Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 410
Aptamer sequence: GTCTTGACTAGTTACGCCCACAAGAACCTGCTTGCCATGGTGACGCCGATCATGCTTTTTGGTCATTCAGTTGGCGCCTC
Target unique ID: P48551
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_404 | IFNα-3 aptamer | Protein | INAR2_HUMAN | 3.96 nM | 10 mM Tris pH 7.5, 10 mM MgCl2, 50 mM KCl, 25 mM NaCl | 33063508 |
Structure information of aptamer
| Aptamer Sequence: | GTCTTGACTAGTTACGCCCACAAGAACCTGCTTGCCATGGTGACGCCGATCATGCTTTTTGGTCATTCAGTTGGCGCCTC |
| The optimal secondary structure in dot-bracket notation: | ..............((((..((((......))))....)))).(((((((.(((((....)).)))...))))))).... |
| The centroid secondary structure in dot-bracket notation: | ...............(....((((......))))......)..(((((((.(((((....)).)))...))))))).... |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_404 | Description | IFNα-3 aptamer |
| Aptamer chemistry | DNA | Length | 80 nt |
| GC content | 52.5% | Molecular weight | 24,529.66 Da |
| Molarity of 1 μg/μl solution | 40.77 μM | Number of G-quadruplexes | No QGRS found |
| G-Score | N/A | Function | Targeted therapy |
| Sequence | GTCTTGACTAGTTACGCCCACAAGAACCTGCTTGCCATGGTGACGCCGATCATGCTTTTTGGTCATTCAGTTGGCGCCTC | ||
| Applications | Designing of aptamers similar to the IFNα-3 aptamer provides a novel approach to assess other inflammatory protein(s) in patients before, during, and after completion of treatment and would denote clinical improvement in successfully treated patients | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Uniprot ID | P48551 | Protein name | Interferon alpha/beta receptor 2 (IFN-R-2) (IFN-alpha binding protein) (IFN-alpha/beta receptor 2) (Interferon alpha binding protein) (Type I interferon receptor 2) |
| Gene name(s) | IFNAR2 IFNABR IFNARB | Organism | Interferon alpha/beta receptor 2 (IFN-R-2) (IFN-alpha binding protein) (IFN-alpha/beta receptor 2) (Interferon alpha binding protein) (Type I interferon receptor 2) |
| Status | reviewed in uniprot database | Length | 515 |
| Mass | 57,759 | Activity regulation | N/A |
| Involvement in disease | Immunodeficiency 45 (IMD45) | Catalytic activity | N/A |
| PDB ID(s) | 1N6U;1N6V;2HYM;2KZ1;2LAG;3S8W;3S9D;3SE3;3SE4; |
 |
Function | Associates with IFNAR1 to form the plasma membrane receptor in the type I interferon signaling pathway. |
Activity data
 |
Interaction ID | 410 |
| Target type | Protein | |
| Target unique ID | P48551 | |
| Activity | 3.96 nM | |
| Binding Conditions/Buffer | 10 mM Tris pH 7.5, 10 mM MgCl2, 50 mM KCl, 25 mM NaCl |
|
| Assay | Binding affinity of selected aptamers. The dissociation constant (Kd) curve derived through nonlinear regression represents the binding affinity of (C) IFNα-1 and (D) IFNα-3 aptamers to 250 ng of the IFN-α protein |
|
| PubMed ID | 33063508 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_403 | DNA | GTCTTGACTAGTTACGCCCCCAAGAACCCGCTTGCCGTGGTGACGTTGATCATGTCGTTTGGTCATTCAGTTGGCGCCTC | |
| Apta_1000 | DNA | ATAGGAGTCGACCGACCAGAACCGTTCCCATACTTCTCCAGCGATACTTAGTGCGTCTACATCTAGACTC | |
| Apta_570 | DNA | GGCAAGATGTGCCCAGAACAGTTGCTTGTATGGTGGGGAGCGTCCATATTGGCTTAAAC | |
| Apta_1071 | DNA | TTGAAGTGACTCCGCACTGGGTGGGTGGGAGGGTCGTGCGGCTGGTCATAGCAGGGT | |
| Apta_1173 | DNA | TTGACTTGCCACTGACTACCCCAGTATCTCTTCAACGCTATGGTGCATGTACTACGTATTGAAGTCAGTCGGTCGTCATC | |
| Apta_600 | DNA | GTCTCTGTGTGCGCCAGAGAACACTGGGGCAGATATGGGCCAGCACAGAATGAGGCCC | |
| Apta_661 | DNA | GGACAACATAGGAAAAAGGCTCTGCTACCGGATCCCTGTTGTATGGGCATATCTGTTGAT | |
| Apta_738 | DNA | ATCGTCTGCTCCGTCCAATACCTGCATATACACTTTGCATGTGGTTTGGTGTGAGGTCGTGC | |
| Apta_740 | DNA | ATCGTCTGCTCCGTCCAATAAACCTGCATAATTTATAAGTCTAGACTGCTGCATTTGGTGTGAGGTCGTGC | |
| Apta_600 | DNA | GTCTCTGTGTGCGCCAGAGAACACTGGGGCAGATATGGGCCAGCACAGAATGAGGCCC | |
| Apta_704 | DNA | GTACGAATTCACGAGGTTGCCAGCGGGGCCAGCCACTTCTGTCAGTGAATTCCTGCTCGTATATCTACTCGCCCGCCTGCGAGCATGGAGTCGGATCCTCTA | |
| Apta_736 | DNA | ATCGTCTGCTCCGTCCAATATGATGACACCTGCATAATTTATAGTGAGTCTTGATTCACGCTGCATTTGGTGTGAGGTCGTGC | |
| Apta_742 | DNA | ATCGTCTGCTCCGTCCAATAGGACCGATAGGAACCACGGACTGCATGTTTCTGCATTTGACGTGGTTTGGTGTGAGGTCGTGC | |
| Apta_127 | DNA | GGTATTGAGGGTCGCATCCACTGGTCGTTGTTGTCTGTTGTCTGTTATGTTGTTTCGTGATGGCTCTAACTCTCCTCT | |
| Apta_618 | DNA | GCGGCAAATCAGCATACGAGTGTACAGGGACGGGACGGGTGGGTAAAAGGTGTCGCCTCAG | |
| Apta_728 | DNA | CGACCCGGCACAAACCCAGAACCATATACACGATCATTAGTCTCCTGGGCCG | |
| Apta_737 | DNA | ATCGTCTGCTCCGTCCAATAGTGCATTGAAACTTCTGCATCCTCGTTTGGTGTGAGGTCGTGC | |
| Apta_867 | DNA | CTCCTCTGACTGTAACCACGCCACCGAGCCTACCACATGTGACATCCCAGGACATAGCTGGCATAGGTAGTCCAGAAGCC | |
| Apta_923 | DNA | ATCCAGAGTGACGCAGCATCGACCTCTGTGCGAGCGGGTTGCATATATATTTTGCATGGACACGGTGGCTTAGT | |
| Apta_931 | DNA | ATACGACTCACTATTAGGGACCACATAAACATGCATCGCTCAATAACGGGATATTGTTGTGCTAGACTACTGACTACAA |
DEV TOOL
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