Aptamer-target interaction descriptor
Aptamer-target interaction information
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_364 | B23 aptamer | Protein | LSHR_HUMAN | 321 nM | PBS | 30787284 |
Structure information of aptamer
| Aptamer Sequence: | TATGGTATGCTGTGTGGTATGGGGTGGCGTGCTCT |
| The optimal secondary structure in dot-bracket notation: | ((((.(((.(((((...))))).))).)))).... |
| The centroid secondary structure in dot-bracket notation: | ....(((((((..............)))))))... |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_364 | Description | B23 aptamer |
| Aptamer chemistry | DNA | Length | 35 nt |
| GC content | 54.3% | Molecular weight | 11,166.92 Da |
| Molarity of 1 μg/μl solution | 89.55 μM | Number of G-quadruplexes | 2 |
| G-Score | 19 | Function | Aptasensor |
| Sequence | TATGGTATGCTGTGTGGTATGGGGTGGCGTGCTCT | ||
| Applications | Real-time in vivo hormone monitoring | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Uniprot ID | P22888 | Protein name | Lutropin-choriogonadotropic hormone receptor (LH/CG-R) (Luteinizing hormone receptor) (LHR) (LSH-R) |
| Gene name(s) | LHCGR LCGR LGR2 LHRHR | Organism | Lutropin-choriogonadotropic hormone receptor (LH/CG-R) (Luteinizing hormone receptor) (LHR) (LSH-R) |
| Status | reviewed in uniprot database | Length | 699 |
| Mass | 78,643 | Activity regulation | N/A |
| Involvement in disease | Familial male precocious puberty (FMPP) | Catalytic activity | N/A |
| PDB ID(s) | 7FIG;7FIH;7FII;7FIJ; |
 |
Function | Receptor for lutropin-choriogonadotropic hormone. |
Activity data
 |
Interaction ID | 369 |
| Target type | Protein | |
| Target unique ID | P22888 | |
| Activity | 321 nM | |
| Binding Conditions/Buffer | PBS |
|
| Assay | Affinity study of the B23 aptamer. The concentration-response curve of B23 is shown in black. Two blue lines indicate the negative controls, one is the response of truncated B23 against FSH (red) and the other is the response of a random sequence against LH (blue). Means ± s.d. from three independent experiments are shown (n = 3) |
|
| PubMed ID | 30787284 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_1247 | DNA | GGTGGTTGCTGTTGTTTGTGGTTGGTGTGC | |
| Apta_266 | DNA | TGGATGGTAGCTCGGTCGGGGTGGGTGGGTTGGCAAGTCT | |
| Apta_1176 | DNA | AGTATGATATCGGTGTTTATGGTGTCTGTCTTCATACT | |
| Apta_22 | DNA | TGTGGGGGTGGACTGGGTGGGTACC | |
| Apta_25 | DNA | TGTGGGGGTGGTTGGGCGGGGCTGC | |
| Apta_69 | DNA | AGCGAGTAGGTTGGTGTGGTTGGGGCTCGCT | |
| Apta_70 | DNA | AGGTCGTAGGTTGGTGTGGTTGGGGCGACCT | |
| Apta_72 | DNA | AGTCAGTAGGTTGGTGTGGTTGGGGCTGACT | |
| Apta_74 | DNA | GGACAGTAGGTTGGTGTGGTTGGGGCTGTCC | |
| Apta_75 | DNA | CACTGGTAGGTTGGTGTGGTTGGGGCCAGTG | |
| Apta_350 | DNA | GGTGGTGGTGGTTGTGGTGGTGGTGG | |
| Apta_1085 | DNA | TGGGGTGGTGGTGTGGGTGGGGGTTTGTTC | |
| Apta_590 | DNA | ACCATCTGTGTAAGGGGTAAGGGGTGGGGGTGGGTACGTCT | |
| Apta_491 | DNA | TTGGGGTCTGCTCGGGATTGCGGAGAACGTGAATCT | |
| Apta_646 | DNA | TGGAGCTTGGATTGATGTGGTGTGTGAGTGCGGTGCCC | |
| Apta_255 | DNA | TAGCGATACTGCGTGGGTTGGGGCGGGTAGGGCCAGCAGTCTCGT | |
| Apta_752 | DNA | GCTATCTTATGGAAATTTCGTGTAGGGTTTGGTGTGGCGGGGCTA | |
| Apta_1156 | DNA | TTCTGGTGTGGTAACTCATGTATCGGTCGTGGGGGTCGTGGCATT | |
| Apta_1091 | DNA | CCATGGTAGGTATTGCTTGGTAGGGATAGTGGGCTTGATG | |
| Apta_11 | DNA | AGAGGGGGGGGTCGGGGTGGGTCGC |
DEV TOOL
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