Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 1054
Aptamer sequence: TAGTCTAGGAACGGTACGATCGTATTCCAAGGACTTTGCTGGTGGTATCC
Target unique ID: Other-41
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_971 | H3.15 | Other | HIV reverse transcriptase (HIV RT) | 2.05 nM | 20 mM Tris-HCl [pH 7.4], 140 mM NaCl, 5 mM KCl, 1 mM MgCl2, and 1 mM CaCl2 | 32298104 |
Structure information of aptamer
| Aptamer Sequence: | TAGTCTAGGAACGGTACGATCGTATTCCAAGGACTTTGCTGGTGGTATCC |
| The optimal secondary structure in dot-bracket notation: | .(((((.((((((((...)))))..)))..)))))............... |
| The centroid secondary structure in dot-bracket notation: | .(((((.((((((((...)))))..)))..)))))............... |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_971 | Description | H3.15 |
| Aptamer chemistry | DNA | Length | 50 nt |
| GC content | 48.0% | Molecular weight | 15,446.91 Da |
| Molarity of 1 μg/μl solution | 64.74 μM | Number of G-quadruplexes | No QGRS found |
| G-Score | N/A | Function | Target recognition |
| Sequence | TAGTCTAGGAACGGTACGATCGTATTCCAAGGACTTTGCTGGTGGTATCC | ||
| Applications | Binding to target proteins | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Target ID | Other-41 | Name | HIV reverse transcriptase (HIV RT) |
Activity data
 |
Interaction ID | 1054 |
| Target type | Other | |
| Target unique ID | Other-41 | |
| Activity | 2.05 nM | |
| Binding Conditions/Buffer | 20 mM Tris-HCl [pH 7.4], 140 mM NaCl, 5 mM KCl, 1 mM MgCl2, and 1 mM CaCl2 |
|
| Assay | MST binding affinity curves for the affinity aptamers |
|
| PubMed ID | 32298104 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_972 | DNA | TGACAGTACGTACTGAACTAATTTATAAGTAATTTGCTATGGTGGTATCC | |
| Apta_973 | DNA | TATGTAATGTTGACGTAATTTATCTCTGTGAGAATAAGCAGGTGGTATCC | |
| Apta_975 | DNA | AGACATAGTACATAACCGATCAATGAACATTTAATTAAATGGTGGTATCC | |
| Apta_977 | DNA | CAATCTGCTAAAACTTAAAGCCTATTAATGAAATTGTTAAGGTGGTATCC | |
| Apta_267 | DNA | GAGTGCGTAATGGTACGATTTGGGAAGTGGCTTGGGGTGG | |
| Apta_970 | DNA | TTATGTAGCATTTATGAAATTTTTAAATCAATTTACTATTGGTGGTATCC | |
| Apta_697 | DNA | CTCTCGGGACGACGGACGCTAATCTTACAAGGGCGTAGTGTATGTCGTCCC | |
| Apta_220 | DNA | ATCAACTCACCGTAGGTCTAGTGAGAACTTCAAGTCTACT | |
| Apta_275 | DNA | AGTTGAATGGTGTGGTCACTTCCAGTCCCGCAGGGCACAC | |
| Apta_309 | DNA | TAGCCAAGGTAACCAGTACAAGGTGCTAAACGTAATGGCTTCGGCTTAC | |
| Apta_556 | DNA | GGCTCTCGGGACGACATGGATTTTCCATCAACGAAGTGCGTCCGTCCC | |
| Apta_776 | DNA | AGTGGTCGAACTACACATCCTTGAACTGCGGAATTATCTAC | |
| Apta_974 | DNA | CATGGTGTCAATACTATACCTTTTGCCGATGGTAACAGTTGGTGGTATCC | |
| Apta_309 | DNA | TAGCCAAGGTAACCAGTACAAGGTGCTAAACGTAATGGCTTCGGCTTAC | |
| Apta_721 | DNA | CTCTCGGGACGACCGCGGTAGTCTTAACCTAAAGCGGTGTCAGGTCGTCCC | |
| Apta_1109 | DNA | GGTATGTGTCGTTGATCTGGATATCTATGCCTAATGCTCGTAGGCGACTGTTCGGATAGTAACGTTGACCGCATAC | |
| Apta_47 | DNA | GACCTAGCAGTGGACATGTGGCAGGGTGAAGTGGCATCGTC | |
| Apta_263 | DNA | AGAGGGGAGGGTCGGGTATCGGCGTGTTCGGGGGATCTGC | |
| Apta_274 | DNA | GATACTGAGCATCGTACATGATCCCGCAACGGGCAGTATT | |
| Apta_308 | DNA | TGGCGGTACGGGCCGTGCACCCACTTACCTGGGAAGTGA |
DEV TOOL
 |
 |
 |
 |