Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 388
Aptamer sequence: GGCGUGACCUGAUGAGUCACGCAGACGCUAGCGAAUUGGUUCCUCGAAAGGGGAAAGCGUUAUUAAGAAACCAAAAUGUGUUACGAAACGUUCCC
Target unique ID: P28482
Aptamer ID Aptamer descriptor Target chemistry Target name Affinity Binding Conditions/Buffer PubMed ID
Apta_382 N Protein MK01_HUMAN N/A 10 mM Tris-HCl, pH 7.5, 10 mM MgCl2, 0.05 μg/μl tRNA 12118241
Structure information of aptamer
Aptamer Sequence: GGCGUGACCUGAUGAGUCACGCAGACGCUAGCGAAUUGGUUCCUCGAAAGGGGAAAGCGUUAUUAAGAAACCAAAAUGUGUUACGAAACGUUCCC
The optimal secondary structure in dot-bracket notation: .(((((((.......))))))).((((((..........((((((....)))))))))))).............(((((........)))))...
The centroid secondary structure in dot-bracket notation: .(((((((.......))))))).((((((..........((((((....)))))))))))).............(((((........)))))...
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
Type Detail Type Detail
Aptamer ID Apta_382 Description N
Aptamer chemistry RNA Length 95 nt
GC content 48.4% Molecular weight 30,687.30 Da
Molarity of 1 μg/μl solution 32.59 μM Number of G-quadruplexes No QGRS found
G-Score N/A Function Targeted binding
Sequence GGCGUGACCUGAUGAGUCACGCAGACGCUAGCGAAUUGGUUCCUCGAAAGGGGAAAGCGUUAUUAAGAAACCAAAAUGUGUUACGAAACGUUCCC
Applications Monitoring post-translational modification of proteins
Target information
Type Detail Type Detail
Uniprot ID P28482 Protein name Mitogen-activated protein kinase 1 (MAP kinase 1) (MAPK 1) (EC 2.7.11.24) (ERT1) (Extracellular signal-regulated kinase 2) (ERK-2) (MAP kinase isoform p42) (p42-MAPK) (Mitogen-activated protein kinase 2) (MAP kinase 2) (MAPK 2)
Gene name(s) MAPK1 ERK2 PRKM1 PRKM2 Organism Mitogen-activated protein kinase 1 (MAP kinase 1) (MAPK 1) (EC 2.7.11.24) (ERT1) (Extracellular signal-regulated kinase 2) (ERK-2) (MAP kinase isoform p42) (p42-MAPK) (Mitogen-activated protein kinase 2) (MAP kinase 2) (MAPK 2)
Status reviewed in uniprot database Length 360
Mass 41,390 Activity regulation Phosphorylated by MAP2K1/MEK1 and MAP2K2/MEK2 on Thr-185 and Tyr-187 in response to external stimuli like insulin or NGF
Involvement in disease Noonan syndrome 13 (NS13) Catalytic activity Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein]
PDB ID(s)
1PME;1TVO;1WZY;2OJG;2OJI;2OJJ;2Y9Q;3D42;3D44;3I5Z;3I60;3SA0;3TEI;3W55;4FMQ;4FUX;4FUY;4FV0;4FV1;4FV2;4FV3;4FV4;4FV5;4FV6;4FV7;4FV8;4FV9;4G6N;4G6O;4H3P;4H3Q;4IZ5;4IZ7;4IZA;4N0S;4NIF;4O6E;4QP1;4QP2;4QP3;4QP4;4QP6;4QP7;4QP8;4QP9;4QPA;4QTA;4QTE;4XJ0;4ZXT;4ZZM;4ZZN;4ZZO;5AX3;5BUE;5BUI;5BUJ;5BVD;5BVE;5BVF;5K4I;5LCJ;5LCK;5NGU;5NHF;5NHH;5NHJ;5NHL;5NHO;5NHP;5NHV;5V60;5V61;5V62;5WP1;6D5Y;6DMG;6G54;6G8X;6G91;6G92;6G93;6G97;6G9A;6G9D;6G9H;6G9J;6G9K;6G9M;6G9N;6GDM;6GDQ;6GE0;6GJB;6GJD;6NBS;6OPG;6OPH;6OPI;6Q7K;6Q7S;6Q7T;6QA1;6QA3;6QA4;6QAG;6QAH;6QAL;6QAQ;6QAW;6RQ4;6SLG;7AUV;7E73;7E75;7NQQ;7NQW;7NR3;7NR5;7NR8;7NR9;7OPM;7W5O;
Function Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway.
Activity data

No relevant experimental diagram

 Interaction ID 388
Target type Protein
Target unique ID P28482
Activity N/A
Binding Conditions/Buffer

