Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 961
Aptamer sequence: GCCGCGACCATACAACGCAAACAACACGCCTGTACGCTTG
Target unique ID: Other-12
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_875 | WKB-3 | Other | P48 protein of Mycoplasma bovis (M. bovis) | 32.81 nM | P48 protein dissolved in 100 μL of 0.05 carbonate-bicarbonate buffer | 24417693 |
Structure information of aptamer
| Aptamer Sequence: | GCCGCGACCATACAACGCAAACAACACGCCTGTACGCTTG |
| The optimal secondary structure in dot-bracket notation: | ...(((....((((..((.........)).)))))))... |
| The centroid secondary structure in dot-bracket notation: | ...(((....((((..((.........)).)))))))... |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_875 | Description | WKB-3 |
| Aptamer chemistry | DNA | Length | 40 nt |
| GC content | 57.5% | Molecular weight | 12,188.86 Da |
| Molarity of 1 μg/μl solution | 82.04 μM | Number of G-quadruplexes | No QGRS found |
| G-Score | N/A | Function | Target detection |
| Sequence | GCCGCGACCATACAACGCAAACAACACGCCTGTACGCTTG | ||
| Applications | Detection of antibodies to mycoplasma bovis in serum | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Target ID | Other-12 | Name | P48 protein of Mycoplasma bovis (M. bovis) |
Activity data
 |
Interaction ID | 961 |
| Target type | Other | |
| Target unique ID | Other-12 | |
| Activity | 32.81 nM | |
| Binding Conditions/Buffer | P48 protein dissolved in 100 μL of 0.05 carbonate-bicarbonate buffer |
|
| Assay | Dose-dependent i-ELAA to determine the dissociation constant (Kd) of aptamers |
|
| PubMed ID | 24417693 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_309 | DNA | TAGCCAAGGTAACCAGTACAAGGTGCTAAACGTAATGGCTTCGGCTTAC | |
| Apta_309 | DNA | TAGCCAAGGTAACCAGTACAAGGTGCTAAACGTAATGGCTTCGGCTTAC | |
| Apta_221 | DNA | GTCCTAGCAGACACTCGGCCATCACGCCCATTGGTTTGCA | |
| Apta_1206 | DNA | GGCAGCAGCAATGTAACACTGTGTGTATGTGTTGG | |
| Apta_1232 | DNA | GGCCACACAAACAACATGACAACACGTCTCACATAACGCCCACGTGCTGCCGCCTCATCG | |
| Apta_641 | DNA | TTCAAGACGATGCCTGGCGCGAGTTACACACTTGCATGGAGCTGG | |
| Apta_663 | DNA | CACGCACAGAAGACACCTACAGGGCCAGATCACAATCGGTTAGTGAACTCGTACGGCGCG | |
| Apta_1228 | DNA | GGCCACCAAACCACACCGTCGCAACCGCGAGAACCGAAGTAGTGATCATGTCCCTGCGTG | |
| Apta_1264 | DNA | CAACGATCGTACCACAGTACCCACCCACCAGCCCCAACATCATGCCCATGCGTCGGTGTG | |
| Apta_154 | RNA | ACACGCGAGAUAGAGCGAGGCCUCCAAAAAUGGCCACGCCAGGAAGCAAGU | |
| Apta_1219 | DNA | GCGCAGAACCGACAGCAAAACGGAAGTCCACCACGACTATTGGTGCCCAATCTACCGTA | |
| Apta_227 | DNA | ACGCGCCTATGGTGCATCGCATGCCGACC | |
| Apta_324 | DNA | CAAACAATCAAACAGCAGTGGGGTG | |
| Apta_324 | DNA | CAAACAATCAAACAGCAGTGGGGTG | |
| Apta_1204 | DNA | GGCACGATGTGGCTACATCGATCGCGGTACTGGTG | |
| Apta_1204 | DNA | GGCACGATGTGGCTACATCGATCGCGGTACTGGTG | |
| Apta_937 | DNA | TTGCCCACAGATCTGTGGAAGCCGAACCGCGTGCTAGTCGTG | |
| Apta_939 | DNA | TTGCCCACCGTACTGTGCAGGTCGAACTACAGGCACGTCGTG | |
| Apta_1070 | DNA | CCGTATCGCCCAGGCAACTGGGCTAAACTTCCCAGAGGGAACGAAACCTGGG | |
| Apta_355 | DNA | GATGCAGCCACCTACATTGCAGGTTTTATAATACAACTACGCGCCGTTCGCAGGCTAGTT |
DEV TOOL
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