Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 584
Aptamer sequence: GGAUGUCCAGUCGCUUGCAAUGCCCUUUUAGACCCUGAUGAGCGGUGCUAGUUAGUUGCAGUUUCGGUUGUUACGCGAAACGGUGAAAGCCGUAGGUCU
Target unique ID: 134601
Aptamer ID Aptamer descriptor Target chemistry Target name Affinity Binding Conditions/Buffer PubMed ID
Apta_551 S3.asp.E4 Molecule Aspartame 5 mM 500 mM NaCl, 10 mM MgCl2, 1 mM EDTA, 10 mM sodium phosphate, 90 mM HEPES (pH 7.5) 15026535
Structure information of aptamer
Aptamer Sequence: GGAUGUCCAGUCGCUUGCAAUGCCCUUUUAGACCCUGAUGAGCGGUGCUAGUUAGUUGCAGUUUCGGUUGUUACGCGAAACGGUGAAAGCCGUAGGUCU
The optimal secondary structure in dot-bracket notation: ((....))....((((((..(((.((.(((((((((....)).))).))))..))..)))((((((((....)).))))))(((....)))))))))..
The centroid secondary structure in dot-bracket notation: ...............((((((......(((((((((....)).))).))))...))))))..((((((....)).))))..(((....)))........
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
Type Detail Type Detail
Aptamer ID Apta_551 Description S3.asp.E4
Aptamer chemistry RNA Length 99 nt
GC content 51.5% Molecular weight 31,836.74 Da
Molarity of 1 μg/μl solution 31.41 μM Number of G-quadruplexes No QGRS found
G-Score N/A Function Aptasensor
Sequence GGAUGUCCAGUCGCUUGCAAUGCCCUUUUAGACCCUGAUGAGCGGUGCUAGUUAGUUGCAGUUUCGGUUGUUACGCGAAACGGUGAAAGCCGUAGGUCU
Applications Detect caffeine or aspartame
Target information
Type Detail Type Detail
PubChem ID 134601 Molecular name Aspartame
Molecular formula C14H18N2O5 Molecular weight 294.3
IUPAC name (3S)-3-amino-4-[[(2S)-1-methoxy-1-oxo-3-phenylpropan-2-yl]amino]-4-oxobutanoic acid InChIKey IAOZJIPTCAWIRG-QWRGUYRKSA-N
LogP -0.311 Topological polar surface area 118.72
Hydrogen bond acceptor 5 Hydrogen bond donor 3

