Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 224
Aptamer sequence: ATCAACTCACCGTAGGTCTAGTGAGAACTTCAAGTCTACT
Target unique ID: P05121
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_220 | R9chWT-15 | Protein | PAI1_HUMAN | 177 pM | 20 mM HEPES (pH 7.4), 50 mM NaCl, 2 mM CaCl2, and 0.1% BSA | 19284310 |
Structure information of aptamer
| Aptamer Sequence: | ATCAACTCACCGTAGGTCTAGTGAGAACTTCAAGTCTACT |
| The optimal secondary structure in dot-bracket notation: | ...........(((((.(((((....)))...))))))). |
| The centroid secondary structure in dot-bracket notation: | ...........(((((.((.............))))))). |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_220 | Description | R9chWT-15 |
| Aptamer chemistry | DNA | Length | 40 nt |
| GC content | 42.5% | Molecular weight | 12,478.84 Da |
| Molarity of 1 μg/μl solution | 80.14 μM | Number of G-quadruplexes | No QGRS found |
| G-Score | N/A | Function | Targeted therapy |
| Sequence | ATCAACTCACCGTAGGTCTAGTGAGAACTTCAAGTCTACT | ||
| Applications | Adjuvant to traditional chemotherapy for breast cancer | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Uniprot ID | P05121 | Protein name | Plasminogen activator inhibitor 1 (PAI) (PAI-1) (Endothelial plasminogen activator inhibitor) (Serpin E1) |
| Gene name(s) | SERPINE1 PAI1 PLANH1 | Organism | Plasminogen activator inhibitor 1 (PAI) (PAI-1) (Endothelial plasminogen activator inhibitor) (Serpin E1) |
| Status | reviewed in uniprot database | Length | 402 |
| Mass | 45,060 | Activity regulation | N/A |
| Involvement in disease | Plasminogen activator inhibitor-1 deficiency (PAI-1D) | Catalytic activity | N/A |
| PDB ID(s) | 1A7C;1B3K;1C5G;1DB2;1DVM;1DVN;1LJ5;1OC0;3CVM;3EOX;3PB1;3Q02;3Q03;3R4L;3UT3;4AQH;4G8O;4G8R;4IC0;5BRR;5ZLZ;6GWN;6GWP;6GWQ;6I8S;6ZRV;7AQF;7AQG;9PAI; |
 |
Function | Serine protease inhibitor. Inhibits TMPRSS7. |
Activity data
|
No relevant experimental diagram |
Interaction ID | 224 |
| Target type | Protein | |
| Target unique ID | P05121 | |
| Activity | 177 pM | |
| Binding Conditions/Buffer | 20 mM HEPES (pH 7.4), 50 mM NaCl, 2 mM CaCl2, and 0.1% BSA |
|
| Assay | N/A |
|
| PubMed ID | 19284310 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_190 | DNA | AGGAACCCTTTAGGTCTGAGATCTCGGAAT | |
| Apta_1162 | DNA | AGAGGCTACAGCGATAAGTCGACATTGCTGACCGTACCTAGTAATACGT | |
| Apta_65 | DNA | CTACCGTGGTAGGGAAGGTTGGAGTGTAG | |
| Apta_72 | DNA | AGTCAGTAGGTTGGTGTGGTTGGGGCTGACT | |
| Apta_185 | DNA | ACCAAGGCTTTAGGTCTGAGATCTCCCAAT | |
| Apta_186 | DNA | AGGAACGCTTTAGGTCTGAGATCTCGGAAT | |
| Apta_187 | DNA | ACCAACCCTTTACCTCTGAGATCTCCCAAT | |
| Apta_335 | DNA | ACCGGGCGTACACCGTCGCGGCACATGTCTGAATGCGTTTAGTCTCTGTG | |
| Apta_971 | DNA | TAGTCTAGGAACGGTACGATCGTATTCCAAGGACTTTGCTGGTGGTATCC | |
| Apta_977 | DNA | CAATCTGCTAAAACTTAAAGCCTATTAATGAAATTGTTAAGGTGGTATCC | |
| Apta_969 | DNA | TAACCACCCCACCTCGCTCCCGTGACACTAATGCTAATTCCAA | |
| Apta_820 | DNA | TCCCACGATCTCATTAGTCTGTGGATAAGCGTGGGACGTCTATGA | |
| Apta_833 | DNA | ATTTCGCCCCCGTGTTCCGACTGGTATCTTCACGTCTTCGAGTGT | |
| Apta_309 | DNA | TAGCCAAGGTAACCAGTACAAGGTGCTAAACGTAATGGCTTCGGCTTAC | |
| Apta_309 | DNA | TAGCCAAGGTAACCAGTACAAGGTGCTAAACGTAATGGCTTCGGCTTAC | |
| Apta_66 | DNA | TACCGTGGTAGGGAAGGTTGGAGTGTA | |
| Apta_166 | DNA | ATCGCGCTCTCCTGCTTAAGCAGCTATCAAATAGCCCACT | |
| Apta_184 | DNA | AGGAAGGCTTTAGGTCTGAGATCTCGGAAT | |
| Apta_188 | DNA | AAAAAAACTTTAAATCTGAGATCTCAAAAT | |
| Apta_189 | DNA | AGGAACCCTTTACCTCTGAGATCTCGGAAT |
DEV TOOL
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