assay)
AntimicrobialAnti-Gram-Positive observation
Record detail Table 1: Isolate Kp5, MIC of meropenem (µg/mL) in the presence of 0 µg/mL peptide = 16.
- Measure
- MIC
- Value
- 16 µg/mL
- Target
- Klebsiella pneumoniae
- Assay
- Broth microdilution (checkerboard assay)
AntimicrobialAnti-Gram-Positive observation
Record detail Table 1: Isolate Kp5, MIC of meropenem (µg/mL) in the presence of 7 µg/mL peptide = 16.
- Measure
- MIC
- Value
- 16 µg/mL
- Target
- Klebsiella pneumoniae
- Assay
- Broth microdilution (checkerboard assay)
AntimicrobialAnti-Gram-Positive observation
Record detail Table 1: Isolate Kp5, MIC of meropenem (µg/mL) in the presence of 15 µg/mL peptide = 16.
- Measure
- MIC
- Value
- 16 µg/mL
- Target
- Klebsiella pneumoniae
- Assay
- Broth microdilution (checkerboard assay)
AntimicrobialAnti-Gram-Positive observation
Record detail Table 1: Isolate Kp5, MIC of meropenem (µg/mL) in the presence of 31 µg/mL peptide = 16.
- Measure
- MIC
- Value
- 16 µg/mL
- Target
- Klebsiella pneumoniae
- Assay
- Broth microdilution (checkerboard assay)
AntimicrobialAnti-Gram-Positive observation
Record detail Table 1: Isolate Kp5, MIC of meropenem (µg/mL) in the presence of 62 µg/mL peptide = 16.
- Measure
- MIC
- Value
- 16 µg/mL
- Target
- Klebsiella pneumoniae
- Assay
- Broth microdilution (checkerboard assay)
AntimicrobialAnti-Gram-Positive observation
Record detail Table 1: Isolate Kp5, MIC of meropenem (µg/mL) in the presence of 125 µg/mL peptide = 8.
- Measure
- MIC
- Value
- 8 µg/mL
- Target
- Klebsiella pneumoniae
- Assay
- Broth microdilution (checkerboard assay)
AntimicrobialAnti-Gram-Positive observation
Record detail Table 1: Isolate Kp112, MIC of meropenem (µg/mL) in the presence of 0 µg/mL peptide = 16.
- Measure
- MIC
- Value
- 16 µg/mL
- Target
- Klebsiella pneumoniae
- Assay
- Broth microdilution (checkerboard assay)
AntimicrobialAnti-Gram-Positive observation
Record detail Table 1: Isolate Kp112, MIC of meropenem (µg/mL) in the presence of 7 µg/mL peptide = 2.
- Measure
- MIC
- Value
- 2 µg/mL
- Target
- Klebsiella pneumoniae
- Assay
- Broth microdilution (checkerboard assay)
AntimicrobialAnti-Gram-Positive observation
Record detail Table 1: Isolate Kp112, MIC of meropenem (µg/mL) in the presence of 15 µg/mL peptide = 2.
- Measure
- MIC
- Value
- 2 µg/mL
- Target
- Klebsiella pneumoniae
- Assay
- Broth microdilution (checkerboard assay)
AntimicrobialAnti-Gram-Positive observation
Record detail Table 1: Isolate Kp112, MIC of meropenem (µg/mL) in the presence of 31 µg/mL peptide = 2.
- Measure
- MIC
- Value
- 2 µg/mL
- Target
- Klebsiella pneumoniae
- Assay
- Broth microdilution (checkerboard assay)
AntimicrobialAnti-Gram-Positive observation
Record detail Table 1: Isolate Kp112, MIC of meropenem (µg/mL) in the presence of 62 µg/mL peptide = 2.
