Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 1125
Aptamer sequence: TTGAAGTGACTCCGCACTGGGTGGGTGGGAGGGTCGTGCGGCTGGTCATAGCAGGGT
Target unique ID: O46492
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_1071 | A10 | Protein | O46492_BOVIN | 11.7 nM | 150 mM NaCl, 20 mM HEPES, 1 mM KCl, 1 mM CaCl2, 1 mM MgCl2, pH 7.4 | 32561948 |
Structure information of aptamer
| Aptamer Sequence: | TTGAAGTGACTCCGCACTGGGTGGGTGGGAGGGTCGTGCGGCTGGTCATAGCAGGGT |
| The optimal secondary structure in dot-bracket notation: | (((..((((((((((((.((.............))))))))..))))))..)))... |
| The centroid secondary structure in dot-bracket notation: | (((..((((((((((((.(...............)))))))..))))))..)))... |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_1071 | Description | A10 |
| Aptamer chemistry | DNA | Length | 57 nt |
| GC content | 63.2% | Molecular weight | 17,849.37 Da |
| Molarity of 1 μg/μl solution | 56.02 μM | Number of G-quadruplexes | 3 |
| G-Score | 64 | Function | Target detection |
| Sequence | TTGAAGTGACTCCGCACTGGGTGGGTGGGAGGGTCGTGCGGCTGGTCATAGCAGGGT | ||
| Applications | Detection of bPAG4 in serum samples | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Uniprot ID | O46492 | Protein name | Pregnancy-associated glycoprotein 4 |
| Gene name(s) | PAG4 | Organism | Pregnancy-associated glycoprotein 4 |
| Status | unreviewed in uniprot database | Length | 380 |
| Mass | 42,663 | Activity regulation | N/A |
| Involvement in disease | N/A | Catalytic activity | N/A |
| PDB ID(s) | N/A |
 |
Function | N/A |
Activity data
 |
Interaction ID | 1125 |
| Target type | Protein | |
| Target unique ID | O46492 | |
| Activity | 11.7 nM | |
| Binding Conditions/Buffer | 150 mM NaCl, 20 mM HEPES, 1 mM KCl, 1 mM CaCl2, 1 mM MgCl2, pH 7.4 |
|
| Assay | Binding curves of aptamer to bPAG4-coated MB |
|
| PubMed ID | 32561948 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_464 | DNA | GTCAGATTCCACTATAGTAGGTTGGGTAGGGTGGTCGCAGTGGATGATATGTCGTAGGGG | |
| Apta_750 | DNA | ATGCGAACAGGTGGGTGGGTTGGGTGGATTGTTCGGCTTCTTGAT | |
| Apta_1090 | DNA | ATCCAGAGTGACGCAGCAGGGCTTGGGTTGGGAATAAGGATGTGGGAGGCGGCGAACATGGACACGGTGGCTTAGT | |
| Apta_1011 | DNA | GCTGTGTGACTCCTGCAATGGTAGCGGGTAGGGGAGGGAGGGTGAATGGAGGATGTTCATGGCAGCTGTATCTTGTCTCC | |
| Apta_1099 | DNA | ATCCATTGCCACTGACTACCAGCCGGGTGGGTGGGGGGGTTTGCTGGTATCGCATTGTGCGAAGTCAGTCGGTCGTTAGT | |
| Apta_255 | DNA | TAGCGATACTGCGTGGGTTGGGGCGGGTAGGGCCAGCAGTCTCGT | |
| Apta_782 | DNA | AGGTGTGACTCGATCTGTGGGGGTTGGGGGGTGGTTTTTCGGAA | |
| Apta_1044 | DNA | TTTAACGTAACCTGCAAGGCGGGGAGGTGCGAGCCCCGTGTGTGGCTTGC | |
| Apta_1047 | DNA | TAGTCCCAAGGCACATAAGGGCCGGAGGTCTAGCGTCAGGGATACAGAGA | |
| Apta_1072 | DNA | TTGAAGTGACTCCCACCCCGCCCCACCGTCCTTGCCACCGTTCCGCCATAGCAGGT | |
| Apta_1088 | DNA | ATCCAGAGTGACGCAGCATCGAGTGGCTTGTTTGTAATGTAGGGTTCCGGTCGTGGGTTGGACACGGTGGCTTAGT | |
| Apta_403 | DNA | GTCTTGACTAGTTACGCCCCCAAGAACCCGCTTGCCGTGGTGACGTTGATCATGTCGTTTGGTCATTCAGTTGGCGCCTC | |
| Apta_589 | DNA | GGGAATTCGAGCTCGGTACCATCTGTGTAAGGGGTAAGGGGTGGGGGTGGGTACGTCTAGCTGCAGGCATGCAAGCTTGG | |
| Apta_832 | DNA | CCGGAGGTGGGTGAGGTCTGCGGCAGGCTGTGTGGGTGGACCGGAGGGGGGTGAGGTCTGCGGCAGGCTGTGTGGGTGGA | |
| Apta_589 | DNA | GGGAATTCGAGCTCGGTACCATCTGTGTAAGGGGTAAGGGGTGGGGGTGGGTACGTCTAGCTGCAGGCATGCAAGCTTGG | |
| Apta_68 | DNA | AGATGCCTGTCGAGCATGCTGTGAATAGGTAGGGTCGGATGGGCTACGGTGTAGCTAAACTGCTTTGTCGACGGG | |
| Apta_266 | DNA | TGGATGGTAGCTCGGTCGGGGTGGGTGGGTTGGCAAGTCT | |
| Apta_666 | DNA | TCGACCTCGCGCGAGGAGGGTGGAGGGTCGTAGAGCGCGTA | |
| Apta_862 | DNA | TGTGCCAAAGAGAGTGGTGGGGGGGTGGGCGGAACTCGCG | |
| Apta_869 | DNA | ACTAGGACGCTTGGGAGGGGGGGTGGGGTGTCCGGTCGCG |
DEV TOOL
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