Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 885
Aptamer sequence: TGCGTGTGTAGTGTGTCTGCATGCCCCTGTAATCGCCCATGGGTAGCCTCTTAGGGATTTGGGCGG
Target unique ID: PCS-500-012
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_801 | HM69 | Cell | Mesenchymal stem cells (MSCs) | 9.67 nM | 157 mM Na+, 140 mM Cl-, 4.45 mM K+, 10.1 mM HPO42-, 1.76 mM H2PO4- | 31806966 |
Structure information of aptamer
| Aptamer Sequence: | TGCGTGTGTAGTGTGTCTGCATGCCCCTGTAATCGCCCATGGGTAGCCTCTTAGGGATTTGGGCGG |
| The optimal secondary structure in dot-bracket notation: | ...((((((((.....)))))))).........((((((.......(((...)))....)))))). |
| The centroid secondary structure in dot-bracket notation: | ...((((((((.....)))))))).........((((((.......(((...)))....)))))). |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_801 | Description | HM69 |
| Aptamer chemistry | DNA | Length | 66 nt |
| GC content | 57.6% | Molecular weight | 20,437.02 Da |
| Molarity of 1 μg/μl solution | 48.93 μM | Number of G-quadruplexes | No QGRS found |
| G-Score | N/A | Function | Targeted therapy |
| Sequence | TGCGTGTGTAGTGTGTCTGCATGCCCCTGTAATCGCCCATGGGTAGCCTCTTAGGGATTTGGGCGG | ||
| Applications | Enhances bone defect repair by improving stem cell recruitment | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| ATCC ID | PCS-500-012 | Cell name | Mesenchymal stem cells (MSCs) |
| Category | Human cells | Organism | Homo sapiens, human |
| Product type | N/A | Cell type | mesenchymal stem cell |
| Morphology | spindle shaped, fibroblast-like | Tissue | Bone |
| Disease | Normal | Product format | Frozen |
| Antigen expression | N/A | Genes expressed | N/A |
| Cell description | One vial of Bone Marrow-Derived Mesenchymal Stem Cells; Normal, Human containing a minimum of 1 x 10**6 viable cells (provided). |
|
|
| Application(s) | Stem cell research | ||
Activity data
 |
Interaction ID | 885 |
| Target type | Cell | |
| Target unique ID | PCS-500-012 | |
| Activity | 9.67 nM | |
| Binding Conditions/Buffer | 157 mM Na+, 140 mM Cl-, 4.45 mM K+, 10.1 mM HPO42-, 1.76 mM H2PO4- |
|
| Assay | Flow cytometry evaluation of the binding affinities to MSCs by FITC-labeled aptamers 69 |
|
| PubMed ID | 31806966 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_223 | DNA | CATTGAGATAGCTAGTTGTAGCTGCGTCATAGGCTGGGTTGGGTCTAGTGGTTGGGTGTG | |
| Apta_803 | DNA | ATGAGAAGCGTCGGTGTGGTTACTCCGGGCACTTGATATATCGATATGGAGAATAATGCACCCTGAGCGGGCTGGCAAGGCGCATA | |
| Apta_1046 | DNA | TCGCATGGAGGGCAGAGCCGCCTGCCGGGATCCGGCCCTCTTGGGCGGGC | |
| Apta_461 | DNA | CGCTCGGCTTCACGAGATTCGTGTCGTTGTGTCCTGTACCCGCCTTGACCAGTCACTCTAGAGCATCCGGACTG | |
| Apta_1175 | DNA | ATGAGAGCGTCGGTGTGGTATCCTACTCGTGATATCGGTGTTTATGGTGTCTGTCTTCATGTAGGAGGGTGCGGAAGTA | |
| Apta_33 | DNA | GCGGTAAACTGCGTCCGAAGGGGCTGCAGTGACCCGAATGGGTCCGCCGCGAAGCG | |
| Apta_608 | DNA | TGAGGCGGGTGGGTGGGTTGAATACGCTGATCACCCCATCGGAGAACGTTAAGGCGCTTT | |
| Apta_817 | DNA | TGCGTGAGCGGTAGGCCCCTACGACCCACTGTGGTTGGGC | |
| Apta_1062 | DNA | GGGGTCTTGCAGGTCCCGTAGGAGGGGCCATTGGAGTGGGG | |
| Apta_1156 | DNA | TTCTGGTGTGGTAACTCATGTATCGGTCGTGGGGGTCGTGGCATT | |
| Apta_1272 | DNA | TCCGTGTATAGGTCGGGTCTCTTGCCAACTGATTCGTTGAAAAGTATAGCCCCGCAGGG | |
| Apta_1109 | DNA | GGTATGTGTCGTTGATCTGGATATCTATGCCTAATGCTCGTAGGCGACTGTTCGGATAGTAACGTTGACCGCATAC | |
| Apta_832 | DNA | CCGGAGGTGGGTGAGGTCTGCGGCAGGCTGTGTGGGTGGACCGGAGGGGGGTGAGGTCTGCGGCAGGCTGTGTGGGTGGA | |
| Apta_1234 | DNA | TGTACCGTCTGAGCGATTCGTACTGTGGGCACCAATCGTACCCAATACTTGCGAATCAGCCAGTCAGTGTTAAGGAGTGC | |
| Apta_68 | DNA | AGATGCCTGTCGAGCATGCTGTGAATAGGTAGGGTCGGATGGGCTACGGTGTAGCTAAACTGCTTTGTCGACGGG | |
| Apta_553 | DNA | TGTACCGTCTGAGCGATTCGTACGAACGGCTTTGTACTGTTTGCACTGGCGGATTTAGCCAGTCAGTGTTAAGGAGTGC | |
| Apta_57 | DNA | GCGGGTCGGTTGCTCGCTTCGCCCGATCGGTCTAAGGGTG | |
| Apta_344 | DNA | CACTACAGAGGTTGCGTCTGTCCCACGTTGTCATGGGGGGTTGGCCTG | |
| Apta_607 | DNA | TGAGGCGGGTGGGTGGGTTGAATATGCTGATTACCCCATCGGAGAACGTTAAGGCGCTTC | |
| Apta_610 | DNA | GCTGTGAGGTGGGTGGGTGGGAGCAATTGGTCCTCGCTTAGCTTCTACGGTGGGCTATCT |
DEV TOOL
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