AptaDB: Result

Interaction ID: 48

Aptamer sequence: ACTGGGCAGATTAACGGTCCTATATTTTCGGTTCTGGAAA

Target unique ID: P00432

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_48	CAT 2	Protein	CATA_BOVIN	1.29 μM	25 mM Tris, 192 mM glycine, 5 mM potassium phosphate buffer (pH 8.3)	22965726

Aptamer Sequence:	ACTGGGCAGATTAACGGTCCTATATTTTCGGTTCTGGAAA
The optimal secondary structure in dot-bracket notation:	...((((.........))))....((((((....))))))
The centroid secondary structure in dot-bracket notation:	...((((.........)))).....((((......)))).

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_48	Description	CAT 2
Aptamer chemistry	DNA	Length	40 nt
GC content	42.5%	Molecular weight	12,340.95 Da
Molarity of 1 μg/μl solution	81.03 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Targeted binding
Sequence	ACTGGGCAGATTAACGGTCCTATATTTTCGGTTCTGGAAA
Applications	Aptamer exhibited a distinct specificity toward bovine catalase

Type	Detail	Type	Detail
Uniprot ID	P00432	Protein name	Catalase (EC 1.11.1.6)
Gene name(s)	CAT	Organism	Catalase (EC 1.11.1.6)
Status	reviewed in uniprot database	Length	527
Mass	59,915	Activity regulation	N/A
Involvement in disease	N/A	Catalytic activity	Reaction=2 H2O2 = 2 H2O + O2
PDB ID(s)	1TGU;1TH2;1TH3;1TH4;3J7B;3NWL;3RE8;3RGP;3RGS;4BLC;5GKN;6JNT;6JNU;6PM7;6PO0;7CAT;7DI8;8CAT;
Function	Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide.

	Interaction ID	48
	Target type	Protein
	Target unique ID	P00432
	Activity	1.29 μM
	Binding Conditions/Buffer	25 mM Tris, 192 mM glycine, 5 mM potassium phosphate buffer (pH 8.3)
	Assay	N/A
	PubMed ID	22965726

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_564	DNA	GGGGACTGTTGTGGTATTATTTTTGGTTGTGCAGT	57.50%
Apta_818	DNA	GGGCTGGCCAGATCAGACCCCGGATGATCATCCTTGTGAGAACCA	55.56%
Apta_215	DNA	AGGAAGGCTTTAGGTCCGAGATCTCGGAAT	55.00%
Apta_1236	DNA	GGACTGTTGTGGTATTATTTTTGGTTGTGC	55.00%
Apta_982	DNA	ATGACGGGGGTCTAGGCAAGTAATAACGGGGGCAAGCTTTTCTATCTCGTTCTAGGGTA	54.24%
Apta_750	DNA	ATGCGAACAGGTGGGTGGGTTGGGTGGATTGTTCGGCTTCTTGAT	53.33%
Apta_184	DNA	AGGAAGGCTTTAGGTCTGAGATCTCGGAAT	52.50%
Apta_186	DNA	AGGAACGCTTTAGGTCTGAGATCTCGGAAT	52.50%
Apta_193	DNA	AGGAAGGCTTTAGGGTCTGAGATCTCGGAAT	52.50%
Apta_194	DNA	AGGAAGGCTTTAGGGTCTGAGATCTCGGGAAT	52.50%
Apta_202	DNA	AAGGCTTTAGGTCTGAGATCTCGGAAT	52.50%
Apta_915	DNA	ACTTCGGCTAGTTAGGGGGTAGTTTAGATCGTCTCTACAT	52.50%
Apta_1177	DNA	TCCTACTCGTGATATCGGTGTTTATGGTGTCTGTCTTCATGTAGGA	52.17%
Apta_776	DNA	AGTGGTCGAACTACACATCCTTGAACTGCGGAATTATCTAC	51.22%
Apta_846	DNA	GTGCAGACAGCTAGTAGTGGTGACTTTCTGTCCAAATCAAGATAA	51.11%
Apta_1278	DNA	CCATGATTCAACTTTACTGGTCTTGTCTTGGCTAGTCGTGTGTCATTCCCTAAGG	50.91%
Apta_1277	DNA	TCCGTGGTACGGTTACTCGTTCCTTATGTATGTTGCTACAATGGATTGCGTCCCCAGGG	50.85%
Apta_36	DNA	GTTAATGGGGGATCTCGCGGCCGTTCTTGTTGCTTATACA	50.00%
Apta_159	DNA	GCGTGCAGCATGACCACCGATGTCACACTTAATGAGAAT	50.00%
Apta_165	DNA	TGGACGAACTGCCCTCAGCTACTTTCATGTTGCTGACGCA	50.00%

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