Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 478
Aptamer sequence: CCCTGCACTCGCAATGGCCCCTCTACACCACAAACGGACA
Target unique ID: Q61727
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_473 | R5Bw2 | Protein | I10R1_MOUSE | >200 nM | 150 mmol/L NaCl, 20 mmol/L KCl, and 2 mmol/L HEPES heated to 82 °C and slowly cooling to room temperature | 22434135 |
Structure information of aptamer
| Aptamer Sequence: | CCCTGCACTCGCAATGGCCCCTCTACACCACAAACGGACA |
| The optimal secondary structure in dot-bracket notation: | .(((((....)))..))..........((......))... |
| The centroid secondary structure in dot-bracket notation: | ...(((....)))........................... |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_473 | Description | R5Bw2 |
| Aptamer chemistry | DNA | Length | 40 nt |
| GC content | 60.0% | Molecular weight | 12,084.79 Da |
| Molarity of 1 μg/μl solution | 82.75 μM | Number of G-quadruplexes | No QGRS found |
| G-Score | N/A | Function | Targeted therapy |
| Sequence | CCCTGCACTCGCAATGGCCCCTCTACACCACAAACGGACA | ||
| Applications | Bound to IL-10 receptor on the cell surface and blocked IL-10 function in vitro | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Uniprot ID | Q61727 | Protein name | Interleukin-10 receptor subunit alpha (IL-10 receptor subunit alpha) (IL-10R subunit alpha) (IL-10RA) (CDw210a) (Interleukin-10 receptor subunit 1) (IL-10R subunit 1) (IL-10R1) (CD antigen CD210) |
| Gene name(s) | Il10ra Il10r | Organism | Interleukin-10 receptor subunit alpha (IL-10 receptor subunit alpha) (IL-10R subunit alpha) (IL-10RA) (CDw210a) (Interleukin-10 receptor subunit 1) (IL-10R subunit 1) (IL-10R1) (CD antigen CD210) |
| Status | reviewed in uniprot database | Length | 575 |
| Mass | 64,248 | Activity regulation | N/A |
| Involvement in disease | N/A | Catalytic activity | N/A |
| PDB ID(s) | N/A |
 |
Function | Cell surface receptor for the cytokine IL10 that participates in IL10-mediated anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. |
Activity data
|
No relevant experimental diagram |
Interaction ID | 478 |
| Target type | Protein | |
| Target unique ID | Q61727 | |
| Activity | >200 nM | |
| Binding Conditions/Buffer | 150 mmol/L NaCl, 20 mmol/L KCl, and 2 mmol/L HEPES heated to 82 °C and slowly cooling to room temperature |
|
| Assay | N/A |
|
| PubMed ID | 22434135 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_472 | DNA | CCCCCACATTCAAATGGCCCCCCTACTCAATTACTGCACA | |
| Apta_253 | DNA | CCCTGTTCGCTATCCCCCATCCCCCGATTGC | |
| Apta_159 | DNA | GCGTGCAGCATGACCACCGATGTCACACTTAATGAGAAT | |
| Apta_161 | DNA | GTGTGCAGATCTGATTGCCTGCGTCAACCTACATACAAC | |
| Apta_163 | DNA | GCGCATGACCATTGATGTCTGCACCATCAGAGGCAGAG | |
| Apta_436 | DNA | CCCCCGCATCACGCCGTGGTGCGATTGACACAATTGCAAT | |
| Apta_872 | DNA | ACCGCCTTCCACCGTTCTCCACCACCCCTCAAACAACCCT | |
| Apta_1023 | DNA | CTCCTTCAACGAAGTGGGTCTCCTTCAACGAAGTGGGTCTC | |
| Apta_687 | DNA | GGGACAGCGGAGGTCTCCCACCCACCGGCCCCTGCGGCACACCAACCTGTATGGATGCG | |
| Apta_4 | DNA | CCTGCCTTTGGAACCGACCGCCAGGCCGAGGAAAGGCACCGACAGC | |
| Apta_160 | DNA | CGGTGCAGTGGATACATGCCAGCCGTAGCCATCGTGGATA | |
| Apta_251 | DNA | GCCCTACATTCTGTGCGACTCCCCCCCCCGC | |
| Apta_252 | DNA | CCCTTACCTACTCTGCTCCCCCCCCCCCTCGGCTGC | |
| Apta_325 | DNA | TACTGCATGCACACCACTTCAACTA | |
| Apta_325 | DNA | TACTGCATGCACACCACTTCAACTA | |
| Apta_441 | DNA | TGAATCTCGCGCTCGTTGGTACCCTTAAAATAAAGGCATA | |
| Apta_448 | DNA | TAACTCACTCCATACTCACTTGCTGATTCGCCAACAACACACCCTTAAACAGTCCC | |
| Apta_514 | DNA | ACTCAGCACGCGTGTAGGGAAAACCAACCGCCCACAAGCTGCATGCAACG | |
| Apta_604 | DNA | TCCTGACCTTAGCCTATGTTTTCTCTACCGGCTATGTTAC | |
| Apta_694 | DNA | ACTCACTATGGAAGAGATGGCGACATCTCTTCTCCGAGCCGGTCGAAATAGTGAGT |
DEV TOOL
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