Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 422
Aptamer sequence: GGACCUCGGCGAAAGCCGGUAACGCCACAAGUCGGAGGGUAAGAUCUGACAGGAACUGGGUGACGGAGGUUAGGUGC
Target unique ID: P69718
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_416 | L1-Rev | Protein | REV_HV1H3 | 18 μM | 20 mM Tris-HCl, pH 7.5, 50 mM NaCl, and 60 mM MgCl2 | 12531194 |
Structure information of aptamer
| Aptamer Sequence: | GGACCUCGGCGAAAGCCGGUAACGCCACAAGUCGGAGGGUAAGAUCUGACAGGAACUGGGUGACGGAGGUUAGGUGC |
| The optimal secondary structure in dot-bracket notation: | .(((((((((....))).....((((.((.((((((........))))))......))))))...))))))...... |
| The centroid secondary structure in dot-bracket notation: | .(((((((((....))).............((((((........))))))...............))))))...... |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_416 | Description | L1-Rev |
| Aptamer chemistry | RNA | Length | 77 nt |
| GC content | 59.7% | Molecular weight | 25,129.05 Da |
| Molarity of 1 μg/μl solution | 39.79 μM | Number of G-quadruplexes | 2 |
| G-Score | 19 | Function | Aptasensor |
| Sequence | GGACCUCGGCGAAAGCCGGUAACGCCACAAGUCGGAGGGUAAGAUCUGACAGGAACUGGGUGACGGAGGUUAGGUGC | ||
| Applications | Detection of diverse analytes with an aptazyme ligase array | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Uniprot ID | P69718 | Protein name | Protein Rev (ART/TRS) (Anti-repression transactivator) (Regulator of expression of viral proteins) |
| Gene name(s) | rev | Organism | Protein Rev (ART/TRS) (Anti-repression transactivator) (Regulator of expression of viral proteins) |
| Status | reviewed in uniprot database | Length | 116 |
| Mass | 13,051 | Activity regulation | N/A |
| Involvement in disease | N/A | Catalytic activity | N/A |
| PDB ID(s) | 3LPH;4PMI; |
 |
Function | Escorts unspliced or incompletely spliced viral pre-mRNAs (late transcripts) out of the nucleus of infected cells. |
Activity data
 |
Interaction ID | 422 |
| Target type | Protein | |
| Target unique ID | P69718 | |
| Activity | 18 μM | |
| Binding Conditions/Buffer | 20 mM Tris-HCl, pH 7.5, 50 mM NaCl, and 60 mM MgCl2 |
|
| Assay | Response curves for allosteric ribozyme ligases |
|
| PubMed ID | 12531194 | |
 |
Interaction ID | 1002 |
| Target type | Other | |
| Target unique ID | Other-29 | |
| Activity | 18 μM | |
| Binding Conditions/Buffer | 50 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2), r. t. |
