Aptamer-target interaction descriptor
Aptamer-target interaction information
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_8 | VEap121 | Protein | VEGFA_HUMAN | 1.1 nM | 10% (v/v) human serum in TBSTE (TBSE containing 0.05% (v/v) of Tween-20) | 20110884 |
Structure information of aptamer
| Aptamer Sequence: | TGTGGGGGTGGACGGGCCGGGTAGA |
| The optimal secondary structure in dot-bracket notation: | ......(((......)))....... |
| The centroid secondary structure in dot-bracket notation: | ......(((......)))....... |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_8 | Description | VEap121 |
| Aptamer chemistry | DNA | Length | 25 nt |
| GC content | 72.0% | Molecular weight | 8,243.94 Da |
| Molarity of 1 μg/μl solution | 121.30 μM | Number of G-quadruplexes | 2 |
| G-Score | 21 | Function | Aptasensor |
| Sequence | TGTGGGGGTGGACGGGCCGGGTAGA | ||
| Applications | VEGF-detection | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Uniprot ID | P15692 | Protein name | Vascular endothelial growth factor A (VEGF-A) (Vascular permeability factor) (VPF) |
| Gene name(s) | VEGFA VEGF | Organism | Vascular endothelial growth factor A (VEGF-A) (Vascular permeability factor) (VPF) |
| Status | reviewed in uniprot database | Length | 232 |
| Mass | 27,042 | Activity regulation | N/A |
| Involvement in disease | Microvascular complications of diabetes 1 (MVCD1) | Catalytic activity | N/A |
| PDB ID(s) | 1BJ1;1CZ8;1FLT;1KAT;1KMX;1MJV;1MKG;1MKK;1QTY;1TZH;1TZI;1VGH;1VPF;1VPP;2FJG;2FJH;2QR0;2VGH;2VPF;3BDY;3P9W;3QTK;3S1B;3S1K;3V2A;4DEQ;4GLN;4GLS;4KZN;4QAF;4WPB;4ZFF;5DN2;5FV1;5FV2;5HHC;5HHD;5O4E;5T89;6BFT;6D3O;6T9D;6V7K;6Z13;6Z3F;6ZBR;6ZCD;6ZFL;7KEZ;7KF0;7KF1;7LL8;7LL9; |
 |
Function | Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. |
Activity data
 |
Interaction ID | 8 |
| Target type | Protein | |
| Target unique ID | O14786 | |
| Activity | 4.7 nM | |
| Binding Conditions/Buffer | 10 mM Tris-HCl, 100 mM NaCl, and 5 mM KCl (pH 7.4) |
|
| Assay | Detection of VEGF by plate assay |
|
| PubMed ID | 23237717 | |
|
No relevant experimental diagram |
Interaction ID | 519 |
| Target type | Protein | |
| Target unique ID | P15692 | |
| Activity | 1.1 nM | |
| Binding Conditions/Buffer | 10% (v/v) human serum in TBSTE (TBSE containing 0.05% (v/v) of Tween-20) |
|
| Assay | Detection of VEGF by plate assay |
|
| PubMed ID | 20110884 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_23 | DNA | TGTGGGGGTGGACGGGCCGAGTACG | |
| Apta_29 | DNA | AGAGGGGGGGGACGGCCCGGGTAGA | |
| Apta_16 | DNA | AGGGGGGGGGGACGGGACGGGTATA | |
| Apta_21 | DNA | TGTGGGGGAGGACGGGCGGGGATGC | |
| Apta_22 | DNA | TGTGGGGGTGGACTGGGTGGGTACC | |
| Apta_24 | DNA | TGTGGGGATGGATGGGCCGGGCTGC | |
| Apta_28 | DNA | AGTGGGGGGGGTGGAGCCGGGTAGA | |
| Apta_9 | DNA | TGAGGGGGCGGCGGGGCCGGGCAGG | |
| Apta_12 | DNA | TGAGGGGGTGGGTGGGACGGGTTGG | |
| Apta_18 | DNA | TGGGGGGTTGGACGGGCGGGGCTGC | |
| Apta_25 | DNA | TGTGGGGGTGGTTGGGCGGGGCTGC | |
| Apta_10 | DNA | GGGGGGGGTGGCTGGGCGGGGTGGG | |
| Apta_26 | DNA | AGGGGGGGGGGTAAGGGTGGGTAGA | |
| Apta_27 | DNA | AGGGGGAGGGGTGGGGCCGGGCAGA | |
| Apta_11 | DNA | AGAGGGGGGGGTCGGGGTGGGTCGC | |
| Apta_13 | DNA | AGGGGGGGGGGAGGGGTCGGGGTGG | |
| Apta_14 | DNA | AGCGGGGGGGGCTGGGCTGGGTGGG | |
| Apta_15 | DNA | TGAGGGGGGGGGAGGGCGGGGCTGC | |
| Apta_320 | DNA | GCACTCTGTGGGGGTGGACGGGCCGGGT | |
| Apta_30 | DNA | AGAGGGGGGGGTGGAGGGGGCTAGA |
DEV TOOL
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