Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 815
Aptamer sequence: TAGGCAGTGGTTTGACGTCCGCATGTTGGGAATAGCCACGCCT
Target unique ID: CCL-119
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_733 | TE02 | Cell | Ramos Cells (B-cell lymphoma cell line) | 0.76 nM | 4.5 g/L glucose and 5 mM MgCl2 in Dulbecco's PBS | 17530817 |
Structure information of aptamer
| Aptamer Sequence: | TAGGCAGTGGTTTGACGTCCGCATGTTGGGAATAGCCACGCCT |
| The optimal secondary structure in dot-bracket notation: | .((((.((((((.....(((........)))..)))))))))) |
| The centroid secondary structure in dot-bracket notation: | .((((.((((((.....(((........)))..)))))))))) |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_733 | Description | TE02 |
| Aptamer chemistry | DNA | Length | 43 nt |
| GC content | 55.8% | Molecular weight | 13,290.52 Da |
| Molarity of 1 μg/μl solution | 75.24 μM | Number of G-quadruplexes | No QGRS found |
| G-Score | N/A | Function | Target recognition |
| Sequence | TAGGCAGTGGTTTGACGTCCGCATGTTGGGAATAGCCACGCCT | ||
| Applications | Specifically recognize the target lymphoma cells mixed with normal human bone marrow aspirates | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| ATCC ID | CCL-119 | Cell name | Ramos Cells (B-cell lymphoma cell line) |
| Category | Human cells | Organism | Homo sapiens, human |
| Product type | N/A | Cell type | T lymphoblast |
| Morphology | lymphoblast | Tissue | Peripheral blood |
| Disease | Acute lymphoblastic leukemia ALL | Product format | Frozen |
| Antigen expression | CD3; CD4; CD5; CD7; | Genes expressed | CD3; CD5; CD7; CD4 |
| Cell description | These CCRF-CEM human T lymphoblasts are isolated from the peripheral blood of a female, Caucasian 4 year old with acute lymphoblastic leukemia (ALL). The cell line can be used for immuno-oncology and immunology research. It is also a suitable transfection host. |
|
|
| Application(s) | 3D cell culture; Immune system disorder research; Immunology; Immuno-oncology | ||
Activity data
|
No relevant experimental diagram |
Interaction ID | 815 |
| Target type | Cell | |
| Target unique ID | CCL-119 | |
| Activity | 0.76 nM | |
| Binding Conditions/Buffer | 4.5 g/L glucose and 5 mM MgCl2 in Dulbecco's PBS |
|
| Assay | N/A |
|
| PubMed ID | 17530817 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_646 | DNA | TGGAGCTTGGATTGATGTGGTGTGTGAGTGCGGTGCCC | |
| Apta_62 | DNA | AGTCCGTGGTAGGGCAGGTTGGGGTGACT | |
| Apta_69 | DNA | AGCGAGTAGGTTGGTGTGGTTGGGGCTCGCT | |
| Apta_70 | DNA | AGGTCGTAGGTTGGTGTGGTTGGGGCGACCT | |
| Apta_71 | DNA | GGCGAGTAGGTTGGTGTGGTTGGGGCTCGCC | |
| Apta_72 | DNA | AGTCAGTAGGTTGGTGTGGTTGGGGCTGACT | |
| Apta_179 | DNA | TCGGTTGAGTGAGATTAGTCAAATGTTTAGAAGACAACCTC | |
| Apta_266 | DNA | TGGATGGTAGCTCGGTCGGGGTGGGTGGGTTGGCAAGTCT | |
| Apta_506 | DNA | AAGCGCGCGAGACTACGTTTGTAGGGATACCGATGTCG | |
| Apta_1093 | DNA | AGGAGGGTAGGTAGTGCTTGGTAGGGAAACTCCGCCGATT | |
| Apta_821 | DNA | CACAGTCACTCAGACGGCCGCTATTGTTGCCAGATTGCCTTTGGC | |
| Apta_1126 | DNA | TCGGGCGAGTCGTCTGCGGGCGGATAGACTACGACACCGCATCGTCCTCCC | |
| Apta_1127 | DNA | TCGGGCGAGTCGTCTGCGAGGCAACAAGCTCGTAATCCGCATCGTCCTCCC | |
| Apta_73 | DNA | GGGGAGTAGGTTGGTGTGGTTGGGGCTCCCC | |
| Apta_214 | DNA | AGGAAGGCTTTAGGTTTCCTAAAGCCTTCC | |
| Apta_364 | DNA | TATGGTATGCTGTGTGGTATGGGGTGGCGTGCTCT | |
| Apta_392 | DNA | GGATAGTTCGGTGGCTGTTCAGGGTCTCCTCCT | |
| Apta_393 | DNA | AGGAGGATAGTTCGGTGGCTGTTCAGGGTCTCCTCCT | |
| Apta_439 | DNA | TAACACTCGATTCTCCTAGCCCGCTAGAAATTCCCCTCCC | |
| Apta_912 | DNA | TAGTATGGACTTGTCTTCTTGTCAGAGTCTGGCAGCACGT |
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