Synonym(s):
aspartame; 22839-47-0; Nutrasweet; Asp-phe-ome; Asp-Phe methyl ester; Aspartam; Canderel; L-Aspartyl-L-phenylalanine methyl ester; Aspartamo; Aspartamum; Aspartylphenylalanine methyl ester; Sweet dipeptide; Dipeptide sweetener; Methyl aspartylphenylalanate; H-Asp-Phe-OMe; 1-Methyl N-L-alpha-aspartyl-L-phenylalanate; Tri-sweet; methyl L-alpha-aspartyl-L-phenylalaninate; UNII-Z0H242BBR1; Equal; 3-Amino-N-(alpha-methoxycarbonylphenethyl) succinamic acid; (S)-3-Amino-4-(((S)-1-methoxy-1-oxo-3-phenylpropan-2-yl)amino)-4-oxobutanoic acid; CHEBI:2877; N-L-alpha-Aspartyl-L-phenylalanine 1-methyl ester; 3-Amino-N-(alpha-carboxyphenethyl)succinamic acid N-methyl ester; SC-18862; (3S)-3-amino-4-[[(2S)-1-methoxy-1-oxo-3-phenylpropan-2-yl]amino]-4-oxobutanoic acid; MFCD00002724; N-L-alpha-Aspartyl-L-phenylalanine methyl ester; Z0H242BBR1; (S)-3-amino-4-((S)-1-methoxy-1-oxo-3-phenylpropan-2-ylamino)-4-oxobutanoic acid; N-L-ALPHA-ASPARTYL L-PHENYLALANINE 1-METHYL ESTER; NCGC00091104-02; DSSTox_CID_107; E 951; DSSTox_RID_75371; DSSTox_GSID_20107; Sanecta; Aspartam [INN-French]; Aspartame, L,L-alpha-; Aspartamum [INN-Latin]; Aspartamo [INN-Spanish]; SMR000471870; CAS-22839-47-0; CCRIS 5456; SC 18862; HSDB 3915; Methyl L-aspartyl-L-phenylalanine; EINECS 245-261-3; Methyl L-alpha-aspartyl-L-phenylalanate; Aminosweet; L-Phenylalanine, N-L-alpha-aspartyl-, 1-methyl ester; Palsweet Diet; Methyl N-L-alpha-aspartyl-L-phenylalaninate; 1-Methyl N-L-alpha-aspartyl-L-phenylalanine; Pal Sweet; Aspartame [USAN:INN:BAN:NF]; NCGC00095160-01; DF-OMe; Aspartame (NF/INN); N-(L-a-Aspartyl)-L-phenylalanine methyl ester; Spectrum2_001706; Spectrum3_001949; Epitope ID:164026; 3-Amino-N-(alpha-carboxyphenethyl)succinamic acid N-methyl ester, stereoisomer; SCHEMBL3636; N-L-?-Aspartyl-L-phenylalanine 1-Methyl Ester; Succinamic acid, 3-amino-N-(alpha-carboxyphenethyl)-, N-methyl ester, stereoisomer; BSPBio_003549; MLS001066421; MLS001306461; Aspartame, analytical standard; SPECTRUM1505306; L-Phenylalanine, L-alpha-aspartyl-, 2-methyl ester; SPBio_001692; CHEMBL171679; DTXSID0020107; Asp-Phe methyl ester, >=98%; KBio3_002839; aspartyl-phenylalanine methyl ester; HMS1922B16; HMS2093B05; HMS2233D15; Pharmakon1600-01505306; HY-B0361; ZINC1532132; Tox21_111080; Tox21_111459; Tox21_202315; Tox21_302965; CCG-39444; NSC758953; s2036; AKOS015920055; L-Aspartyl-L-phenylalanyl methyl ester; Tert-butylN-(3-formylphenyl)carbamate; Tox21_111080_1; AM84801; Aspartame 1000 microg/mL in Methanol; DB00168; MCULE-7493691716; NSC 758953; NSC-758953; alpha-aspartyl-phenylalanine methyl ester; L-aspartyl-L-phenyl-alanine methyl ester; NCGC00091104-01; NCGC00091104-03; NCGC00091104-04; NCGC00091104-05; NCGC00095160-03; NCGC00256407-01; NCGC00259864-01; AC-12293; AS-13889; E951; L-Aspartyl-L-3-phenylalanine methyl ester; SBI-0206757.P001; AB0058040; Asp-Phe methyl ester, >=99.0% (HPLC); A0997; AM20060556; SW219179-1; alpha-L-Aspartyl-L-Phenylalanine Methyl Ester; L-Aspartyl-L-phenylalanine methyl ester, 96%; 39A470; D02381; AB00376622_08; AB00376622_09; A816383; N-(L-alpha-Aspartyl)-L-phenylalanine methyl ester; Q182040; SR-05000001682; J-502447; SR-05000001682-1; BRD-K78841970-001-06-2; Aspartame, European Pharmacopoeia (EP) Reference Standard; Aspartame, United States Pharmacopeia (USP) Reference Standard; (S)-3-amino-n-((s)-1-methoxycarbonyl-2-phenyl-ethyl)-succinamic acid; Aspartame, Pharmaceutical Secondary Standard; Certified Reference Material; (3S)-3-amino-3-{[(2S)-1-methoxy-1-oxo-3-phenylpropan-2-yl]carbamoyl}propanoic acid; 3-amino-4-[(1-carboxy-2-phenyl-ethyl)-methyl-amino]-4-oxo-butanoic acid;N-L-alpha-Aspartyl-L-phenylalanine Methyl Ester; 7421-84-3

Activity data
Interaction ID 584
Target type Molecule
Target unique ID 134601
Activity 5 mM
Binding Conditions/Buffer

500 mM NaCl, 10 mM MgCl2, 1 mM EDTA, 10 mM sodium phosphate, 90 mM HEPES (pH 7.5)
Assay

