Frontiers in Aging Neuroscience · 2023
Sequence evidence
YVRHLKYVRHLK
P21(Ac-YVRHLKYVRHLK- NH2) was purchased from GL Biochem Ltd. (Shanghai).
Experimental observations
Figure 3D: the cell viability of P21 was significantly reduced at 25 uM (p < 0.05), and the cytotoxic peptide concentration produced at this time was higher than that of other peptides, and the cytotoxicity of P21 itself was lower.
Figure 4: P21 makes the fluorescence intensity of Aβ1-42 aggregation significantly lower than that of the Aβ group (p < 0.05), which can achieve 100% inhibition.
Figure 5: P21 polypeptide has a good inhibitory effect on the PC12 cytotoxicity caused by the aggregation of Aβ1-42 at a low concentration ratio (1:0.25 and 1:0.5). After the concentration ratio of P21 reached 1:1, with the increase of P21 concentration (1:2, 1:4), cell viability continued to increase, reaching the maximum at 1:4, which was close to the normal level.
Table 3: KD (M) for P21 is 5.385 × 10−5.
Local peptide labels such as P1, PEP1, or peptide 1 are grouped only within this paper and are not assumed to represent the same molecule across publications.
