AptaDB: Result

Interaction ID: 834

Aptamer sequence: GCTATCTTATGGAAATTTCGTGTAGGGTTTGGTGTGGCGGGGCTA

Target unique ID: CRM-CCL-185

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_752	S15	Cell	vaccinia virus–infected and–uninfected lung cancer A549 cells	56.8 nM	5 mmol MgCl2 and 4.5 g glucose into 1 L of Dulbecco's PBS without calcium and magnesium	19684903

Aptamer Sequence:	GCTATCTTATGGAAATTTCGTGTAGGGTTTGGTGTGGCGGGGCTA
The optimal secondary structure in dot-bracket notation:	.....((((((((...)))))).))(((((.((...)).))))).
The centroid secondary structure in dot-bracket notation:	.....((((.............))))...................

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_752	Description	S15
Aptamer chemistry	DNA	Length	45 nt
GC content	48.9%	Molecular weight	14,039.98 Da
Molarity of 1 μg/μl solution	71.23 μM	Number of G-quadruplexes	2
G-Score	21	Function	Target recognition
Sequence	GCTATCTTATGGAAATTTCGTGTAGGGTTTGGTGTGGCGGGGCTA
Applications	Recognition of subtype non-small cell lung cancer

Type	Detail	Type	Detail
ATCC ID	CRM-CCL-185	Cell name	vaccinia virus-infected and-uninfected lung cancer A549 cells
Category	Human cells	Organism	Homo sapiens, human
Product type	Certified reference material	Cell type	epithelial cell
Morphology	epithelial	Tissue	Lung
Disease	Carcinoma	Product format	Frozen
Antigen expression	N/A	Genes expressed	N/A
Cell description	N/A
Application(s)	Assay development

	Interaction ID	834
	Target type	Cell
	Target unique ID	CRM-CCL-185
	Activity	56.8 nM
	Binding Conditions/Buffer	5 mmol MgCl2 and 4.5 g glucose into 1 L of Dulbecco's PBS without calcium and magnesium
	Assay	The binding of selected aptamers to A549 cells pre-treated with trypsin
	PubMed ID	19684903

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_493	DNA	GGGGTGGTGGGTTCTTTTTGTGGTATTGGTGTGGGGGGCA	62.22%
Apta_364	DNA	TATGGTATGCTGTGTGGTATGGGGTGGCGTGCTCT	57.78%
Apta_1091	DNA	CCATGGTAGGTATTGCTTGGTAGGGATAGTGGGCTTGATG	57.78%
Apta_1092	DNA	CCCATGGTAGGTATTGCTTGGTAGGGATAGTGGGCTTGATG	57.78%
Apta_47	DNA	GACCTAGCAGTGGACATGTGGCAGGGTGAAGTGGCATCGTC	55.56%
Apta_646	DNA	TGGAGCTTGGATTGATGTGGTGTGTGAGTGCGGTGCCC	55.56%
Apta_1156	DNA	TTCTGGTGTGGTAACTCATGTATCGGTCGTGGGGGTCGTGGCATT	55.56%
Apta_650	DNA	GCACACCGATGGCGGTCCTGTTTAGGGGGTGTGCGTGGGGAGGTGCGGTTGC	53.85%
Apta_58	DNA	GCACCTTGATGACATGATAGTCGTTGTGTATGCAGTTGGC	53.33%
Apta_69	DNA	AGCGAGTAGGTTGGTGTGGTTGGGGCTCGCT	53.33%
Apta_70	DNA	AGGTCGTAGGTTGGTGTGGTTGGGGCGACCT	53.33%
Apta_75	DNA	CACTGGTAGGTTGGTGTGGTTGGGGCCAGTG	53.33%
Apta_590	DNA	ACCATCTGTGTAAGGGGTAAGGGGTGGGGGTGGGTACGTCT	53.33%
Apta_649	DNA	GCACACCGATGGCGGTCCTGTTTAGGGGGTGTGCGTG	53.33%
Apta_857	DNA	GCTATGGGTGGTCTGGTTGGGATTGGCCCCGGGAGCTGGC	53.33%
Apta_879	DNA	GGGGGGGTGAGACATCGGGCATGTTCATGGGTGGGGGGTA	53.33%
Apta_962	DNA	CCACACCGAGATGTTGTGACGGTTCCGATTGGCAAACTGA	53.33%
Apta_1065	DNA	CCTCGCTATGATGGAGTGCGTTTAGATCAGGGAACGGGTT	53.33%
Apta_1238	DNA	GGTTTTGTGTTTGTGTTTTGTGGTTGTTGT	53.33%
Apta_1255	DNA	GGCGCATGATCATTCGGTGGGTAAGGTGGTGGTAACGTTG	53.33%

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