AptaDB: Result

Interaction ID: 826

Aptamer sequence: GTGGCCAGTCACTCAATTGGGTGTAGGGGTGGGGATTGTGGGTTG

Target unique ID: CRM-CCL-185

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_744	S6	Cell	vaccinia virus–infected and–uninfected lung cancer A549 cells	28.2 nM	5 mmol MgCl2 and 4.5 g glucose into 1 L of Dulbecco's PBS without calcium and magnesium	19684903

Aptamer Sequence:	GTGGCCAGTCACTCAATTGGGTGTAGGGGTGGGGATTGTGGGTTG
The optimal secondary structure in dot-bracket notation:	...(((...(((((....)))))....)))...............
The centroid secondary structure in dot-bracket notation:	.........(((((....)))))......................

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_744	Description	S6
Aptamer chemistry	DNA	Length	45 nt
GC content	57.8%	Molecular weight	14,131.02 Da
Molarity of 1 μg/μl solution	70.77 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Target recognition
Sequence	GTGGCCAGTCACTCAATTGGGTGTAGGGGTGGGGATTGTGGGTTG
Applications	Recognition of subtype non-small cell lung cancer

Type	Detail	Type	Detail
ATCC ID	CRM-CCL-185	Cell name	vaccinia virus-infected and-uninfected lung cancer A549 cells
Category	Human cells	Organism	Homo sapiens, human
Product type	Certified reference material	Cell type	epithelial cell
Morphology	epithelial	Tissue	Lung
Disease	Carcinoma	Product format	Frozen
Antigen expression	N/A	Genes expressed	N/A
Cell description	N/A
Application(s)	Assay development

	Interaction ID	826
	Target type	Cell
	Target unique ID	CRM-CCL-185
	Activity	28.2 nM
	Binding Conditions/Buffer	5 mmol MgCl2 and 4.5 g glucose into 1 L of Dulbecco's PBS without calcium and magnesium
	Assay	The binding of selected aptamers to A549 cells pre-treated with trypsin
	PubMed ID	19684903

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_942	DNA	AGTGGGTAAGGTCTGGTGGATTGTGGACGGGGGGCGGGGCAGTGGCTTGA	58.00%
Apta_676	DNA	TCAGTCCCGATCAGGTGTGGGTGGCATTGG	57.78%
Apta_683	DNA	GCAGTACGATCGGGGGTGGGTGGATGTAGG	57.78%
Apta_1036	DNA	ATACCAGTCTATTCAATTGGGCCCGTCCGTATGGTGGG	57.78%
Apta_1255	DNA	GGCGCATGATCATTCGGTGGGTAAGGTGGTGGTAACGTTG	57.78%
Apta_650	DNA	GCACACCGATGGCGGTCCTGTTTAGGGGGTGTGCGTGGGGAGGTGCGGTTGC	57.69%
Apta_1267	DNA	GGCACGGCAAAGGGGTACAGCCTACCGAACCGTGGCTGTAAGGGTGGTTGTGGTGTG	56.14%
Apta_1041	DNA	TTGGCCCCGCGTGATCATTGAGGGGAGGAGCCGAGGCGGGTCCAAGATTG	56.00%
Apta_753	DNA	GGGCACAGACGGAAGATGAGAATTGTGGGGCTTAGTATAGTGAGGTGCGTGT	55.77%
Apta_266	DNA	TGGATGGTAGCTCGGTCGGGGTGGGTGGGTTGGCAAGTCT	55.56%
Apta_267	DNA	GAGTGCGTAATGGTACGATTTGGGAAGTGGCTTGGGGTGG	55.56%
Apta_590	DNA	ACCATCTGTGTAAGGGGTAAGGGGTGGGGGTGGGTACGTCT	55.56%
Apta_646	DNA	TGGAGCTTGGATTGATGTGGTGTGTGAGTGCGGTGCCC	55.56%
Apta_673	DNA	GCAGTCCTAGATCGGGTGTGGCTGGCTTGG	55.56%
Apta_782	DNA	AGGTGTGACTCGATCTGTGGGGGTTGGGGGGTGGTTTTTCGGAA	55.56%
Apta_869	DNA	ACTAGGACGCTTGGGAGGGGGGGTGGGGTGTCCGGTCGCG	55.56%
Apta_1085	DNA	TGGGGTGGTGGTGTGGGTGGGGGTTTGTTC	55.56%
Apta_31	DNA	ATACCAGTCTATTCAATTGGGCCCGTCCGTATGGTGGGTGTGCTGGCCAGATAGTATGTGCAATCA	54.55%
Apta_988	DNA	AAGGATGACTGACGACGATGGGAATGGAGAGGAAGGGGATATAGGTATGTTGGATGTAG	54.24%
Apta_618	DNA	GCGGCAAATCAGCATACGAGTGTACAGGGACGGGACGGGTGGGTAAAAGGTGTCGCCTCAG	54.10%

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