Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 40
Aptamer sequence: UGUUCAUGUCCUACUGUUCAAACAAAAAAACUGUGCACAAAAAUAAAUUGGGGCAUGGACA
Target unique ID: O43504
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_40 | A9 | Protein | LTOR5_HUMAN | N/A | 0.1 M sodium phosphate (pH 7.4), 150 mM NaCl, 20 mM imidazol, 0.1% (v/v) NP-40, 100 mg/ml yeast tRNA, 30 °C | 22125633 |
Structure information of aptamer
| Aptamer Sequence: | UGUUCAUGUCCUACUGUUCAAACAAAAAAACUGUGCACAAAAAUAAAUUGGGGCAUGGACA |
| The optimal secondary structure in dot-bracket notation: | (((((((((((((.((((...(((.......))).......))))...))))))))))))) |
| The centroid secondary structure in dot-bracket notation: | (((((((((((((.........(.........)...............))))))))))))) |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_40 | Description | A9 |
| Aptamer chemistry | RNA | Length | 61 nt |
| GC content | 36.1% | Molecular weight | 18,820.16 Da |
| Molarity of 1 μg/μl solution | 53.13 μM | Number of G-quadruplexes | No QGRS found |
| G-Score | N/A | Function | Targeted binding |
| Sequence | UGUUCAUGUCCUACUGUUCAAACAAAAAAACUGUGCACAAAAAUAAAUUGGGGCAUGGACA | ||
| Applications | Suppressed pgRNA packaging and DNA synthesis | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Uniprot ID | O43504 | Protein name | Ragulator complex protein LAMTOR5 (Hepatitis B virus X-interacting protein) (HBV X-interacting protein) (HBX-interacting protein) (Late endosomal/lysosomal adaptor and MAPK and MTOR activator 5) |
| Gene name(s) | LAMTOR5 HBXIP XIP | Organism | Ragulator complex protein LAMTOR5 (Hepatitis B virus X-interacting protein) (HBV X-interacting protein) (HBX-interacting protein) (Late endosomal/lysosomal adaptor and MAPK and MTOR activator 5) |
| Status | reviewed in uniprot database | Length | 91 |
| Mass | 9,614 | Activity regulation | N/A |
| Involvement in disease | N/A | Catalytic activity | N/A |
| PDB ID(s) | 3MS6;3MSH;5VOK;5X6U;5X6V;5Y38;5Y39;5Y3A;5YK3;6EHP;6EHR;6NZD;6U62;6ULG;7T3A;7T3B;7T3C; |
 |
Function | As part of the Ragulator complex it is involved in amino acid sensing and activation of mTORC1, a signaling complex promoting cell growth in response to growth factors, energy levels, and amino acids. |
Activity data
|
No relevant experimental diagram |
Interaction ID | 40 |
| Target type | Protein | |
| Target unique ID | O43504 | |
| Activity | N/A | |
| Binding Conditions/Buffer | 0.1 M sodium phosphate (pH 7.4), 150 mM NaCl, 20 mM imidazol, 0.1% (v/v) NP-40, 100 mg/ml yeast tRNA, 30 °C |
|
| Assay | N/A |
|
| PubMed ID | 22125633 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_1123 | RNA | AUUCGGGCCCAGGAACCAACUAUAUAAAUGUCCCGAAUGCUUCGACG | |
| Apta_225 | RNA | GGGGCCACCAACGACAUUUAUCGAAUUGAUAACCUUACGCGAGAGCGUAGUUCGUUGAUAUAAAUAGUGCCCAUGGAUC | |
| Apta_1056 | RNA | GUCAUGUUGUGUUGUGGUCCAUCCUUUUCUACGCCAGUCGAGUCUGUAGCGUGGGAGUAAACUGAACGGUCAGACAGGA | |
| Apta_157 | RNA | GGGUUCACUGCAGACUUGACGAAGCUUACAAACAAGAGCAAAAAGGGAGUUGACGUAGACUGUGCGGAAUGGAUCCACAUCUACGAAUUC | |
| Apta_153 | RNA | CCACAGUUCAGAGGCACCGCGAACAAAACGCAAGACAGAGUGCCGACAAGAAC | |
| Apta_425 | RNA | UGCCGCCAUAUCACACGGAUUUAAUCGCCGUAGAAAAGCAUGUCAAAGCCG | |
| Apta_1122 | RNA | ACAACCCGGAACAACGUCUAACAGUGUACCAUAACCCGGCAUUCA | |
| Apta_1142 | RNA | ACGAGUCACACGUUGAUGACUGGAUGGUAGUUAAAGAGGGUGGGGCAACAA | |
| Apta_1054 | RNA | CUGACGUUUCGAACGUCAAAUGCGGAAGUUGUCGGAGCUAAGAAACGGGUAAUGAUGCCGUUGAUGUGAAGGCGGGCUCA | |
| Apta_690 | RNA | GGGCGAAUUCCCGAGGACCAAAUAGUACCACCCGGGAAAACAGCUAAUGCCGAAACGGAGAUUUUUUCUGCAGAAGCU | |
| Apta_59 | RNA | GGACCUCGGCGAAAGCUAACGUCUCAUGGCUAAAUUGCCAUGUUGCUACAAAUGAUAUGACUAGAGAGGUUAGGUGCCUCGUGAUGUCCAGUCGC | |
| Apta_80 | RNA | GCAAUGGUACGGUACUUCCUUUGGAAGAUAGCUGGAGAACUAACCAAAAGUGCACGCUACUUUGCUAA | |
| Apta_885 | RNA | GGGAGAGCGGAAGCGUGCUGGGCCUGGUUGCUGUAAAAAUAGACACGAAUCUGCCGACCAUAACCCAGAGGUCGAUGGAUCCCCCC | |
| Apta_557 | RNA | GGAACACUAUCCGAUGGCACCGACCAUAGGCUCGGGUUGCCAGAGGUUCCACACUUUCAUCGAAAAGCCUAUGCUAGGCAAUGACAUGGACUCCUUGGUCAUUAGGAUCG | |
| Apta_83 | DNA | GGCTTTACACTTTATGCTTCCGTAAAAACACAAACACACGCACATACATAAAATAACAATTTCACACAGGAAACAG | |
| Apta_296 | RNA | AUACCAGCUUAUUCAAUUGCCUGAAAACCUGGUCUCUCUGUCAGCAAAGACUAUGTUGAGAUAGUAAGUGCAAUCU | |
| Apta_299 | RNA | AUACCAGCUUAUUCAAUUGCCUGAAGACUGGAUAUACUCUUAAGCAUUUCUAUAAUCGAGAUAGUAAGUGCAAUCU | |
| Apta_85 | RNA | UGCGACUGUUAUAACCUAACAGCGACGUAAAGAUA | |
| Apta_136 | RNA | CGUGUCGUCCUGGUACGAUUUGGUAUAUAACCGUGGCUUUUCGACA | |
| Apta_269 | RNA | AACGCUCAACGUCAGAAGGCAACGUAUAGGCAAGCACACUUCGACAUGAGGC |
DEV TOOL
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