|
| Assay | Response curves for allosteric ribozyme ligases |
|
| PubMed ID | 12531194 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_59 | RNA | GGACCUCGGCGAAAGCUAACGUCUCAUGGCUAAAUUGCCAUGUUGCUACAAAUGAUAUGACUAGAGAGGUUAGGUGCCUCGUGAUGUCCAGUCGC | |
| Apta_155 | RNA | GACCCAGGGCCGGGACGCAAAGCAGCCAAAACAGACGGCCCCAGUCAGGGAGUU | |
| Apta_561 | RNA | GGAUAAUAGCCGUAGGUUGCGAAAGCGACCCUGAUGAGCCUGUGGAAACAGACGUGGCACAUGACUACGUCGAAACGGUAGCGAGAGCUC | |
| Apta_563 | RNA | GGAUAAUAGCCGUAGGUUGCGAAAGCGACCCUGAUGAGAAAGCCAAAGCCGUAGCGCAGAUGAUCUCGCCAUCAGUACCGAAACGGUAGCGAGAGCUC | |
| Apta_613 | RNA | GGAUAAUAGCCGUAGGUUGCGAAAGCGACCCUGAUGAGCCUUAGGAUAUGCAUGAUGCAGAAGGACGUCGAAACGGUAGCGAGAGCUC | |
| Apta_562 | RNA | GGAUAAUAGCCGUAGGUUGCGAAAGCGACCCUGAUGAGCCUUUAGGGCCAAGUGUGGUGAAAGACACACUCGAAACGGUAGCGAGAGCUC | |
| Apta_566 | RNA | GGAUAAUAGCCGUAGGUUGCGAAAGCGACCCUGAUGAGCCCUGCGAUGCAGAAAGGUGCUGACGACACAUCGAAACGGUAGCGAGAGCUC | |
| Apta_380 | RNA | GGGAUGCUUCGGCAUCCCCGAAGCCGCUAUGGACCAGUGGCGCGGCUUCGGCCCGACGGAGUGGUACCGCUUCGGCGGUACGUAAGCUUGGG | |
| Apta_432 | RNA | GGGAGACAAGAAUAAACGCUCAAGGCAUUUCUGCUAGUCUGGUUGUAAGAUAUUCAACACGUGAGUUUCGACAGGAGGCUCACAACAGGC | |
| Apta_433 | RNA | GGGAGACAAGAAUAAACGCUCAACGUUCAGUAUAACAGUCCGAGUCUAACACACAAUGGGACACUGAAUUCGACAGGAGGCUCACAACAGGC | |
| Apta_710 | RNA | GGAUAAUAGCCGUAGGUUGCGAAAGCGACCCUGAUGAGUCUGGAUACCAUGCAUGAUGCACCUUGGCAGUCUUACGAAACGGUAGCGAGAGCUC | |
| Apta_594 | RNA | GGGCUAUUGCUGGAGGGGCGCCACAUGAAAGUGGUGGUUGGGUGCGGUCGGCGAUAGCUC | |
| Apta_645 | RNA | CUGGACGACGGGGACGCCACUGGACUAGGUAAGCCAGGACCGUACGUCGGGAGCCGUCAGAAUA | |
| Apta_353 | RNA | GAAUUCCGCGUGUGCCAGCGAAAGUUGCGUAUGGGUCACAUCGCAGGCACAUGUCAUCUGGGCGGUCCGUUCGGGAUCC | |
| Apta_168 | RNA | AAUUAACCCUCACUAAAGGGAACUGUUGUGAGUCUCAUGUCGAAGAGCGGUUAAGGGAGAUUUAGGCAGCAGCUUGGACAGUGUAUCGGCUGAGUUGAGCGUCUAGUCUUGUCU | |
| Apta_549 | RNA | GGAGCUCAGCCUUCACUGCAUGAUAAACCGAUGCUGGGCGAUUCUCCUGAAGUAGGGGAAGAGUUGUCAUGUAUGGGGGCACCACGGUCGGAUCCUG | |
| Apta_1221 | RNA | GGAGGCGCCAACUGAAUGAACGAGUUGAAAUGCGGAUCAAGUUACUGCUUCGGCAGUACCUAGUGGGAAAAGUUCUUGGGUCCGUAACUAGUCGCGUCAC | |
| Apta_1223 | RNA | GGAGGCGCCAACUGAAUGAAAGGUUACGCUUCAACGCCGUGCAGCUGCUUCGGCAGGCUUGUGAGUACAUGGCCCAUUGAUCCGUAACUAGUCGCGUCAC | |
| Apta_394 | RNA | GGGCGAAUUCAACUGCCAUCUAGGCAGUAACCAGGAGUUAGUAGGACAAGUUUCGUCC | |
| Apta_575 | RNA | AGAGCUUGACGGUCCCGAGAGUCGAGCCCAAGCUGACACUGGACCUUUGCGGACCACGUGUUGAUCGUCG |
DEV TOOL
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