- Measure
- MIC
- Value
- 2 µg/mL
- Target
- Klebsiella pneumoniae
- Assay
- Broth microdilution (checkerboard assay)
AntimicrobialAnti-Gram-Positive observation
Record detail Table 1: Isolate Kp112, MIC of meropenem (µg/mL) in the presence of 125 µg/mL peptide = 2.
- Measure
- MIC
- Value
- 2 µg/mL
- Target
- Klebsiella pneumoniae
- Assay
- Broth microdilution (checkerboard assay)
AntimicrobialAnti-Gram-Positive observation
Record detail Table 1: Isolate Kp121, MIC of meropenem (µg/mL) in the presence of 0 µg/mL peptide = 128.
- Measure
- MIC
- Value
- 128 µg/mL
- Target
- Klebsiella pneumoniae
- Assay
- Broth microdilution (checkerboard assay)
AntimicrobialAnti-Gram-Positive observation
Record detail Table 1: Isolate Kp121, MIC of meropenem (µg/mL) in the presence of 7 µg/mL peptide = 16.
- Measure
- MIC
- Value
- 16 µg/mL
- Target
- Klebsiella pneumoniae
- Assay
- Broth microdilution (checkerboard assay)
AntimicrobialAnti-Gram-Positive observation
Record detail Table 1: Isolate Kp121, MIC of meropenem (µg/mL) in the presence of 15 µg/mL peptide = 16.
- Measure
- MIC
- Value
- 16 µg/mL
- Target
- Klebsiella pneumoniae
- Assay
- Broth microdilution (checkerboard assay)
AntimicrobialAnti-Gram-Positive observation
Record detail Table 1: Isolate Kp121, MIC of meropenem (µg/mL) in the presence of 31 µg/mL peptide = 8.
- Measure
- MIC
- Value
- 8 µg/mL
- Target
- Klebsiella pneumoniae
- Assay
- Broth microdilution (checkerboard assay)
AntimicrobialAnti-Gram-Positive observation
Record detail Table 1: Isolate Kp121, MIC of meropenem (µg/mL) in the presence of 62 µg/mL peptide = 8.
- Measure
- MIC
- Value
- 8 µg/mL
- Target
- Klebsiella pneumoniae
- Assay
- Broth microdilution (checkerboard assay)
AntimicrobialAnti-Gram-Positive observation
Record detail Table 1: Isolate Kp121, MIC of meropenem (µg/mL) in the presence of 125 µg/mL peptide = 8.
- Measure
- MIC
- Value
- 8 µg/mL
- Target
- Klebsiella pneumoniae
- Assay
- Broth microdilution (checkerboard assay)
AntimicrobialAnti-BiofilmPositive observation
Record detail Table 2: Isolate Kp5, Meropenem MBIC (µg/mL) = 256.
- Measure
- other
- Value
- 256 (MBIC)
- Target
- Klebsiella pneumoniae
- Assay
- Crystal violet staining (biofilm inhibition assay)
AntimicrobialAnti-BiofilmPositive observation
Record detail Table 2: Isolate Kp5, Meropenem+Peptide MBIC (µg/mL) = 128, [Peptide] (µg/mL) = 50, 100, and 200.
- Measure
- other
- Value
- 128 (MBIC)
- Target
- Klebsiella pneumoniae
- Assay
- Crystal violet staining (biofilm inhibition assay)
AntimicrobialAnti-BiofilmPositive observation
Record detail Table 2: Isolate Kp6, Meropenem MBIC (µg/mL) = 256.
- Measure
- other
- Value
- 256 (MBIC)
- Target
- Klebsiella pneumoniae
- Assay
- Crystal violet staining (biofilm inhibition assay)
AntimicrobialAnti-BiofilmPositive observation
Record detail Table 2: Isolate Kp6, Meropenem+Peptide MBIC (µg/mL) = 128, [Peptide] (µg/mL) = 200.