10 mM Tris-HCl, pH 7.5, 10 mM MgCl2, 0.05 μg/μl tRNA
Assay

N/A
PubMed ID 12118241
Similar aptamers
Aptamer ID Aptamer chemistry Sequence Similarity
Apta_383 RNA GGCGUGACCUGAUGAGUCACGCUAAGGAGGAUUUCCGAAAGCGGCUACGGUCCGCCAGUGUUACGAAACGUUCCC 69.47%
Apta_6 RNA GGAAAGACGCUAGCGAAUUGGUUCCUCGAAAGGGGAAAGCGUUAUUAAGAAACCAAAAUUUCC 66.32%
Apta_5 RNA GGGAGAGCCAUACCUGACAAAGACGCUAGCGAAUUGGUUCCUCACUCAAAAGUAGGGGAAAGCGUUAUUAAGAAACCAAAAUUUGACAGGUUACGCAUCCUGCAUCCUCCAGGUUACGCAUCC 64.23%
Apta_1222 RNA GGAGGCGCCAACUGAAUGAAACCGUGCUAAGGAGUUGGACUCCGCUGCUUCGGCAGAGCGAUAUAGUGCCAGCUGAUACAUCCGUAACUAGUCGCGUCAC 53.00%
Apta_1226 RNA GGAGGCGCCAACUGAAUGAAGGUGUAUUUCAGUUAAAGAACUUGCUGCUUCGGCAGCGUUCUGAGUUACUAGUCCAAUAAUCCGUAACUAGUCGCGUCAC 53.00%
Apta_453 RNA GGGCUAGCUGAUCGUACCAGUAGCGUGGCCUGGGGGGCCUAGUCGUGCGAUACUAACAGCUAACACCC 52.63%
Apta_1057 RNA UCUGAGGGGUUGGGGCCGCAGUCUCGUCGCCAUGGCUAACGAGGCCGGAACUAGUUUUAGCCGAAAUGAUCGAUGC 52.63%
Apta_280 RNA GAUAAUACGACUCACUAUAGGGUUCACUGCAGACUUGACGAAGCUUGCAGAAAAGGGGGAAGAAGAGGGUGAUUCAGGCGAGAGAAUGGAUCCACAUCUACGAAUUC 52.34%
Apta_170 RNA GGGAGACAAGACUAGACGCUCAAUGUGGGCCACGCCCGAUUUUACGCUUUUACCCGCACGCGAUUGGUUUGUUUCCC 51.58%
Apta_522 RNA GGGCGACCCUGAUGAGCACACGAGGGGGAAACCCCGGACAAUCAGACACGGUGUUCGAAACGGUGAAAGCCGUAGGUUGCCCUUU 51.58%
Apta_575 RNA AGAGCUUGACGGUCCCGAGAGUCGAGCCCAAGCUGACACUGGACCUUUGCGGACCACGUGUUGAUCGUCG 51.58%
Apta_598 RNA GGGAAUUCCGCGUGUGCGCCGCGGAAGACGUUGGAAGGAUAGAUACCUACAACGGGGAAUAUAGAGGCCAGCACAUAGUGAGGCCCUCCUCCC 51.58%
Apta_612 RNA GGGCGACCCUGAUGAGCCUUAGGAUAUGCUUCGGCAGAAGGACGUCGAAACGGUGAAAGCCGUAGGUUGCCC 51.58%
Apta_723 RNA GGGAGCUCAGAAUAAACGCUCAAAUCCGUGGACAGGGCGUAAGCGCCUUCGACAUGAGACACGGAUCCUGCGACGAAUUCAGC 51.58%
Apta_549 RNA GGAGCUCAGCCUUCACUGCAUGAUAAACCGAUGCUGGGCGAUUCUCCUGAAGUAGGGGAAGAGUUGUCAUGUAUGGGGGCACCACGGUCGGAUCCUG 51.55%
Apta_1224 RNA GGAGGCGCCAACUGAAUGAAUGUUCUGGAUCUGCUAGAGACUAUCUGCUUCGGCAGCACUCUCCGUUAACAACACGUACAUCCGUAACUAGUCGCGUCAC 51.00%
Apta_1225 RNA GGAGGCGCCAACUGAAUGAAAGAGGUAACGAAAGAUGAUUGUGCCUGCUUCGGCAGUACAACUUCUCGGAUAAAUGAUUAUCCGUAACUAGUCGCGUCAC 51.00%
Apta_494 RNA GGGAGAUACCAGCUUAUUCAAUUCUGGCAAUGGGCUAUCCCAAGUGCUAGGCUUCAGGGAGCGAGGACCAGACGACGUACCUAACCCUAAGGUGAGAUAGUAAGUGCAAUCU 50.89%
Apta_80 RNA GCAAUGGUACGGUACUUCCUUUGGAAGAUAGCUGGAGAACUAACCAAAAGUGCACGCUACUUUGCUAA 50.53%
Apta_224 RNA GGGGCCACCAACGACAUUGAACCACGUAGGCUCGUUUCUGAGCCGAUCUCGAUGUUGAUAUAAAUAGUGCCCAUGGAUC 50.53%

ABOUT

AptaDB is an online database we developed to integrate comprehensive information about aptamer.
The detail of this database is described here:

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