AptaDB: Result

Interaction ID: 493

Aptamer sequence: CGACCAGGGTGGGAGGGGGACGGGCTGGCGGCGCGCGGTAATG

Target unique ID: Q7AP54

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_488	C7	Protein	HBP2_LISMO	1.46 μM	20 mM Tris-AcOH (pH 8.0), 100 mM NaCl, 200 mM KCl, 5 mM MgCl2, 0.5% Triton X-100, and 5% DMSO	22954361

Aptamer Sequence:	CGACCAGGGTGGGAGGGGGACGGGCTGGCGGCGCGCGGTAATG
The optimal secondary structure in dot-bracket notation:	...(((...)))........((.(((...))).))........
The centroid secondary structure in dot-bracket notation:	...........................................

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_488	Description	C7
Aptamer chemistry	DNA	Length	43 nt
GC content	76.7%	Molecular weight	13,537.63 Da
Molarity of 1 μg/μl solution	73.87 μM	Number of G-quadruplexes	3
G-Score	62	Function	Aptasensor
Sequence	CGACCAGGGTGGGAGGGGGACGGGCTGGCGGCGCGCGGTAATG
Applications	Peroxidase activity and strong binding affinity to hemin

Type	Detail	Type	Detail
Uniprot ID	Q7AP54	Protein name	Hemin/hemoglobin-binding protein 2 (Hn/Hb-binding protein 2) (P64) (Surface virulence-associated protein A)
Gene name(s)	hbp2 svpA lmo2185	Organism	Hemin/hemoglobin-binding protein 2 (Hn/Hb-binding protein 2) (P64) (Surface virulence-associated protein A)
Status	reviewed in uniprot database	Length	569
Mass	63,380	Activity regulation	Is overexpressed in mecA, clpC and clpP mutants
Involvement in disease	N/A	Catalytic activity	N/A
PDB ID(s)	4MYP;4NLA;
Function	Acts as an extracellular and cell wall-bound hemophore; scavenges host heme and hemoglobin from the environment and also serves as a cell wall receptor for both.

	Interaction ID	493
	Target type	Protein
	Target unique ID	Q7AP54
	Activity	1.46 μM
	Binding Conditions/Buffer	20 mM Tris-AcOH (pH 8.0), 100 mM NaCl, 200 mM KCl, 5 mM MgCl2, 0.5% Triton X-100, and 5% DMSO
	Assay	CD spectra of truncated aptamers (4 μM) in PB buffer without (black line) and with (red line) K+
	PubMed ID	22954361

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_1189	DNA	GCGGGCGGGGGGAGGGCGGCCGTGGGCTGCGAGTGGGAGG	62.79%
Apta_666	DNA	TCGACCTCGCGCGAGGAGGGTGGAGGGTCGTAGAGCGCGTA	60.47%
Apta_795	DNA	CGCAGCAAGGAGATTCGAGGGGGAAGGTTTGTTATAGGGGTTAATGGA	58.33%
Apta_565	DNA	GCGGGGTTGGGCGGGTGGGTTCGCTGGGCAGGGGGCGAGTG	58.14%
Apta_590	DNA	ACCATCTGTGTAAGGGGTAAGGGGTGGGGGTGGGTACGTCT	58.14%
Apta_869	DNA	ACTAGGACGCTTGGGAGGGGGGGTGGGGTGTCCGGTCGCG	58.14%
Apta_1013	DNA	ATCACGTATGGGGGGCCGGCATGAGGGCCGCGCGTGACA	58.14%
Apta_1014	DNA	TGGGGGTTGGGTGGATAGGCTGGCGTCGGGGCAGGTCAGT	58.14%
Apta_1081	DNA	CTTACGACTGTGGTCGGGTGGTGGGCCTCTAGAGGGGTGTCGTAAG	56.52%
Apta_46	DNA	CGGGTGGGAGTTGTGGGGGGGGGTGGGAGGGTT	55.81%
Apta_218	RNA	AGCCGCGAGGGGAGGGAUAGGGUAGGGCGCGGCU	55.81%
Apta_691	DNA	CGACGCGCGTTGGTGGTGGATGGTGTGTTACACGTGTTGT	55.81%
Apta_706	DNA	CCGGCCAAGGGTGGGAGGGAGGGGGCCGG	55.81%
Apta_830	DNA	GACGGTGGCAGGGAAAGGGGTCGGGCATATGGCGGAGGGG	55.81%
Apta_831	DNA	CCGCCCAGCGGGGGTAGGGCCGGACGTAGGAGGAGCTGCG	55.81%
Apta_1188	DNA	CGGGCGGGGCGTGGGGTGTTGGAGTGGAGGGCGGGGCGGC	55.81%
Apta_667	DNA	GCGGCGGTACCAGGTCAAAGGTGGGTGAGGGGACGCCAAGAGTCCCCGCGGTTACATAGAG	55.74%
Apta_255	DNA	TAGCGATACTGCGTGGGTTGGGGCGGGTAGGGCCAGCAGTCTCGT	55.56%
Apta_746	DNA	AGAGTGGGGGGGTGGGTGGATTTGACAGGTGGCATGCTGGAGAGT	55.56%
Apta_748	DNA	AGCTTGAGGGTGGGCGGGTGGACGCGGTAGTGGTATATAGGTCGG	55.56%

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