AptaDB: Result

Interaction ID: 828

Aptamer sequence: AGAGTGGGGGGGTGGGTGGATTTGACAGGTGGCATGCTGGAGAGT

Target unique ID: CRM-CCL-185

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_746	S11.a	Cell	vaccinia virus–infected and–uninfected lung cancer A549 cells	66.9 nM	5 mmol MgCl2 and 4.5 g glucose into 1 L of Dulbecco's PBS without calcium and magnesium	19684903

Aptamer Sequence:	AGAGTGGGGGGGTGGGTGGATTTGACAGGTGGCATGCTGGAGAGT
The optimal secondary structure in dot-bracket notation:	...................((((..(((........)))..))))
The centroid secondary structure in dot-bracket notation:	...................((((..(((........)))..))))

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_746	Description	S11.a
Aptamer chemistry	DNA	Length	45 nt
GC content	60.0%	Molecular weight	14,254.11 Da
Molarity of 1 μg/μl solution	70.16 μM	Number of G-quadruplexes	2
G-Score	21	Function	Target recognition
Sequence	AGAGTGGGGGGGTGGGTGGATTTGACAGGTGGCATGCTGGAGAGT
Applications	Recognition of subtype non-small cell lung cancer

Type	Detail	Type	Detail
ATCC ID	CRM-CCL-185	Cell name	vaccinia virus-infected and-uninfected lung cancer A549 cells
Category	Human cells	Organism	Homo sapiens, human
Product type	Certified reference material	Cell type	epithelial cell
Morphology	epithelial	Tissue	Lung
Disease	Carcinoma	Product format	Frozen
Antigen expression	N/A	Genes expressed	N/A
Cell description	N/A
Application(s)	Assay development

No relevant experimental diagram	Interaction ID	828
	Target type	Cell
	Target unique ID	CRM-CCL-185
	Activity	66.9 nM
	Binding Conditions/Buffer	5 mmol MgCl2 and 4.5 g glucose into 1 L of Dulbecco's PBS without calcium and magnesium
	Assay	N/A
	PubMed ID	19684903

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_565	DNA	GCGGGGTTGGGCGGGTGGGTTCGCTGGGCAGGGGGCGAGTG	62.22%
Apta_1256	DNA	GATCGGGTGTGGGTGGCGTAAAGGGAGCATCGGACA	62.22%
Apta_267	DNA	GAGTGCGTAATGGTACGATTTGGGAAGTGGCTTGGGGTGG	60.00%
Apta_942	DNA	AGTGGGTAAGGTCTGGTGGATTGTGGACGGGGGGCGGGGCAGTGGCTTGA	60.00%
Apta_1014	DNA	TGGGGGTTGGGTGGATAGGCTGGCGTCGGGGCAGGTCAGT	60.00%
Apta_245	DNA	ACAGGGGTGTGGGGACAGGGGTGTGGGG	57.78%
Apta_748	DNA	AGCTTGAGGGTGGGCGGGTGGACGCGGTAGTGGTATATAGGTCGG	57.78%
Apta_1189	DNA	GCGGGCGGGGGGAGGGCGGCCGTGGGCTGCGAGTGGGAGG	57.78%
Apta_979	DNA	ATGAGTAGTGCGGAGGGATGTGAATACTGACGCGGTCATAGTCGCTGTTGTACCATTGA	57.63%
Apta_597	DNA	TACTGGGCTGGGCTTGGATTAGATCACAGTGGAACATACAGGACACACG	57.14%
Apta_617	DNA	CAATGGGGTCGGGCGGGCCGGGTGTCATGGTGGACGGAGATGGGACGTAGAGGGCGGT	56.90%
Apta_940	DNA	TGTGCGGGGGAGGGGAGTGGCGCTGTTGGTGCGGGTATAGCGCGTGGTGT	56.00%
Apta_30	DNA	AGAGGGGGGGGTGGAGGGGGCTAGA	55.56%
Apta_263	DNA	AGAGGGGAGGGTCGGGTATCGGCGTGTTCGGGGGATCTGC	55.56%
Apta_364	DNA	TATGGTATGCTGTGTGGTATGGGGTGGCGTGCTCT	55.56%
Apta_391	DNA	ACCGTGGAGGATAGTTCGGTGGCTGTTCAGGGTCTCCTCCACGGT	55.56%
Apta_485	DNA	TGTCGTTGGGGGGGTAGGGAAGGGTGAAACGACAAGCCAGAAGTT	55.56%
Apta_488	DNA	CGACCAGGGTGGGAGGGGGACGGGCTGGCGGCGCGCGGTAATG	55.56%
Apta_580	DNA	ATGCGGGTATATGGTGCGAGAAAACAAGGGGCATACTGGA	55.56%
Apta_749	DNA	GATCGGTGGGTGGGGGGGTTGGAGATCATCCTCAGGGATTACGTC	55.56%

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