AptaDB: Result

Interaction ID: 429

Aptamer sequence: TGGGAGCTGATGTCGCATGGGTTTTGATCACATGA

Target unique ID: P9WIN9

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_423	M64RA	Protein	MP64_MYCTU	N/A	20 mM HEPES, pH 7.35, 120 mM KCL, 1 mM CaCl2, and 1 mM MgCl2	19284297

Aptamer Sequence:	TGGGAGCTGATGTCGCATGGGTTTTGATCACATGA
The optimal secondary structure in dot-bracket notation:	..((((((.(((...))).))))))..........
The centroid secondary structure in dot-bracket notation:	..((((((.(((...))).))))))..........

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_423	Description	M64RA
Aptamer chemistry	DNA	Length	35 nt
GC content	48.6%	Molecular weight	10,872.99 Da
Molarity of 1 μg/μl solution	91.97 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Aptasensor
Sequence	TGGGAGCTGATGTCGCATGGGTTTTGATCACATGA
Applications	Detecting M. tuberculosis

Type	Detail	Type	Detail
Uniprot ID	P9WIN9	Protein name	Immunogenic protein MPT64 (Antigen MPT64)
Gene name(s)	mpt64 Rv1980c MTCY39.39	Organism	Immunogenic protein MPT64 (Antigen MPT64)
Status	reviewed in uniprot database	Length	228
Mass	24,855	Activity regulation	N/A
Involvement in disease	N/A	Catalytic activity	N/A
PDB ID(s)	2HHI;
Function	N/A

	Interaction ID	429
	Target type	Protein
	Target unique ID	P9WIN9
	Activity	N/A
	Binding Conditions/Buffer	20 mM HEPES, pH 7.35, 120 mM KCL, 1 mM CaCl2, and 1 mM MgCl2
	Assay	Map of the sandwich assay to detect the 77 samples. (A) Non-tuberculosis Mycobacterium and BCG Danish; (B) M. tuberculosis including H37Rv and 50 isolations, and M. bovis
	PubMed ID	19284297

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_1242	DNA	GGAGCGATTAGTAGTGGTCTTGTCTTTTGC	60.00%
Apta_41	DNA	CGGTCGCTCCGTGTGGCTTGGGTTGGGTGTGGCAGTGAC	58.97%
Apta_564	DNA	GGGGACTGTTGTGGTATTATTTTTGGTTGTGCAGT	57.14%
Apta_1236	DNA	GGACTGTTGTGGTATTATTTTTGGTTGTGC	57.14%
Apta_1246	DNA	GGAGATGTATGAGCGGGTGTTTGTGTTTGC	57.14%
Apta_646	DNA	TGGAGCTTGGATTGATGTGGTGTGTGAGTGCGGTGCCC	55.26%
Apta_183	DNA	TAGCACTGTGCGGCGCGTATTGTTCTAATCTGATTACATT	55.00%
Apta_1215	DNA	GTCGAATCAGCACCTCTGCATAGGTTACGTTTATACTGCG	55.00%
Apta_125	DNA	TGGTGGATGGCGCAGTCGGCGACAA	54.29%
Apta_1085	DNA	TGGGGTGGTGGTGTGGGTGGGGGTTTGTTC	54.29%
Apta_1205	DNA	GGCAGGTGGTGTTGGTTGGTTGTGCGTGGAGTTGG	54.29%
Apta_750	DNA	ATGCGAACAGGTGGGTGGGTTGGGTGGATTGTTCGGCTTCTTGAT	53.33%
Apta_910	DNA	TCACGTGCATGATAGACGGCGAAGCCGTCGAGTTGCTGTGTGCCGATGCACGTGA	52.73%
Apta_58	DNA	GCACCTTGATGACATGATAGTCGTTGTGTATGCAGTTGGC	52.50%
Apta_165	DNA	TGGACGAACTGCCCTCAGCTACTTTCATGTTGCTGACGCA	52.50%
Apta_266	DNA	TGGATGGTAGCTCGGTCGGGGTGGGTGGGTTGGCAAGTCT	52.50%
Apta_375	DNA	TTGGGGAGGGGCACTGGGCAGTGTAATTTACGAAAGCGAG	52.50%
Apta_445	DNA	AGCCATGACGATGTCGTTACGTAGATGTAGAGACTCCTAA	52.50%
Apta_507	DNA	GGCGGGGCTACGAAGTAGTGATTTTTTCCGATGGCCCGTG	52.50%
Apta_691	DNA	CGACGCGCGTTGGTGGTGGATGGTGTGTTACACGTGTTGT	52.50%

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