Specificity of the aspartame sensor S3.asp.E4
PubMed ID 15026535
Similar aptamers
Aptamer ID Aptamer chemistry Sequence Similarity
Apta_559 RNA GGAUGUCCAGUCGCUUGCAAUGCCCUUUUAGACCCUGAUGAGGAUCAUCGGACUUUGUCCUGUGGAGUAAGAUCGCGAAACGGUGAAAGCCGUAGGUCU
80.81%
Apta_613 RNA GGAUAAUAGCCGUAGGUUGCGAAAGCGACCCUGAUGAGCCUUAGGAUAUGCAUGAUGCAGAAGGACGUCGAAACGGUAGCGAGAGCUC 58.59%
Apta_561 RNA GGAUAAUAGCCGUAGGUUGCGAAAGCGACCCUGAUGAGCCUGUGGAAACAGACGUGGCACAUGACUACGUCGAAACGGUAGCGAGAGCUC 56.57%
Apta_566 RNA GGAUAAUAGCCGUAGGUUGCGAAAGCGACCCUGAUGAGCCCUGCGAUGCAGAAAGGUGCUGACGACACAUCGAAACGGUAGCGAGAGCUC 56.57%
Apta_710 RNA GGAUAAUAGCCGUAGGUUGCGAAAGCGACCCUGAUGAGUCUGGAUACCAUGCAUGAUGCACCUUGGCAGUCUUACGAAACGGUAGCGAGAGCUC 56.57%
Apta_294 RNA AUACCAGCUUAUUCAAUUGCCUGAUUAGCGGUAUCACGAUUACUUACCUUCGUUGCUGAGAUAGUAAGUGCAAUCU 55.56%
Apta_612 RNA GGGCGACCCUGAUGAGCCUUAGGAUAUGCUUCGGCAGAAGGACGUCGAAACGGUGAAAGCCGUAGGUUGCCC 55.56%
Apta_874 RNA GGAUAAUAGCCGUAGGUUGCGAAAGCGACCCUGAUGAGCCUUAGGAUAUGUCUGGAUACCAUGCAUGAUGCACCUUGGCAGUCUUACAGAAGGACGUCGAAACGGUAGCGAGAGCUC 55.56%
Apta_414 RNA GGGAGGACGAUGCGGCCGAUUAGAACUGGGCUGAGGCGUUCUGCAUUUCGGUGAUCAGACGACUCGCUGAGGAUCCGAGA 54.55%
Apta_429 RNA GCAUGCAGUGUCUAUUCUCGAGUAGCGAUCGUUGAAGGGGUAUAAGGUUGGCAGAUCGCUAGCAUGCAACUGACUCGGAUAAGCA 54.55%
Apta_562 RNA GGAUAAUAGCCGUAGGUUGCGAAAGCGACCCUGAUGAGCCUUUAGGGCCAAGUGUGGUGAAAGACACACUCGAAACGGUAGCGAGAGCUC 54.55%
Apta_299 RNA AUACCAGCUUAUUCAAUUGCCUGAAGACUGGAUAUACUCUUAAGCAUUUCUAUAAUCGAGAUAGUAAGUGCAAUCU 53.54%
Apta_500 RNA GGGAGAAAGGGAAGCUUGAGGUGCUAUGGAGUGGAGGAGUUGAAGGUGUCGGGGUUGGCAGAAGAAGGCGAGCGUACGGAUCCAUC 53.54%
Apta_719 RNA GGAAUGGAUCCACAUCUACGAAGGCUUUGAAGGUGAGACCGUGCAAAUGAGGAUGGUGUGGAUGAUUAGGGUUGUCGGUUUUCACUGCAGACUUGACGAAGCUU 52.88%
Apta_225 RNA GGGGCCACCAACGACAUUUAUCGAAUUGAUAACCUUACGCGAGAGCGUAGUUCGUUGAUAUAAAUAGUGCCCAUGGAUC 52.53%
Apta_298 RNA AUACCAGCUUAUUCAAUUGCCUGAAAACUGGUGUACCUCUUUGCCCUAUCUUAUCUGGAGAUAGUAAGUGCAAUCU 52.53%
Apta_410 RNA GGGAGAGCGGAAGCGUGCUGGGCCUGUCGUUCGCUGUGUCUUGCUACGUUACGUUACACGGUUGGCAUAACCCAGAGGUCGAUGG 52.53%
Apta_522 RNA GGGCGACCCUGAUGAGCACACGAGGGGGAAACCCCGGACAAUCAGACACGGUGUUCGAAACGGUGAAAGCCGUAGGUUGCCCUUU 52.53%
Apta_549 RNA GGAGCUCAGCCUUCACUGCAUGAUAAACCGAUGCUGGGCGAUUCUCCUGAAGUAGGGGAAGAGUUGUCAUGUAUGGGGGCACCACGGUCGGAUCCUG 52.53%
Apta_563 RNA GGAUAAUAGCCGUAGGUUGCGAAAGCGACCCUGAUGAGAAAGCCAAAGCCGUAGCGCAGAUGAUCUCGCCAUCAGUACCGAAACGGUAGCGAGAGCUC 52.53%

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AptaDB is an online database we developed to integrate comprehensive information about aptamer.
The detail of this database is described here:

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