- Measure
- other
- Value
- 128 (MBIC)
- Target
- Klebsiella pneumoniae
- Assay
- Crystal violet staining (biofilm inhibition assay)
AntimicrobialAnti-BiofilmPositive observation
Record detail Table 2: Isolate Kp33, Meropenem MBIC (µg/mL) = 256.
- Measure
- other
- Value
- 256 (MBIC)
- Target
- Klebsiella pneumoniae
- Assay
- Crystal violet staining (biofilm inhibition assay)
AntimicrobialAnti-BiofilmPositive observation
Record detail Table 2: Isolate Kp33, Meropenem+Peptide MBIC (µg/mL) = 128, [Peptide] (µg/mL) = 100.
- Measure
- other
- Value
- 128 (MBIC)
- Target
- Klebsiella pneumoniae
- Assay
- Crystal violet staining (biofilm inhibition assay)
AntimicrobialAnti-BiofilmPositive observation
Record detail Table 2: Isolate Kp112, Meropenem MBIC (µg/mL) = 512.
- Measure
- other
- Value
- 512 (MBIC)
- Target
- Klebsiella pneumoniae
- Assay
- Crystal violet staining (biofilm inhibition assay)
AntimicrobialAnti-BiofilmPositive observation
Record detail Table 2: Isolate Kp112, Meropenem+Peptide MBIC (µg/mL) = 256, [Peptide] (µg/mL) = 200.
- Measure
- other
- Value
- 256 (MBIC)
- Target
- Klebsiella pneumoniae
- Assay
- Crystal violet staining (biofilm inhibition assay)
AntimicrobialAnti-BiofilmPositive observation
Record detail Table 2: Isolate Kp121, Meropenem MBIC (µg/mL) = 512.
- Measure
- other
- Value
- 512 (MBIC)
- Target
- Klebsiella pneumoniae
- Assay
- Crystal violet staining (biofilm inhibition assay)
AntimicrobialAnti-BiofilmPositive observation
Record detail Table 2: Isolate Kp121, Meropenem+Peptide MBIC (µg/mL) = 256, [Peptide] (µg/mL) = 200.
- Measure
- other
- Value
- 256 (MBIC)
- Target
- Klebsiella pneumoniae
- Assay
- Crystal violet staining (biofilm inhibition assay)
AntimicrobialAnti-BiofilmPositive observation
Record detail Table 3: Isolate Kp5, Meropenem MBEC (µg/mL) = 2048.
- Measure
- other
- Value
- 2048 (MBEC)
- Target
- Klebsiella pneumoniae
- Assay
- MTT assay (biofilm eradication assay)
AntimicrobialAnti-BiofilmPositive observation
Record detail Table 3: Isolate Kp5, Meropenem+Peptide MBEC (µg/mL) = 1024. Table footnote: The concentration of peptide was 200 (µg/mL).
- Measure
- other
- Value
- 1024 (MBEC)
- Target
- Klebsiella pneumoniae
- Assay
- MTT assay (biofilm eradication assay)
AntimicrobialAnti-BiofilmPositive observation
Record detail Table 3: Isolate Kp33, Meropenem MBEC (µg/mL) = 2048.
- Measure
- other
- Value
- 2048 (MBEC)
- Target
- Klebsiella pneumoniae
- Assay
- MTT assay (biofilm eradication assay)
AntimicrobialAnti-BiofilmPositive observation
Record detail Table 3: Isolate Kp33, Meropenem+Peptide MBEC (µg/mL) = 1024. Table footnote: The concentration of peptide was 200 (µg/mL).
- Measure
- other
- Value
- 1024 (MBEC)
- Target
- Klebsiella pneumoniae
- Assay
- MTT assay (biofilm eradication assay)
AntimicrobialAnti-BiofilmPositive observation
Record detail Table 3: Isolate Kp6, Meropenem MBEC (µg/mL) = 512.
