AptaDB: Result

Interaction ID: 832

Aptamer sequence: ATGCGAACAGGTGGGTGGGTTGGGTGGATTGTTCGGCTTCTTGAT

Target unique ID: CRM-CCL-185

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_750	S11.e	Cell	vaccinia virus–infected and–uninfected lung cancer A549 cells	46.2 nM	5 mmol MgCl2 and 4.5 g glucose into 1 L of Dulbecco's PBS without calcium and magnesium	19684903

Aptamer Sequence:	ATGCGAACAGGTGGGTGGGTTGGGTGGATTGTTCGGCTTCTTGAT
The optimal secondary structure in dot-bracket notation:	...(((((((..................)))))))..........
The centroid secondary structure in dot-bracket notation:	.......((((............................))))..

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_750	Description	S11.e
Aptamer chemistry	DNA	Length	45 nt
GC content	53.3%	Molecular weight	14,081.00 Da
Molarity of 1 μg/μl solution	71.02 μM	Number of G-quadruplexes	2
G-Score	21	Function	Target recognition
Sequence	ATGCGAACAGGTGGGTGGGTTGGGTGGATTGTTCGGCTTCTTGAT
Applications	Recognition of subtype non-small cell lung cancer

Type	Detail	Type	Detail
ATCC ID	CRM-CCL-185	Cell name	vaccinia virus-infected and-uninfected lung cancer A549 cells
Category	Human cells	Organism	Homo sapiens, human
Product type	Certified reference material	Cell type	epithelial cell
Morphology	epithelial	Tissue	Lung
Disease	Carcinoma	Product format	Frozen
Antigen expression	N/A	Genes expressed	N/A
Cell description	N/A
Application(s)	Assay development

	Interaction ID	832
	Target type	Cell
	Target unique ID	CRM-CCL-185
	Activity	46.2 nM
	Binding Conditions/Buffer	5 mmol MgCl2 and 4.5 g glucose into 1 L of Dulbecco's PBS without calcium and magnesium
	Assay	Binding assay of S11e to A549 cells
	PubMed ID	19684903

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_69	DNA	AGCGAGTAGGTTGGTGTGGTTGGGGCTCGCT	60.00%
Apta_482	DNA	AGCAAGGTTTGGTGTTGGTTGGTTGCTGGTTTTGGTTTC	60.00%
Apta_1205	DNA	GGCAGGTGGTGTTGGTTGGTTGTGCGTGGAGTTGG	60.00%
Apta_942	DNA	AGTGGGTAAGGTCTGGTGGATTGTGGACGGGGGGCGGGGCAGTGGCTTGA	58.00%
Apta_1071	DNA	TTGAAGTGACTCCGCACTGGGTGGGTGGGAGGGTCGTGCGGCTGGTCATAGCAGGGT	57.89%
Apta_255	DNA	TAGCGATACTGCGTGGGTTGGGGCGGGTAGGGCCAGCAGTCTCGT	57.78%
Apta_691	DNA	CGACGCGCGTTGGTGGTGGATGGTGTGTTACACGTGTTGT	57.78%
Apta_869	DNA	ACTAGGACGCTTGGGAGGGGGGGTGGGGTGTCCGGTCGCG	57.78%
Apta_609	DNA	GCTGCGAGGTGGGTGGGTGGGAGCAATTGGTCCTCGCTTAGCTTCTACGGTGGGCTATCT	56.67%
Apta_882	DNA	AATAGTCTGGTTCGGTGCTTGGTGCGATGTGTTAGTCAGACTCTTGTGTG	56.00%
Apta_1229	DNA	CACGGGCAGAGGGATAGGTTGTTGACGGGGCTGGTGGGTGGGTGCGCTCGCGCTATCGTGG	55.74%
Apta_41	DNA	CGGTCGCTCCGTGTGGCTTGGGTTGGGTGTGGCAGTGAC	55.56%
Apta_71	DNA	GGCGAGTAGGTTGGTGTGGTTGGGGCTCGCC	55.56%
Apta_72	DNA	AGTCAGTAGGTTGGTGTGGTTGGGGCTGACT	55.56%
Apta_74	DNA	GGACAGTAGGTTGGTGTGGTTGGGGCTGTCC	55.56%
Apta_266	DNA	TGGATGGTAGCTCGGTCGGGGTGGGTGGGTTGGCAAGTCT	55.56%
Apta_393	DNA	AGGAGGATAGTTCGGTGGCTGTTCAGGGTCTCCTCCT	55.56%
Apta_480	DNA	CGGCACACCTCGGTCGGTTTGTATGGCGAGCTGGTTTTTT	55.56%
Apta_782	DNA	AGGTGTGACTCGATCTGTGGGGGTTGGGGGGTGGTTTTTCGGAA	55.56%
Apta_858	DNA	GGCTTCAGGTTGGTCTGGTTGGGTTTGGCTCCTGTGTACG	55.56%

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