Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 1062
Aptamer sequence: ATGAGTAGTGCGGAGGGATGTGAATACTGACGCGGTCATAGTCGCTGTTGTACCATTGA
Target unique ID: P04640
Aptamer ID Aptamer descriptor Target chemistry Target name Affinity Binding Conditions/Buffer PubMed ID
Apta_979 OC10 Protein OSTCN_RAT 148.4 nM 2 mM MgCl2, 50 mM Tris (pH 7.5) and 150 mM NaCl in distilled water 33379043
Structure information of aptamer
Aptamer Sequence: ATGAGTAGTGCGGAGGGATGTGAATACTGACGCGGTCATAGTCGCTGTTGTACCATTGA
The optimal secondary structure in dot-bracket notation: ...(((.((((((((.(((((((...(((...)))))))).)).)).)))))).)))..
The centroid secondary structure in dot-bracket notation: ...(((.((((((((.(((((((...(((...)))))))).)).)).)))))).)))..
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
Type Detail Type Detail
Aptamer ID Apta_979 Description OC10
Aptamer chemistry DNA Length 59 nt
GC content 49.2% Molecular weight 18,376.78 Da
Molarity of 1 μg/μl solution 54.42 μM Number of G-quadruplexes No QGRS found
G-Score N/A Function Aptasensor
Sequence ATGAGTAGTGCGGAGGGATGTGAATACTGACGCGGTCATAGTCGCTGTTGTACCATTGA
Applications Binding to target proteins
Target information
Type Detail Type Detail
Uniprot ID P04640 Protein name Osteocalcin (Bone Gla protein) (BGP) (Gamma-carboxyglutamic acid-containing protein)
Gene name(s) Bglap Bglap2 Organism Osteocalcin (Bone Gla protein) (BGP) (Gamma-carboxyglutamic acid-containing protein)
Status reviewed in uniprot database Length 99
Mass 10,927 Activity regulation N/A
Involvement in disease N/A Catalytic activity N/A
PDB ID(s)
N/A
Function Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.
Activity data
Interaction ID 1062
Target type Protein
Target unique ID P04640
Activity 148.4 nM
Binding Conditions/Buffer

2 mM MgCl2, 50 mM Tris (pH 7.5) and 150 mM NaCl in distilled water
Assay

Determination of affinity/dissociation constant (Kd) of from the saturation binding curve
PubMed ID 33379043
Similar aptamers
Aptamer ID Aptamer chemistry Sequence Similarity
Apta_746 DNA AGAGTGGGGGGGTGGGTGGATTTGACAGGTGGCATGCTGGAGAGT 57.63%
Apta_110 DNA AGCAGCACAGAGGTCAGATGGTTAGGAAGGTGAATAGGTACGACTGCAGGTCTATCGCAGCCTATGCGTGCTACCGTGAA 56.25%
Apta_110 DNA AGCAGCACAGAGGTCAGATGGTTAGGAAGGTGAATAGGTACGACTGCAGGTCTATCGCAGCCTATGCGTGCTACCGTGAA 56.25%
Apta_78 DNA ATCTCGACTAGTCATAGGGGGCGCGAACATACGCGGTTGGTGTGGTTGGCTGACAATACTCGT 55.56%
Apta_1035 DNA ATGCGGATCCCGCGCGGGTCTGAGGAGTGCGCGGTGCCAGTGAGTGCGCGAAGCTTGCGC 55.00%
Apta_79 DNA ATCTCGAGTCATAGGGGGCGCGAACATACGCGGTTGGTGTGGTTGGCTGACTCGT 54.24%
Apta_1013 DNA ATCACGTATGGGGGGCCGGCATGAGGGCCGCGCGTGACA 54.24%
Apta_1063 DNA AACTTATCGGAGCGTGATTTCCGTCTCGCCGCTTTCCTTT 54.24%
Apta_175 DNA AGCAGCACAGAGGTCAGATGAGGACTGTCGATAAGTATAATGGCAAGTAGCTCGATACATCCTATGCGTGCTACCGTGAA 53.75%
Apta_178 DNA AGCAGCACAGAGGTCAGATGCGGTATGCTGGGTCATCTAACTGGGGATATTCTGATATTGCCTATGCGTGCTACCGTGAA 53.75%
Apta_983 DNA ATGGGAACAGAGCGGCGCAGAGACCCAATGCCACGAGTACCTGACCCTATCAACCCGTGA 53.33%
Apta_1000 DNA ATAGGAGTCGACCGACCAGAACCGTTCCCATACTTCTCCAGCGATACTTAGTGCGTCTACATCTAGACTC 52.86%
Apta_511 DNA ATCCGTCACACCTGCTCTCAGGGGATCACATTCTTGACGGTGTGATACAGTGCCTGGTGTTGGCTCCCGTAT 52.78%
Apta_1109 DNA GGTATGTGTCGTTGATCTGGATATCTATGCCTAATGCTCGTAGGCGACTGTTCGGATAGTAACGTTGACCGCATAC 52.63%
Apta_143 DNA GGTGCGGTTCGTGCGGTTGTAGTACTCGTGGCCGATAGAGGTAGTTTCG 52.54%
Apta_263 DNA AGAGGGGAGGGTCGGGTATCGGCGTGTTCGGGGGATCTGC 52.54%
Apta_487 DNA CGGGGAGTTGGGAGGGGGGGCAAAGCCTGTGTATTTATCTTCTGT 52.54%
Apta_488 DNA CGACCAGGGTGGGAGGGGGACGGGCTGGCGGCGCGCGGTAATG 52.54%
Apta_569 DNA TGCGCATGTGAAAGGTTGCCTAACTGGACAGGGTTTAGGAGCGACTAGACACAG 52.54%
Apta_697 DNA CTCTCGGGACGACGGACGCTAATCTTACAAGGGCGTAGTGTATGTCGTCCC 52.54%

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AptaDB is an online database we developed to integrate comprehensive information about aptamer.
The detail of this database is described here:

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