- Measure
- other
- Value
- 512 (MBEC)
- Target
- Klebsiella pneumoniae
- Assay
- MTT assay (biofilm eradication assay)
AntimicrobialAnti-BiofilmPositive observation
Record detail Table 3: Isolate Kp6, Meropenem+Peptide MBEC (µg/mL) = 256. Table footnote: The concentration of peptide was 200 (µg/mL).
- Measure
- other
- Value
- 256 (MBEC)
- Target
- Klebsiella pneumoniae
- Assay
- MTT assay (biofilm eradication assay)
AntimicrobialAnti-BiofilmPositive observation
Record detail Table 3: Isolate Kp112, Meropenem MBEC (µg/mL) = 2048.
- Measure
- other
- Value
- 2048 (MBEC)
- Target
- Klebsiella pneumoniae
- Assay
- MTT assay (biofilm eradication assay)
AntimicrobialAnti-BiofilmPositive observation
Record detail Table 3: Isolate Kp112, Meropenem+Peptide MBEC (µg/mL) = 2048. Table footnote: The concentration of peptide was 200 (µg/mL).
- Measure
- other
- Value
- 2048 (MBEC)
- Target
- Klebsiella pneumoniae
- Assay
- MTT assay (biofilm eradication assay)
AntimicrobialAnti-BiofilmPositive observation
Record detail Table 3: Isolate Kp121, Meropenem MBEC (µg/mL) = 2048.
- Measure
- other
- Value
- 2048 (MBEC)
- Target
- Klebsiella pneumoniae
- Assay
- MTT assay (biofilm eradication assay)
AntimicrobialAnti-BiofilmPositive observation
Record detail Table 3: Isolate Kp121, Meropenem+Peptide MBEC (µg/mL) = 2048. Table footnote: The concentration of peptide was 200 (µg/mL).
- Measure
- other
- Value
- 2048 (MBEC)
- Target
- Klebsiella pneumoniae
- Assay
- MTT assay (biofilm eradication assay)
AntimicrobialAnti-Gram-Negative / threshold
Record detail Notably, the peptide did not enhance meropenem’s activity when tested against the ten carbapenemase-negative (presumably blaOXA-48 negative) isolates.
- Measure
- MIC
- Value
- no enhancement
- Target
- Klebsiella pneumoniae
- Assay
- Broth microdilution (checkerboard assay)
AntimicrobialAnti-Gram-Negative / threshold
Record detail The peptide exhibited no intrinsic antibacterial activity when used alone against the bacteria, either in planktonic or in the biofilm state of living.
- Measure
- MIC
- Value
- no intrinsic antibacterial activity
- Target
- Klebsiella pneumoniae
- Assay
- Broth microdilution (checkerboard assay)
Toxicity 3 records
PMC12936117
3 observationsToxicityCytotoxicPositive observation
Record detail Cells treated with 200 µg/mL of peptide M104 for 24 h maintained a mean cell viability of 99.6%, which was not statistically different from the untreated control (p-value > 0.05).
- Measure
- cell_viability
- Value
- 99.6%
- Target
- HDFa
- Assay
- MTT assay
ToxicityCytotoxicPositive observation
Record detail Following 48 h of incubation with 200 µg/mL peptide M104, the mean cell viability significantly reduced to 90.1% (p-value < 0.05) compared to the untreated control.
- Measure
- cell_viability
- Value
- 90.1%
- Target
- HDFa
- Assay
- MTT assay
ToxicityHemolyticNegative / threshold
Record detail In the hemolysis assay, peptide M104 showed no detectable hemolytic activity at the highest concentration tested (200 µg/mL).
- Measure
- hemolysis
- Value
- no detectable hemolytic activity
- Target
- Not reported by source
- Assay
- Hemolysis assay (hemoglobin release at 540 nm)
Citation and release provenance
Cite this identity as PepDiana record PEPDIA-PLP-001978, release PepDiana-V1-20260620. Experimental observations retain their paper, mention, and activity-record identifiers.
Record updated: 2026-06-22T07:14:26.229247+00:00