AptaDB: Result

Interaction ID: 391

Aptamer sequence: ACAGATACGGAACTACAGAGGTCAATTACGGTGGCCACGC

Target unique ID: P30990

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_385	P19	Protein	NEUT_HUMAN	0.37 nM	SHMCK buffer containing 0.1% (w/v) HSA, 0.2% (w/v) CHAPS, 0.04% (w/v) CHS, and 0.1% (w/v) LM	12054450

Aptamer Sequence:	ACAGATACGGAACTACAGAGGTCAATTACGGTGGCCACGC
The optimal secondary structure in dot-bracket notation:	...................(((((.......)))))....
The centroid secondary structure in dot-bracket notation:	...................(((((.......)))))....

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_385	Description	P19
Aptamer chemistry	DNA	Length	40 nt
GC content	52.5%	Molecular weight	12,347.97 Da
Molarity of 1 μg/μl solution	80.98 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Targeted binding
Sequence	ACAGATACGGAACTACAGAGGTCAATTACGGTGGCCACGC
Applications	Binding to GPCRs

Type	Detail	Type	Detail
Uniprot ID	P30990	Protein name	Neurotensin/neuromedin N [Cleaved into: Large neuromedin N (NmN-125); Neuromedin N (NN) (NmN); Neurotensin (NT); Tail peptide]
Gene name(s)	NTS	Organism	Neurotensin/neuromedin N [Cleaved into: Large neuromedin N (NmN-125); Neuromedin N (NN) (NmN); Neurotensin (NT); Tail peptide]
Status	reviewed in uniprot database	Length	170
Mass	19,795	Activity regulation	N/A
Involvement in disease	N/A	Catalytic activity	N/A
PDB ID(s)	2LNE;2LNF;2LNG;2LYW;2OYV;2OYW;3F6K;4PO7;5LUZ;6UP7;
Function	Neurotensin may play an endocrine or paracrine role in the regulation of fat metabolism.

	Interaction ID	391
	Target type	Protein
	Target unique ID	P30990
	Activity	0.37 nM
	Binding Conditions/Buffer	SHMCK buffer containing 0.1% (w/v) HSA, 0.2% (w/v) CHAPS, 0.04% (w/v) CHS, and 0.1% (w/v) LM
	Assay	Aptamer P19 interacts with the rat and human neurotensin receptor with high affinity. E. coli membrane nitrocellulose filter binding assay at 4°C. The interaction of P19 with the fully tagged rat (filled circles) and human (filled triangles) NTR is compared with that of the fully tagged human adenosine A2a receptor (filled squares); see solid lines throughout. The interaction of the negative control aptamer, G31, with the same three receptor fusion proteins is also shown and is denoted by the corresponding open symbols with a dashed line
	PubMed ID	12054450

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_972	DNA	TGACAGTACGTACTGAACTAATTTATAAGTAATTTGCTATGGTGGTATCC	56.00%
Apta_421	DNA	ATCTACGAATTCATCAGGGCTAAAGAGTGCAGAGTTACTTAGCCCGTGA	55.10%
Apta_257	DNA	GGCAGAAGAAATATCGAAACCCAGAATGGTCGGCCAGGCG	55.00%
Apta_675	DNA	ACTGTCCGTCGGGTTTAGGGTGGCGTTCGG	55.00%
Apta_975	DNA	AGACATAGTACATAACCGATCAATGAACATTTAATTAAATGGTGGTATCC	54.00%
Apta_976	DNA	GATGAATATCGCAATGAAATTATATATGTATAATTGGAACGGTGGTATCC	54.00%
Apta_1045	DNA	GGTGCTCAGGGAACTGTCTGAGGGATCAGGCTTAAGCCTGTCGAGCAGTT	54.00%
Apta_642	DNA	ATCCAATACCTCAGAACTCAGTTCGAGTCGTAAAGGGGAATCGCA	53.33%
Apta_158	DNA	CACAGGCTACGGCACGTAGAGCATCACCATGATCCTGTG	52.50%
Apta_306	DNA	CAAGGTAACCAGTACAAGGTGCTAAACGTAATGGCTTCG	52.50%
Apta_339	DNA	CACAGGCTACGGCACGTAGAGCATCACCATGATCCTGTGT	52.50%
Apta_343	DNA	AACAGAGGGACAAACGGGGGAAGATTTGACGTCGACGAC	52.50%
Apta_434	DNA	ACACGACGGGAATACTGACTCTCCCCCATGT	52.50%
Apta_462	DNA	GGTATAGACCCCTGAGTCCTACCGAGGGCACGGCA	52.50%
Apta_676	DNA	TCAGTCCCGATCAGGTGTGGGTGGCATTGG	52.50%
Apta_679	DNA	CAGGTGGCAGATCGGGTGTGGGTGGCCTGG	52.50%
Apta_4	DNA	CCTGCCTTTGGAACCGACCGCCAGGCCGAGGAAAGGCACCGACAGC	52.17%
Apta_556	DNA	GGCTCTCGGGACGACATGGATTTTCCATCAACGAAGTGCGTCCGTCCC	52.08%
Apta_971	DNA	TAGTCTAGGAACGGTACGATCGTATTCCAAGGACTTTGCTGGTGGTATCC	52.00%
Apta_1042	DNA	ATCACGATATGCCCGGCTCATCGGGGTTCAGTTGGGCGGTCACATGGAAA	52.00%

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